77 results about "Continuous perfusion" patented technology

A catheter for delivering a therapeutic or diagnostic agent to a branch blood vessel of a major blood vessel, generally comprising an elongated shaft having at least one lumen in fluid communication with an agent delivery port in a distal section of the shaft, an expandable tubular member on the distal section of the shaft, and a radially expandable member on the tubular member. The tubular member is configured to extend within the blood vessel up-stream and down-stream of a branch vessel, and has an interior passageway which is radially expandable within the blood vessel to separate blood flow through the blood vessel into an outer blood flow stream exterior to the tubular member and an inner blood flow stream within the interior passageway of the tubular member. The radially expandable member is located down-stream of the shaft agent delivery port, and has an expanded configuration with an outer diameter larger than an outer diameter of the tubular member. The expanded radially expandable member is configured to decrease the blood flow in the outer blood flow stream down-stream of the branch vessel. The catheter provides for delivery of an agent to a branch vessel of a major vessel, and continuous perfusion of the major blood vessel. Another aspect of the invention is directed to methods of delivering a therapeutic or diagnostic agent to one or both kidney's of a patient.

A removable, low profile, vascular filter system for capture and retrieval of emboli while allowing continuous perfusion of blood, comprising a guidewire having proximal and distal markers, a filter delivery and deployment system, having an outer member and an inner member, and a filter removably attached near the distal end of the inner member. The filter comprises proximal and distal basket sleeves which position the filter between the proximal and distal markers on the guidewire, after the guidewire is in position past the vessel occlusion. The guidewire placement prior to filter delivery may facilitate access to the interventional site, especially through tortuous anatomy. Also, retrievability of the filter from the wire may avoid loss of guidewire position, which may occur following removal of filters permanently attached to guidewires.

A removable vascular filter system for capture and retrieval of emboli while allowing continuous perfusion of blood, comprising a porous filter membrane and a filter membrane support structure. This system is useful for any percutaneous angioplasty, stenting, thrombolysis or tissue ablation procedure. The system may minimize the incidence of stroke, myocardial infarction or other clinical complications that may be associated with these procedures.

The present invention relates to a process and apparatus for purifying a molecule of interest from a heterogeneous clarified fluid mixture. The apparatus of the invention generally comprises a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system; and a continuous purification system integrated with the particle removal system, which is maintained under sterile conditions. The process comprises filtering a heterogeneous clarified fluid mixture by continuous ultrafiltration at a specific flow rate below the transition point of the molecule of interest in the pressure-dependent region of the flux versus TMP curve, wherein the specific flow rate is maintained substantially constant throughout the continuous ultrafiltration.

A removable vascular filter system for capture and retrieval of emboli while allowing continuous perfusion of blood. This system is useful for any percutaneous angioplasty, stenting, thrombolysis or tissue ablation procedure. The system may minimize the incidence of stroke, myocardial infarction or other clinical complications that may be associated with these procedures.

A removable vascular filter system for capture and retrieval of emboli while allowing continuous perfusion of blood during a cardiopulmonary bypass procedure, comprising a porous filter membrane with variable diameter holes, and a filter membrane support structure. The system may minimize the incidence of stroke, myocardial infarction or other clinical complications that may be associated with cardiopulmonary bypass procedures.

A balloon catheter for dilation of stenotic tissue within a tubular member of the body such as a blood vessel provides fluid flow such as blood flow through the balloon while it is being inflated and after it has been inflated. For valvuloplasty applications or for predilitation prior to a TAVI procedure the balloon is placed across the stenotic aortic valve leaflets and inflated to push the leaflets aside to create a greater blood flow area or allow improved subsequent passage of the large TAVI catheter across the stenotic aortic valve leaflets. As the balloon is inflated in diameter, it is required to reduce in length; this length reduction causes a braided inner tubing to also reduce in length. The braided inner tube is thereby required it to increase in diameter during the balloon inflation and create a suitably large blood flow path through the inner tubing. The continuous perfusion through the inner tubing of the balloon allows greater balloon inflation times, improved valvular dilation, and obviates the need for rapid pacing during the valvuloplasty procedure.

A perfusion chamber includes a porous oocyte support structure. A continuously sloped top surface and a receiving well in the support structure entrap the underside of the oocyte, thereby localizing the cell in a predetermined fixed position within the reach of dedicated voltage-clamp microelectrodes. A test solution is delivered continuously at the top of the chamber, above the oocyte, and withdrawn from the bottom of the chamber, below the oocyte. The porosity of the support material enables the continuous perfusion of test solution around the membrane of the oocyte, including its bottom portion that is held firmly in contact with the holding well. The geometry of the holding well is selected such as to ensure the automatic and precise placement of the oocyte by gravity and to optimize the pressure distribution over its membrane, thereby minimizing the probability of rupture or other damage to the cell. The entire top surface of the support structure is sloped inward toward the substantially central well, and the bottom of the well is contoured to conform to the shape of an oocyte of average size.

A removable vascular filter system for capture and retrieval of emboli while allowing continuous perfusion of blood during a carotid endarterectomy procedure, comprising a porous filter membrane with variable diameter holes, and a filter membrane support structure. The system may minimize the incidence of stroke, or other clinical complications that may be associated with carotid endarterectomy procedures.

The invention discloses a method to mass produce flu vaccine with bioreactor, which comprises the following steps: proceeding passage adaptation on Africa green monkey kidney cell (Vero cell) with influenza virus; intensifying intrinsic appeal of influenza virus for Vero cell; fitting for Vero cell quickly; breeding the Vero cell with cell density above 10. 7deka / ml in bioreactor; seeding the influenza virus into bioreactor; adjusting various controlling parameter in the reactor; making the virus high effective breed in the reactor; proceeding continuous perfusion; harvesting virus liquid; deactivating the virus; condensing; purifying; cracking; getting the flu vaccine. This invention possesses the advantages of high antigen quantity and stable quality.

Efforts to extend myocardial preservation for transplantation by perfusion with prior crystalloid based solutions have been limited by edema and compromised function. Hypothermic perfusion preservation with a polyethylene glycol (PEG) conjugated hemoglobin solution extends preservation times. The polyethylene glycol (PEG) conjugated hemoglobin solution comprises PEG-Hb, and at least one of the constituents selected from the group of human albumin, dextrose, heparin sodium, lidocaine HCl, MgSO4, KCl, CaCl2, Tromethamine (THAM) solution, NaCl, NaHCO3, and Na2HPO4 / NaH2PO4. Comparison of cardiac function after continuous perfusion using a hypocalcemic normokalemic crystalloid perfusate is made with and without the addition of PEG-Hemoglobin (Hb).

A placenta original filled stem cell and its production are disclosed. The process is carried out by bleeding after delivering placenta from uterus, perfusing to obtain single-cell soliquoid from continuous perfusion method or collagenase digestion method and acquiring filled stem cell from gradient and density centrifugation or immune magnetic bead separation. It has better external enrichment potentiality and multidirectional differential potentiality and low immunogenicity.

A microplate for culturing cells with automatic, continuous perfusion includes a well frame and a planar substrate. The well frame and the planar substrate form a first well, a second well, and a third well. The first well is fluidly connected with the second well with a first perfusion membrane and the first well is fluidly connected with the third well with a second perfusion membrane. Methods of fabricating the microplate and methods of culturing cells are also provided.

The invention relates to a construction method of a tissue engineering blood vessel which belongs to the tissue engineering field. The construction method of the tissue engineering blood vessel includes the following steps: (1) tubular autologous living tissue bed frame material is connected to a continuous perfusion bioreactor under the vitro aseptic condition, and the outside of a vessel lumen is filled with culture medium; (2) endothelial cells obtained from the vitro culture medium is suspended at M199 or vascular endothelium-like cells generating from the induction of bone marrow mono nucleus cells are suspended at endothelial progenitor cell culture medium and are inoculated on the inner surface of the vessel lumen according to a high density planting method; (3) after the endothelial cells or the vascular endothelium-like cells are adhered, the inner cycle and the external cycle of the vessel lumen are communicated and the blood circulation is simulated to provide proper pressure stimulation and shear stress stimulation and the endothelial cells or the vascular endothelium-like cells are cultured for 5-7 days in a carbon dioxide incubator. The construction method has the advantages that perfect cell compatibility and enough mechanical strength are provided, the defects of immunological rejection and inflammation reaction, etc. caused by heterogenetic material are overcome, and the preparation time is short.

The invention provides a method for preparing a rotavirus vaccine stock solution by using serum-free Vero cells. The method comprises the following steps: culturing Vero cells by using a serum-free culture medium, thus obtaining serum-free culture medium adapted cell strains; establishing a serum-free Vero cell seed bank by utilizing the obtained serum-free culture medium adapted cell strains; establishing a serum-free rotavirus strain working seed bank by utilizing the obtained serum-free culture medium adapted cell strains; carrying out reviving, culturing, passage and amplification on cells in a Vero cell working seed bank by utilizing the serum-free culture medium, using the cells in the Vero cell working seed bank as basic cells cultured in a bioreactor, and carrying out continuous perfusion culture on high-density Vero cells by applying the bioreactor and a microcarrier and using the serum-free culture medium after cell amplification; after inoculating virus seeds in the rotavirus strain working seed bank, carrying out bioreactor-microcarrier serum-free culture, obtaining a virus solution when virus is amplified to the summit, obtaining liquid virus titer, and carrying out clarification and ultra-filtration concentration, thus obtaining a serum-free rotavirus stock solution for human.

The invention relates to a device for preparing stem cells through a continuous perfusion bioreactor / culture bottle (bag) system. The device comprises a culture solution storage tank / bag, a monitored bioreactor, a central cell culture warehouse, a waste liquor / obtained liquor collecting container, physicochemical and biochemical indicator detecting head groups, an online monitoring system and a sterile filter membrane filter, which are connected by a speed-controlled pipeline system of a fluid-controlled sterile driver. The connecting relationships are that: the culture solution storage tank / bag is connected with the monitored bioreactor through the speed-controlled pipeline system; the monitored bioreactor is connected with an inlet of the central cell culture warehouse through the speed-controlled pipeline system; an outlet of the central cell culture warehouse is connected with the waste liquor / obtained liquor collecting container through the speed-controlled pipeline system; the monitored bioreactor is connected to the online monitoring system through a physicochemical and biochemical indicator detecting head group of the reactor; and the online monitoring system is connected with the physicochemical and biochemical indicator detecting head groups at the inlet and outlet of the central cell culture warehouse respectively.

Methods and compositions are provided for the introduction and washout of vitrifiable concentrations of cryoprotectant in organs and tissues. The methods comprise cooling the organ to below −10° C. by perfusion with a solution having a freezing point below −10° C., a temperature from −10 to −40° C., and a tonicity from 1.1 to 2.0 times isotonic, after previous perfusion with said solution for a time insufficient for approximate osmotic equilibration of the organ with the solution. The methods further comprise increasing the concentration of cryoprotectant further at a temperature from −10 to −40° C. to prepare the organ or tissue for vitrification. The methods further comprise cooling and vitrifying the organ, rewarming it, and perfusing the organ with a vitrifiable concentration of cryoprotectant that is the same as or less than the concentration used for vitrification, without the addition of an osmotic buffering agent. Rewarming is accomplished either by rapid (>1°C. / min, and preferably −0.2-20° C. / min) elevation of arterial perfusate temperature from below −20° C. to above −15° C. during continuous perfusion of the organ or by perfusing the organ with pre-warmed arterial perfusate at >−15° C. Extraordinarily effective multicomponent compositions are also provided for the process, particularly involving a vitrification solution whose warming rate after vitrification can be <1° C. / min without freezing during rewarming and a chilling injury protective solution having zero toxicity to whole organs at 0° C. and permitting almost complete avoidance of chilling injury at −20 to −25° C.

The present invention relates to a process and apparatus for purifying a molecule of interest from a heterogeneous clarified fluid mixture. The apparatus of the invention generally comprises a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system; and a continuous purification system integrated with the particle removal system, which is maintained under sterile conditions. The process comprises filtering a heterogeneous clarified fluid mixture by continuous ultrafiltration at a specific flow rate below the transition point of the molecule of interest in the pressure-dependent region of the flux versus TMP curve, wherein the specific flow rate is maintained substantially constant throughout the continuous ultrafiltration.

A cell depositing interception device of hematopoietic cell continuous perfusion cultivation bioreactor system, comprises an inclined tube-form depressor with tube-form cross-section, the upper portion of which is arranged with a feed inlet, a discharge port is arranged on the bottom of the depressor corresponding to the feed inlet position, the upper surface of the other end is provided with at discharge port and an exhaust port.

The invention discloses a single-chamber reciprocating blood pump with magnetic and elastic linkage. The technical scheme includes that the single-chamber reciprocating blood pump with magnetic and elastic linkage comprises a pump body, a storage cavity for blood storage is arranged in the pump body, the storage cavity gets larger or smaller with the change of compression, the pump body is externally coated and wound with a conductive coil, a magnet sliding along the lengthwise direction of the pump body is arranged in the inner cavity of the pump body, one end of the magnet is connected withthe storage cavity in an abutted manner, the magnet slides relative to the pump body to squeeze the storage cavity, one end of the magnet, away from the storage cavity is provided with a tension spring, and the conductive coil drives the magnet to move in a reciprocating manner along the lengthwise direction of the pump body in power supply or power failure. By the arrangement, the rhythm of heartbeat is simulated, and injury to target organs by continuous perfusion can be avoided.

The invention relates to a preparation method and a product of a porcine circovirus type 2 inactivated vaccine, and belongs to the technical field of biological products. The preparation method of the porcine circovirus II-type inactivated vaccine disclosed by the invention comprises the steps of 1) implementing multiplication culture on a PK-15 cell; 2) inoculating virus to culture and prepare a virus solution; and 3) condensing and inactivating the virus, and preparing a finished vaccine product. The preparation method disclosed by the invention, which amplifies PCV-2 (porcine circovirus-2) through a continuous perfusion-type bioreactor, has the advantages of being relatively small in operation space, simple in operation process, remarkably low in labor, capable of being used for mass production through cascade amplification, and being low in serum content in maintenance media, relatively low in production cost and high in product quality controllability; the prepared porcine circovirus type 2 inactivated vaccine has the advantages of high safety, good immune effect, low side effect and the like.

A balloon catheter for dilation of stenotic tissue within a tubular member of the body such as a blood vessel provides fluid flow such as blood flow through the balloon while it is being inflated and after it has been inflated. For valvuloplasty applications or for predilitation prior to a TAVI procedure the balloon is placed across the stenotic aortic valve leaflets and inflated to push the leaflets aside to create a greater blood flow area or allow improved subsequent passage of the large TAVI catheter across the stenotic aortic valve leaflets. As the balloon is inflated in diameter, it is required to reduce in length; this length reduction causes a braided inner tubing to also reduce in length. The braided inner tube is thereby required it to increase in diameter during the balloon inflation and create a suitably large blood flow path through the inner tubing. The continuous perfusion through the inner tubing of the balloon allows greater balloon inflation times, improved valvular dilation, and obviates the need for rapid pacing during the valvuloplasty procedure.

The invention provides a perfusion culture chip and a perfusion system. The perfusion culture system comprises an upper sealing plate, a culture filter layer and a lower sealing plate layer, the lowersealing plate is provided with an injection interface and a filter-out interface, a filling channel corresponding to the injection interface penetrates the culture filter layer and communicates withthe upper side wall of a culture chamber in the culture filter layer, and filtering holes in the bottom of the culture chamber correspond to the filtering interfaces in position. According to the present invention, perfusion culture is carried out in a manner of injecting the culture solution from the upper part and filtering out the culture solution from the bottom, so that continuous perfusion culture of microorganisms and cells can be realized.

The invention discloses a centrifugal settling-type bioreactor for continuous culture of plant cells. The centrifugal settling-type bioreactor comprises a main body which is in a U-shaped structure on the whole; a liquid inlet is also formed in one side of a liquid lifting region; a cell harvest opening is formed in the bottom position of a liquid dropping part; a liquid lifting part is provided with a gas nozzle at the bottom position of the main body; a seal funnel and a spiral pipe are arranged on the liquid dropping part from top to bottom; the top part of the seal funnel and the inner wall of the liquid dropping part of the main body are in fit seal; the bottom part of the seal funnel is connected to the inlet of the spiral pipe; a gravity settling pipe is also connected to the bottom part of the spiral pipe; and the other side of the gravity settling pipe is connected with a waste liquid collector. Separation of cells and a culture solution is achieved by the gravity settling pipe, which is connected with the bottom of the spiral pipe, in the bioreactor; the cell intercept efficiency can be obviously improved by adding the spiral pipe to the reactor, so that continuous perfusion culture of the plant cells is achieved; and the culture efficiency of the plant cells is improved.

The invention discloses a method for large-scale and high density animal cell perfusion culture to produce recombinant protein by a microcarrier technology. The method is characterized in that: a shaking bioreactor is employed, a microcarrier suspends in the reactor and is in an uninterrupted flow state, a continuous perfusion way is adopted for continuous supplement of nutrients and metabolic waste is discharged, and at the same time the recombinant protein is harvested.

Provided is a nerve intervention high-pressure continuous perfusion system and a use method thereof. The perfusion system comprises a fixed rack, an infusion bag conveying device, supports, an infusion bag pressing device and a monitoring and controlling system; the fixed rack is of a strip-shaped annular structure and is horizontally fixed to the ceiling and / or the wall of an operative room; the infusion bag conveying device is used for conveying infusion bags, the infusion bag pressing device is used for pressing the infusion bags and inserting and pulling out liquid inlet needles, the monitoring and controlling system is used for detecting the positions of pulleys in the infusion bag conveying device, the weights of displacement sensors and the infusion bags and the flow of liquid in the pressure bags, and meanwhile controls the work of related components of the infusion bag conveying device and the infusion bag pressing device. The nerve intervention high-pressure continuous perfusion system achieves automatic pressing of the infusion bags, detection of parameters of the infusion bags, automatic replacement of the infusion bags and alarm of abnormal infusion conditions.

The invention discloses a pet drug filling device for veterinary medicine. The structure includes a drug filling headgear, a drug filling head, a front catheter, a front fixing sleeve, a front half pipe of an injection cylinder, a chassis, a rocker, a rotating shaft, a drug storage bottle, a cover plate, Liquid level gauge, rubber sleeve, piston rod, first fixed block, first fixed sleeve, booster rod, second fixed block, second fixed sleeve, limit block, connecting rod, push handle, rear grip rod, positioning pin, Spring, front grip rod, glass liquid storage cylinder, rear half tube of injection cylinder, flow limiting tube, regulator, elastic fixed block, fixed end, the present invention is a veterinary pet drug filling device, which can be conveniently used when in use. Applying force, the process of feeding medicine to pets is easy to operate, and it can be continuously filled until the medicine inside the medicine storage bottle is used up before it needs to be replaced, which can save time to the greatest extent and is easier to operate.

The invention discloses a multifunctional continuous perfusion apparatus, which comprises a residual liquid recovery device, wherein the residual liquid recovery device comprises a main body; a dilution bottle mounting place and a vaccine bottle support mounting place are arranged on the main body; the dilution bottle mounting place and the vaccine bottle support mounting position communicate by virtue of a tube body; a check valve is arranged below the dilution bottle mounting place; an air valve is arranged below the vaccine bottle support mounting place; the check valve and a dilution bottle communicate; and the air valve and a vaccine bottle support communicate. The continuous perfusion apparatus disclosed by the invention, by installing the residual liquid recovery device, can achieve recovery of vaccine residual liquid, so that the loss of vaccine dose is reduced, full mixing of the vaccine and diluted liquid is guaranteed to the greatest extent and the accuracy of dosage is also guaranteed to the greatest extent.

The invention relates to a production method of a fished-product tissue engineered bone. The method includes: acquiring the mononuclear cell of marrow, and performing multiplication culture to obtain single-cell suspension with the concentration of 2.0*10<6>-2.0*10<7> / ml; soaking a porous scaffold into the single-cell suspension to allow the single-cell suspension to enter the porous scaffold sufficiently, and culturing to obtain cell / scaffold composite; inoculating the cell / scaffold composite into a perfusion type bioreactor, adding culture medium and standing for 2-4 hours, and then performing continuous perfusion culture on the cell / scaffold composite to obtain the tissue engineered bone; performing preliminary cleaning, cryopreservation, virus inactivation, ultrasonic cleaning, vacuum freezing drying, aseptic packaging and radiation sterilizing on the tissue engineered bone to obtain the finished-product tissue engineered bone. The method has the advantages that the cryopreservation is performed on the produced tissue engineered bone, and the immunogenicity of the tissue engineered bone can be effectively lowered.

The invention relates to the technical field of cell culture containers for laboratories, and especially relates to a three-dimensional cell culture bottle capable of realizing continuous perfusion. The three-dimensional cell culture bottle comprises a culture bottle body, wherein the inner bottom of the culture bottle body is provided with continuous steps which are gradually lowered; a side wallis arranged on one side of the step surface of each step; a notch is formed in one end of the side wall; protrusions are evenly distributed on the step face, a liquid storage pool is arranged at oneend of the step face at the lowest position of the continuous steps, the step face where the liquid storage pool is located is connected with a supporting plate, the other end of the supporting plateextends to the position of a bottle neck, and an upper filling inlet and a lower flowing outlet are formed in the side wall of the culture bottle body. The three-dimensional cell culture bottle has the beneficial effects that a large-area cell three-dimensional adhesion culture space is created, and the single-bottle cell culture quantity is increased, so that the cell culture cost is indirectly reduced, the sustainable and stable perfusion of a cell culture solution can be realized, the continuous stability of a cell living environment is ensured, and the method is suitable for the growth andamplification of various adherent cells.