199 results about "Metabolic waste" patented technology

In a peritoneal dialysis embodiment of the present invention, spent dialysate from the patient's peritoneal cavity passes, along a patient loop, through a dialyzer having a membrane that separates waste components from the spent dialysate, wherein the patient loop returns fresh dialysate to the patient's peritoneal cavity. The waste components are carried away in a second regeneration loop to a regeneration unit or sorbent cartridge, which absorbs the waste components. The regeneration unit removes undesirable components in the dialysate that were removed from the patient loop by the dialyzer, for example, excess water (ultrafiltrate or UF), toxins and metabolic wastes. Desirable components can be added to the dialysate by the system, such as glucose and electrolytes. The additives assist in maintaining the proper osmotic gradients in the patient to perform dialysis and provide the necessary compounds to the patient.

An aseptic watering system for pets is that has a unique filtration and sterilization system for providing a source of drinking water substantially free of toxins, pathogens, resultant metabolic wastes, or other contaminants that may cause bothersome and life threatening diseases. The system also includes means for cooling the drinking water to make it more desirable to a pet.

The invention provides a shrimp-fish-shellfish-alga multi-unit culture and aquatic organism regulation system thereof. A shrimp culture zone, a fish culture zone, a shellfish culture zone, a large scale alga cultivation zone, a probiotics and microalga culture zone, a water processing zone and an emergency drainage system are arranged in a closed water circulation culture system, wherein the culture water circulates in different culture and water processing units. As the water circulates in different culture units and is utilized in multiple levels by various aquatic organisms of different trophic level and ecological niche, the system gives the various aquatic organisms full play to the positive bait resource complementarity, prevents the aquatic organisms from directly competing on living space and dissolved oxygen and reduces the mutual damage caused by organic remains and metabolic waste, achieves the 'self-modification' and 'biomanipulation' of the culture environment, enables the environment itself to recover from the pollution in culture process, and decontaminates the seawater culture environment.

An improved system for large scale production of proteins and / or polypeptides in cell culture is provided. In accordance with the present invention, cells expressing the protein or polypeptide of interest are grown in media that comprise a glycolysis-inhibiting substance. Additionally and / or alternatively, cells expressing the protein or polypeptide of interest are grown in media in which glutamine is limited. The use of such a system allows high levels of protein or polypeptide production and lessens accumulation of undesirable metabolic waste products such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, agricultural or other commercial compositions.

A method of producing a polypeptide in fed batch cell culture is provided which involves an initial cell growth phase and a distinct production phase. In the initial growth stage, animal cells having nucleic acid encoding the polypeptide are cultured at a starting osmolality of about 280-330 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g / L. This is followed by a production phase, where the cultured animal cells of the growth phase are inoculated at a cell seed density of at least 1.0×106 cells / mL and the cells are cultured at a starting osmolarity of about 400-600 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g / L. Preferably, the glutamine concentration in the cell culture medium is simultaneously controlled in order to curtail production of lactic acid and ammonia which result from unnecessarily high glutamine concentrations. During the growth phase, production of potentially detrimental metabolic waste products, such as lactic acid, is controlled thereby curtailing the increase of osmolality due to accumulation and neutralization of waste products. Thus, the cell growth can be improved. In the production phase, the cell culture conditions are modified in order to arrest or reduce cell growth and thereby direct nutrient utilization toward production, as opposed to cell growth. Overall, it is intended that the method results in an improvement in specific productivity, reduction in production run times and / or an increase in final product concentration.

Described herein is a method for reducing levels of the harmful metabolic waste product of S-adenosylmethionine (SAMe), homocysteine, and provide vitamin and other nutritional co-factors that reduce the production of homocysteine and either re-methylate homocysteine back to S-adenosylmethionine, or facilitate its conversion downstream to cystathione. The method of the invention may be achieved by administering 5-methyl tetrahydrofolate, methylcobalamin, and one or more compounds selected from the group consisting of betaine, pyridoxal-5-phosphate, N-acetyl-cysteine, and other cofactors.

A device, system and method for exchanging components between first and second fluids by direct contact in a microfluidic channel. The fluids flow as thin layers in the channel. One of the fluids is passed through a filter upon exiting the channel and is recycled through a secondary processor which changes the fluid's properties. The recycled fluid is reused for further exchange. The filter excludes blood cells from the recycled fluid and prevents or limits clogging of the filter. The secondary processor removes metabolic waste and water by diafiltration.

The invention relates to an isolated organ preserving device and a preserving method thereof, and belongs to the technical field of medical equipment. The isolated organ preserving device is structurally characterized in that continuous mechanical irrigation and membrane oxygenation are adopted, an isolated organ continuously obtains nutrients and the oxygen supply, a cell metabolite is removed in time, and a cell microenvironment is better maintained, so that the function normality is ensured. The isolated organ preserving device disclosed by the invention can be used for directly contacting the organ perfusate and the organ perfusion instrument by adopting a disposable consumable material sterilized through epoxyethane, thereby meeting the requirement for sterility of a system. Besides, the isolated organ preserving device disclosed by the invention can be used for judging the consumption of the nutrients contained in the organ perfusate and the gathering degree of metabolic wastes by monitoring the pH value of the organ perfusate in real time and can be used for abandoning the organ perfusate after a certain standard is achieved and replacing the fresh organ perfusate, thereby further ensuring the possibility of long-time preservation; the isolated organ preserving device can be used for evaluating the damage condition of the isolated organ by monitoring the front and back perfusion pressure of the isolated organ in real time and converting the perfusion resistance by combining a flow number.

A bioreactor hollow fiber perfusion system increases the capacity of standard fed batch bioreactors. The bioreactor hollow fiber perfusion system cycles bioreactor mass through a hollow fiber tangential flow filter which separates the metabolic wastes (as well as proteins) from the biomass material allowing the reactions in the bioreactor to continue when compared to a fed batch bioreactor. The bioreactor hollow fiber perfusion system preferably includes a low shear gamma stable disposable pumphead responsible for biomass re-cycling and can be easy installed or replaced without the risk of contamination.

The invention discloses a method and device for preparing an electrospinning fiber bracket with a three-dimensional structure. The preparation device can be used for generating a body form electric field and directly preparing a three-dimensional electrospinning fiber bracket in the electrostatic spinning process. The preparation method has a simple process and low cost, and is suitable for preparing three-dimensional electrospinning fibers of different chemical strengths, biocompatibilities and degradation properties by using different spinning materials. The three-dimensional electrospinning fiber bracket has the advantages of capability of allowing seed cells enter the bracket smoothly, realization of in-vitro three-dimensional culturing and tissue engineering repair of the seed cells, contribution to the adhesion growth of the cells, conveying of nutrient substances and discharge of metabolic wastes, facilitation of the proliferation, growth and differentiation of the cells in the bracket, increase in the survival rate and breeding speed of the cells, establishment of a good metabolic environment for the growth and proliferation of the cells, contribution to the breeding of the cells and healing of tissues, suitability for cell three-dimensional culturing and large application value in the field of tissue engineering.

The present invention generally relates to electromagnetically-shielded air heating systems and methods. More particularly, the present invention relates to various air heating systems and methods capable of generating heated air and then supplying such heated air directly to a target space, while disposing various sources of such waves away from the target space, thereby minimizing intensities of such waves when measured in the target space. The present invention also relates to various air heating systems and methods for supplying moisture and / or oxygen to the target space along with the heated air, thereby maintaining a humidity and / or oxygen concentration in the target space at or near a preset level, while passively or actively displacing metabolic wastes and / or stale air inside the target space. The present invention further relates to various processes for providing the above air heating systems and various modules and / or members thereof.

The invention discloses a nanofiber bone cartilage repairing stent for tissue engineering and a preparation method thereof. The nanofiber bone cartilage repairing stent for tissue engineering is characterized by comprising a porous cartilage layer, a porous cartilage lower bone layer and a connecting layer between the porous cartilage layer and the porous cartilage lower bone layer, wherein the porous cartilage layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is 20-400mu m in pore diameter; the connecting layer includes a PLLA-based composite degradable polymer material and is of a porous nanofiber structure which is less than 5mu m in pore diameter; and the porous cartilage lower bone layer comprises a PLLA-based composite degradable polymer material containing 5-70% (w / w) of nano-hydroxyapatite and is of a porous nanofiber structure which is 50-500mu m in pore diameter. Microstructure of the bone cartilage repairing stent prepared by the invention is represented as bionic ECM nanofiber; the repairing stent can promote adhesion, propagation and differentiation of related bone cartilage cells on the stent, and can also promote transportation of nutrient substances in the stent and discharge of metabolic waste.

The invention discloses a method for mixed breeding of Sipunculus nudus and Babylonia areolata. At present, Sipunculus nudus breeding is mudflat breeding without feed, suitable mudflats are limited, and the breeding period is as long as 1.5 years. Bait of Babylonia areolata generally comprises chilled trash fishes, residual bait, residues and metabolic waste, water quality decays easily, and large-scale breeding benefit is not high. Through measures including pond construction, breeding pretreatment, seedling breeding, bait feeding, water quality regulation and disease control, complementary mixed breeding of Sipunculus nudus and Babylonia areolata is realized, and the problem that substrate deterioration caused by high-density breeding and bait feeding in the Babylonia areolata breeding process is solved. The Sipunculus nudus ingests various residues produced after decomposition of residual bait, and the growth speed is greatly increased compared with that through sea area natural extensive breeding. With the adoption of the method, the breeding environment of the Babylonia areolata is improved, the breeding survival rate and quality are improved, the breeding period of the Sipunculus nudus is shortened, the breeding space is saved, and the breeding benefit is increased.

The invention discloses a simulation three-dimensional cell cultivation device and a cultivation method. The simulation three-dimensional cell cultivation device highly simulates the in-vivo tumor physiological environment, and can be used for detecting and screening effects of novel anti-tumor medicines or detecting sensitivity of tumor cells on different anti-tumor medicines, factors such as nutrition supply of the tumor cells, removal of metabolic wastes, blood vessels and vascular endothelial cells are considered, and the in-vivo tumor physiological environment is close to the in-vivo tumor growth environment. By the invention, the in-vitro tumor cell physiological environment is greatly close to the in-vivo environment; and by the new technology, detection and screening of the novel anti-tumor medicines and tumor cell medicine sensitive tests are improved to a new height.

The invention discloses an environment-friendly and high-yield breeding method for penaeus vannamei boone. The method is characterized by comprising the steps of previous-breeding preparation, culture of chaetoceros, adding of a carbon source, feeding and management, water quality monitoring and the like; before breeding, favorable chaetoceros is added to occupy an ecological niche; during breeding, the input ratio of feed to glucose is regulated, heterotrophic bacteria can be stimulated to absorb nitrogen and phosphorus metabolic waste for mass propagation, and then the function of water purification is achieved; by the adoption of a special siphon dirt collection device with an excellent dirt discharge effect, the method enables the water change frequency to be reduced to one time every seven days, and the water change volume is only 5%. The method has the advantages of being simple and controllable, remarkably reducing the feed conversion rate and water change cost and greatly increasing the yield and survival rate of the penaeus vannamei boone, thereby having good biological and economic benefits.

The invention relates to a 3D printing device and method of a multi-scale fiber-reinforced micro-channel active tubular tissue, wherein the device comprises a printing device shell, a printing platform is arranged in the printing device shell, the printing platform can move along the X-Y direction, a receiving roller is connected with the printing platform, the receiving roller is connected with amotor, a coaxial spray nozzle and a melting electrostatic printing device are arranged above the receiving roller, the coaxial spray nozzle and the melting electrostatic printing device are both fixed on a Z-axis moving platform, an inner spray nozzle and an outer spray nozzle of the coaxial spray nozzle are connected with a precise injector respectively. Through the combination of the melting electrostatic printing technology, melt extrusion molding technology and cell printing technology, the on-demand adjustment of three-dimensional tubular tissue mechanical properties can be realized, microchannels are formed in the annular wall of the tubular tissue, nutrients and metabolic wastes are transferred to cells in the tissue in time, the living environment with densely covered fibers in the body can be simulated more realistically, and the expression of cell function is facilitated.

The invention relates to a preparation method of loquat distilled liquor. The preparation method comprises the following steps: (1) cleaning a fresh loquat fruit, removing a pit, and beating to obtain loquat slurry; (2) adding saccharose which accounts for 10wt%-12wt% of the loquat slurry, and uniformly stirring in time; (3) adding pectinase which accounts for 0.01wt%-0.015wt% of the loquat slurry, and uniformly stirring in time; (4) inoculating a self-made fresh loquat bran saccharomycete which accounts for 0.4wt%-0.6wt% of the loquat slurry, and uniformly stirring in time, wherein the fresh loquat bran saccharomycete is prepared from saccharomyces cerevisiae 2.145 (CGMCC NO.9753); (5) carrying out closed anaerobic fermentation on the loquat slurry at 28-30 DEG C for about 25-30 days to obtain fermentation liquor; and (6) distilling the fermentation liquor to obtain the distilled white liquor. The loquat distilled liquor produced through the preparation method disclosed by the invention is strong in loquat fruit flavor and mellow in liquor body and can be used for effectively reducing the influence of a fermenting metabolic waste on the product quality.

The invention relates to slowly-sinking puffed compound feed for misgurnus anguillicaudatus and a production process thereof, and belongs to the technical field of misgurnus anguillicaudatus feed. The feed comprises 5-10 parts of fish meal, 3-10 parts of pork meal, 10-25 parts of bean meal, 3-8 parts of peanut meal, 5-15 parts of cottonseed meal, 5-20 parts of rapeseed meal, 3-10 parts of corn DDGS, 5-15 parts of rice bran, 15-20 parts of flour, 2-4 parts of soybean oil, 1-3 parts of monocalcium phosphate, 2-5 parts of bentonite, 0.2-1.5 parts of vitamin premix, 1-3 parts of microelement premix, 0.05-0.5 part of choline chloride, 0.05-0.1 part of antioxidant and 0.05-0.1 part of mould inhibitor. The utilization rate of the slowly-sinking puffed compound feed is increased for the misgurnus anguillicaudatus, healthy growth of the misgurnus anguillicaudatus is promoted, and pollution of remnant feed and metabolic waste to aquatic water is reduced. The volume weight of the produced slowly-sinking puffed compound feed is kept between that of granular feed and that of puffed feed, the feed has the characteristic of sinking slowly in water like the granular feed and further has the advantage of being long in water resisting time like the puffed feed, and therefore the feed meets the feeding characteristics of misgurnus anguillicaudatus.

The present invention discloses a method of preparing enzymes in a biomimetic manner and the enzymes capable of eliminating atherosclerosis. A first contribution is as follows: cognitive limitations of probiotics and enzymes in the existing technology are broken through, plant own endophytes and endogenous enzymes are used in the biomimetic manner to produce the enzymes, and active ingredients invarious plants are fully released; a second contribution is as follows: in classifications of plant phyla, classes, orders, families, tribes, genera and species, due to the close endophytes and endogenous enzymes, the plants within the same families can be constrainedly fermented together, or plant characteristic components in the fermentation processes are seriously affected; a third contributionis as follows: based on academic points of eliminating pathogens to strengthen vital qi in ''Confucians' Duties to Their Parents'' and macroscopic thoughts that all the pathogen eliminating methods can be achieved by three methods of sweating, emesis and purgation, food enzymes with functions of the sweating, emesis and purgation are used to replace medicines, and purposes of clearing body metabolic waste are achieved by the three ways of the sweating, emesis and purgation; and a fourth contribution is as follows: the method merges modern technology, reduces production difficulties, shortensproduction cycles, and improves product quality.

The invention provides a preparation method of a nerve conduit stent. The method comprises the following steps that materials are dissolved in a polar solvent to obtain a dissolution solution; the materials are selected one or several materials from polylactic acid-polyglycolic acid copolymers, polyethylene carbonate, polylactic acid and polyglycolic acid; the dissolution solution is coated or flatly paved on a mold to obtain a mold with the dissolution solution; the mold with the dissolution solution is put into water to obtain a tubular stent. The materials such as biodegradable polylactic acid-polyglycolic acid copolymers PLGA, polypropylene carbonate PPC and the like are used; a nerve conduit is prepared by a simple and easy-to-operate solution replacement method; high support intensity and tensile strength are realized; feasible microenvironment can be provided for the nerve regeneration; the pore connectivity is high; the exchange transportation of nutrient substances and metabolic wastes is facilitated; the biocompatibility is good; the adhesion growth of nerve cells and neurogliocytes is facilitated; the nerve regeneration is promoted; the complete degradation is realized;no side effect is generated on the body.

The invention provides a functional traditional Chinese medicine pillow. The pillow is prepared from, by weight, 10-50 parts of tea, 10-30 parts of folium artemisiae argyi, 10-50 parts of lotus leaves, 10-30 parts of folium mori, 5-15 parts of salvia miltiorrhiza, 5-15 parts of ginseng, 30-90 parts of semen cassiae, 5-15 parts of radix bupleuri, 1-33 parts of asarum sieboldii, 5-15 parts of angelica dahurica, 10-30 parts of radix puerariae, 5-15 parts of rhizoma chuanxiong, 10-30 parts of ligusticum sinense oliver, 10-30 parts of chrysanthemum morifolium, 10-30 parts of notopterygium incisum, 10-50 parts of prunella vulgaris, 5-15 parts of mint and 10-30 parts of tuber fleeceflower stems. The functional pillow is capable of improving blood and oxygen supply of the head, deepening sleep, promoting smooth blood circulation, eliminating brain cell metabolic waste, clearing brain fatigue, improving insomnia and festless sleep, relieving dizziness and headaches, improving microcirculation, promoting circulation, removing stasis, tranquilizing and allaying excitement and recovering self-adjusting function of the central nervous system.

A coated smooth surface of a substrate repels liquids and viscoelastic materials and can have anti-staining and anti-biofouling properties. The smooth surface can be coated by applying a chemical layer on the surface and a lubricant. Such coated surfaces are useful for use in toilets, urinals, or other devices for the processing of liquids and viscoelastic materials such as solid or semi-solid metabolic waste of human digestive system. Such coated surfaces can also be applied to windows for buildings or vehicles such as automobiles or camera lenses to repel liquids (e.g., rain), ice, frost, insect residues, and birds' feces.

The invention relates to a bionic artificial bone scaffold and a preparation method thereof. The method comprises the following steps: preparing material solutions A and B; utilizing an electrostatic spinning method to respectively prepare the material solutions A and B into a central channel layer and a peripheral column layer of a scaffold, wherein nanometer fibers in the central channel layer are in same spiral directions and the fibers in two adjacent nanometer fiber layers in the peripheral column layer are in opposite spiral directions; micro-level finely simulating a three-dimensional microenvironment of an osteon; hierarchically adding growth factors, supplying a differential inducing environment to seed cells BMSCs and constructing the bionic artificial bone scaffold with vascularizing tissues in the central channel layer and bone tissues in the peripheral column layer. The staggered orientation of the nanometer fibers in the scaffold can greatly enhance the structural stability; effective mechanic support is supplied to a bone defect part; the mutually connected network frame thereof is beneficial to cell permeation growth and blood vessel and nerve growth and also is convenient to transfer nutrient substances and metabolic wastes; the bionic artificial bone scaffold has ideal popularization and application values in the fields of biomedical materials and tissue engineering.

A method for preparing artificial vessels, disclosed by the invention, comprises preparing a template by 3D printing; preparing an active polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (active POSS-PCU); mixing the active POSS-PCU with stem cells to form the artificial vessels by 3D printing followed by plasma processing; removing the template to form the artificial blood vessels with an access. The method provides to prepare the artificial vessels with three-layer structures, which are capable of transporting nutrients and oxygen, removing metabolic wastes and enhancing haemocompatibility and biological stability. Therefore, the method for preparing the artificial vessels solves the problem of angiemphraxis caused by the artificial vessels with single-layer structure.

The culture chamber of the present invention has a fluid-filled culture compartment in which cells, tissues and other biologicals are cultured. The culture compartment is transversed by one or more molecular weight cut-off membranes attached to a membrane carrier assembly. Incoming nutrients are transported through the membrane into the culture compartment and metabolic waste products are transported away from the fluid-filled culture compartment through the membrane and out the chamber outlet. Both reusable and disposable culture chambers are described for culturing cells, cell aggregates, particles, tissues and organoids.

The invention relates to a sturgeon cultivation method, in particular to a cultivation method capable of increasing the survival rate of sturgeon fries. The invention aims to overcome the defects that the survival rate of the sturgeon fries is low in the conventional cultivation technology and the cultivation method capable of increasing the survival rate of the sturgeon fries is not provided. The cultivation method capable of increasing the survival rate of the sturgeon fries comprises a mouth-opening culture period and a habituated culture period, wherein the sturgeon fries are auxiliarily fed with mouth-opening nutritional baits within 15 to 20 days after the sturgeon fries open mouths and eat food; the mouth-opening nutritional baits is prepared from the following components in percentage by weight: 10 to 30 percent of maltodextrin, 25 to 50 percent of protein powder, 10 to 30 percent of yeast extract powder, 8 to 12 percent of mannoprotein, 0.5 to 1 percent of selenium yeast, 0.5 to 1 percent of lysine and 0.5 to 1.0 percent of tryptophan. The cultivation method capable of increasing the survival rate of the sturgeon fries is simple in operation and low in cost; the fed mouth-opening nutritional baits have rich and balanced nutrition and high palatability and attractiveness; the sturgeon fries have high utilization ratio and few metabolic wastes; and the survival rate of the sturgeon fries at the mouth-opening culture period can be increased obviously.

The invention relates to compound feed for schizothorax prenanti, and belongs to the technical field of fish feed. The compound feed for the schizothorax prenanti comprises 30-48 parts of imported fish meal, 10-20 parts of soybean meal, 5-10 parts of cotton seed mill, 5-10 parts of rapeseed meal, 10-15 parts of flour, 5-10 parts of beer yeast, 2-5 parts of squid extract, 5-8 parts of wheat gluten, 4-6 parts of rice bran, 2-5 parts of fish oil, 2-5 parts of soya-bean oil, 2-5 parts of soybean lecithin oil, 0.3-0.5 part of phagostimulant, 0.2-0.5 part of vitamin premix, 1-3 parts of trace mineral premix, 1-3 parts of monocalcium phosphate, 0.1-0.3 part of salt, 0.03-0.06 part of a mildew-retarding agent, 0.1-0.5 part of choline chloride, 0.4-0.8 part of vitamin C acetate, 0.02-0.05 part of vitamin E and 0.01-0.05 part of phytase. According to the compound feed for the schizothorax prenanti, growth requirements of the schizothorax prenanti can be met, the utilization rate of the feed for the schizothorax prenanti is improved, and pollution of cultural water caused by nitrogen and phosphorus in metabolic waste is reduced; healthy and rapid growth of the schizothorax prenanti is facilitated, immunity and premunition of the schizothorax prenanti can be enhanced, and a disease risk of schizothorax prenanti culture is decreased.

The invention relates to a Chinese medicinal formula for treating chronic renal failure. The formula consists of the following raw materials by weight (counted by gram): 12-16g of roasted aconite (decocted firstly), 12-16g of south dodder seed, 15-22g of cortex eucommiae, 15-22g of astragalus, 15-22g of dangshen, 15-22g of indian buead, 12-18g of large-head atractylodes rhizome, 15-22g of Chinese yam, 12-18g of pokeberry root, 12-16g of areca peel, 15-22g of umbellate pore fungus, 20-30g of rhubarb, 25-32g of red-rooted salvia root, 25-32g of honeysuckle flower, 25-32g of dandelion and 15-22g of sweet basil. After being taken by a patient suffering from chronic renal failure, a medicament prepared in the weight ratio has the effects of exciting potential, improving physique, improving symptoms, promoting the excretion of metabolic waste, relieving the state of an illness and prolonging life.

The invention provides a microvalve-based dual-detection microfluidic chip and a method for constructing a diabetes model. The chip is composed of three layers of PDMS, the upper layer is the liquid circuit layer, the lower layer is the air circuit control layer, and the middle of the upper and lower layers is the PDMS membrane. A method for constructing a diabetes model based on a microvalve-based dual-detection microfluidic chip. A single nematode is added to the entrance of the worm and the drug, and the single worm is introduced into the nematode fixation and culture room. During fluorescence imaging, the air pressure is used to deform the PDMS membrane, and the worm body is compressed. It is fixed and imaged on the side wall of the sampling channel; material exchange is performed three times a day to add fresh food and apply glucose or drug stimulation to discharge nematode metabolic waste. The invention adopts channel design, which is simple to operate and easy to realize material exchange, which is beneficial to the long-term cultivation of nematodes; the method of fixing nematodes adopts the method of membrane deformation, which is safe and harmless to the nematodes, and can be used for long-term cultivation, movement and imaging of a single nematode. detection.