The invention discloses a preparation method and application of a bone repair material having a multi-dimensional channel structure. The bone repair material is formed by stacking at least two laminated substrate structure layers, wherein each of the substrate structures is provided with through holes and grooves, and each of the substrate structures is prepared through substrate material preparation, substrate prefabricating, filler preparation, filling, stacking, forming, heat processing and heat treatment. According to the bone repair material prepared by the invention, three-dimensional network channels are constructed by the through holes and the grooves of the substrate structures and the channels are filled with collagen fibers, so that the bone repair material is conducive to cell adhesion growth and the in-growth of blood vessels and nerves, and the bone repair material is also convenient for transfer of nutrient substances and excretion of cell metabolites; meanwhile, cells are uniformly distributed inside the material, which is conducive to the formation of uniform and consistent tissues inside a stent; and the structural material can achieve firm biological chimerism with new bone tissues and can keep a good structural stability during degrading.