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Lactobacillus gasseri with hypoglycemic capacity and application of lactobacillus gasseri

A Lactobacillus gasseri, hypoglycemic technology, applied in the field of microorganisms, can solve the problem of not having the function of inhibiting dipeptidyl peptidase

Pending Publication Date: 2022-04-08
GREENS BIOENG SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this strain does not have the effect of inhibiting dipeptidyl peptidase-4 and α-glucosidase

Method used

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  • Lactobacillus gasseri with hypoglycemic capacity and application of lactobacillus gasseri
  • Lactobacillus gasseri with hypoglycemic capacity and application of lactobacillus gasseri
  • Lactobacillus gasseri with hypoglycemic capacity and application of lactobacillus gasseri

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: Isolation and identification of Lactobacillus gasseri

[0051] 1. Sample collection

[0052] The applicant team collected samples of self-made fermented goat milk from herdsmen in Xinjiang. During the sampling process, gloves were worn and disinfection was performed to avoid contact with samples to prevent contamination. Put the sample into a sterile centrifuge tube and seal it, and immediately refrigerate it.

[0053] 2. Strain Isolation

[0054] After the ten-fold gradient dilution of the fermented goat milk sample, select appropriate multiple gradient dilutions and spread them evenly on the MRS medium solid plate, and place the plate upside down in a 37°C incubator for anaerobic cultivation for 48 hours; Select a single colony from the culture plate for transfer culture. Finally, a strain with good growth performance was obtained, which was named CKCC 1913.

[0055] 3. Identification of strains

[0056] The bacterium colony of the bacterial strain ...

Embodiment 2

[0061] Example 2: Tolerance of strain CKCC 1913 to simulated gastric juice

[0062] Take the culture solution of Lactobacillus gasseri strain CKCC 1913 cultured for 18 hours, collect the bacteria by centrifugation at 8000r / min, 4°C, 10min, wash with sterile saline solution for 2 to 3 times, collect the bacteria by centrifugation, and resuspend in pH 2.5 In simulated gastric juice, the blank control group was resuspended in sterile saline. Cultivate at 37°C for 2 hours, and calculate the survival rate of lactic acid bacteria at 0 hour (just take the blank control group), 0.5 hour, 1 hour, 1.5 hour, and 2 hour respectively. The survival rate calculation formula is as follows:

[0063] Survival rate (%)=[log CFU Nt / log CFU Nc]×100%

[0064] Among them, Nt: the number of lactic acid bacteria after treatment; Nc: the number of lactic acid bacteria in the 0h blank control group.

[0065] Results: The survival rate of strain CKCC 1913 incubated in simulated gastric juice at pH 2.5...

Embodiment 3

[0066] Example 3: Tolerance ability of strain CKCC 1913 to simulated intestinal fluid

[0067] Take the culture solution of Lactobacillus gasseri strain CKCC 1913 cultured for 18 hours and collect the bacteria by centrifugation at 8000r / min, 4°C for 10 minutes, wash with sterile saline solution for 2 to 3 times, collect the bacteria by centrifugation, and resuspend in 0.3% Bile salt, pH 8 simulated intestinal juice, blank control group resuspended in sterile saline. After incubation for 6 hours in a constant temperature incubator at 37°C, the survival rate of lactic acid bacteria was counted. The survival rate calculation formula is the same as the above method.

[0068] Results: The survival rate of strain CKCC 1913 was 42.44% after incubation for 6 hours in simulated intestinal fluid containing 0.3% bile salt and pH 8.

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Abstract

The invention relates to a Lactobacillus gasseri strain CKCC 1913 and application thereof, the strain is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on August 23, 2021, and the preservation number is CGMCC No.23175. The Lactobacillus gasseri strain CKCC 1913 has the advantages that the Lactobacillus gasseri strain CKCC 1913 can be used for preparing the lactobacillus gasseri strain CKCC 1913; a bacterial suspension, a cell metabolite and a cell content of the lactobacillus gasseri CKCC 1913 have a very good inhibition effect on the activity of dipeptidyl peptidase-4 and alpha-glucosidase, have antioxidant activity and can be used for preparing hypoglycemic drugs and antioxidant products. In addition, the strain CKCC 1913 has high gastrointestinal fluid tolerance and good gastric mucosa protein adhesion capacity, the viable count of CKCC 1913 single-strain fermented milk in the 21-day storage period is basically kept unchanged, and the acidity change is lower than 20 DEG T. Comprehensive research results show that the strain CKCC 1913 can be used as probiotics for reducing blood sugar and increasing the viable count of fermented milk products in the shelf life, and has huge application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Lactobacillus gasseri strain CKCC 1913 and an application thereof. Background technique [0002] Blood sugar mainly refers to the glucose in the blood. Hyperglycemia is a state in which blood glucose levels exceed normal values ​​on an empty stomach or 2 hours after a meal. Chronic hyperglycemia develops to a certain extent and will evolve into diabetes. China has become the country with the largest number of diabetic patients in the world, with more than 100 million diabetic patients. Therefore, effective measures are needed to prevent and treat diabetes. Dipeptidyl peptidase-4 (dipeptidyl peptidase IV, DPP-IV) inhibitors are the latest and most promising antidiabetic drugs. In 2009, the China Food and Drug Administration approved the first DPP-IV inhibitor, sitagliptin, to be marketed in China. These oral DPP-IV inhibitors exert beneficial effects in blo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A61K35/747A61P3/10A61P39/06C12R1/225
Inventor 刘爱洁骆鹏飞
Owner GREENS BIOENG SHENZHEN
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