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Method for manufacturing slices of plant leaves

A production method and plant leaf technology, applied in the biological field, can solve the problems of interfering with the observation of pathogenic microorganisms, affecting the quality of experimental pictures, shortening the observation time, etc., and achieving the effects of low toxicity, convenient operation, and reduction of dehydration and shrinkage.

Active Publication Date: 2017-02-22
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, frozen sections are easy to dry quickly after thawing into slices, which will deform the tissue and shorten the observation time rapidly. The quality of the observed experimental pictures

Method used

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  • Method for manufacturing slices of plant leaves
  • Method for manufacturing slices of plant leaves
  • Method for manufacturing slices of plant leaves

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Embodiment 1

[0014] Citrus bacterial canker disease ， CBCD) is a major disease affecting the development of the global citrus industry, and its pathogen is Xanthomonas citri var. Xanthomonas citri susp. citri, Xcc). After successfully attaching to the host surface, canker bacteria must invade plant tissue in order to be able to cause disease. Therefore, the process of pathogenic bacteria invading hosts is the key link in pathogenicity. Observing the process of canker infection and colonization in citrus hosts is of great significance for the prevention and control of canker and the selection of disease-resistant varieties. In order to observe the infection and colonization process of canker pathogens in citrus hosts, it is necessary to make slices of plant leaves and observe the position of canker disease in leaves from longitudinal and transverse sections.

[0015] In this example, the canker bacteria solution was inoculated on the surface of the rock sugar orange leaves, and the in...

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PUM

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Abstract

The invention discloses a method for manufacturing slices of plant leaves. Aqueous solution with a nonionic surfactant tween-20 is added in slice manufacturing procedures. The volume concentration of the tween-20 in the aqueous solution with the nonionic surfactant tween-20 is lower than 0.1%, and the usage of the aqueous solution used for manufacturing the slices ranges from 50 micro-l to 100 micro-l. The method has the advantages that the aqueous solution with the nonionic surfactants--tween-20 is added, so that the activity of materials can be kept, and dehydration and shrinkage of the materials can be reduced to the greatest extent; the effective observation time of the compressed slices made from fluorescent bacteria with markers can reach 18 h when the materials are about to be observed by laser confocal microscopes or other microscopes, fluorescence can be kept for 2 days, and observed images are almost free of auto-fluorescence of the plant leaves; only the aqueous solution with the tween-20 needs to be added when the slices are manufactured by the aid of the method for manufacturing the slices of the plant leaves, and accordingly the method is particularly suitable for manufacturing slices of citrus leaves and is easy to implement.

Description

technical field [0001] The invention relates to a method for making plant leaf slices, in particular to a method for prolonging the microscopic observation time of slices and reducing the interference of material autofluorescence, which belongs to the field of biotechnology. Background technique [0002] When microscopically observing plant tissue cells, it is necessary to slice or compress the observation materials. It is required that no damage should be caused to the observation materials during the production of plant materials, especially when observing pathogenic microorganisms invading the host. Normal viability should not confuse the plant tissue damage caused by the production with the damage caused by the pathogen infection. Therefore, the production method that ensures that the host and microorganisms are not damaged is very important for observing the infection process of the pathogen. [0003] At present, the commonly used sectioning method is paraffin sectionin...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 邓子牛赵亚马先锋戴素明符红艳李芳
Owner HUNAN AGRICULTURAL UNIV
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