Method for producing influenza vaccine in large-scale by using bioreactor

Abstract

The invention discloses a method to mass produce flu vaccine with bioreactor, which comprises the following steps: proceeding passage adaptation on Africa green monkey kidney cell (Vero cell) with influenza virus; intensifying intrinsic appeal of influenza virus for Vero cell; fitting for Vero cell quickly; breeding the Vero cell with cell density above 10. 7deka / ml in bioreactor; seeding the influenza virus into bioreactor; adjusting various controlling parameter in the reactor; making the virus high effective breed in the reactor; proceeding continuous perfusion; harvesting virus liquid; deactivating the virus; condensing; purifying; cracking; getting the flu vaccine. This invention possesses the advantages of high antigen quantity and stable quality.

Description

technical field [0001] The invention relates to a method for large-scale production of influenza vaccine using a bioreactor. Background technique [0002] The influenza vaccine that has been approved for production is still an inactivated vaccine derived from chicken embryos, but it still has many deficiencies, such as, the continuous passage of chicken embryos often causes strain variation; chicken embryos may be contaminated by exogenous virus factors; Chicken embryo production of influenza vaccine can not meet the needs of emergency situations, especially the outbreak of bird flu, will also affect the normal supply of chicken embryos. Therefore, the World Health Organization is urging countries around the world to find ways to mass-produce influenza vaccines from cell lines sensitive to influenza viruses. Chinese Patent No. 02112013.7 discloses a method for large-scale continuous production of virus vaccines in the specification for a patent for invention, which comprise...

AI Technical Summary

Problems solved by technology

Because in the cell culture technique, the virus is adsorbed to the cells to reproduce, the above-mentioned rabies, hemorrhagic fever, Japanese encephalitis, and polio viruses are easily adsorbed on the cells, so the above method is suitable for rabies vaccines, hemorrhagic fever vaccines, The preparation of Japanese encephalitis vaccine, polio vaccine and rotavirus vaccine, and influenza virus is difficult to produce influenza vaccine by the method proposed in the above-mentioned patent without Vero cell adaptation, and this is that so far influenza vaccine still relies on chicken embryo Reasons why large-scale production of influenza vaccines cannot be produced in bioreactors