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Preparation method of corydalis ochotensis total alkaloid

A technology of total alkaloids and alkaloids, applied in the direction of medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve the problems of no specific treatment, etc., achieve abundant resources, improve learning and memory functions, and have a large reserve Effect

Inactive Publication Date: 2012-07-04
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no treatments that specifically treat or reverse disease progression

Method used

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  • Preparation method of corydalis ochotensis total alkaloid
  • Preparation method of corydalis ochotensis total alkaloid
  • Preparation method of corydalis ochotensis total alkaloid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] a. Corydalis chinensis is crushed into coarse powder, particle size 0.1mm, add 3 times the amount of 0.1% hydrochloric acid solution and soak for 1 hour,

[0018] b. Use 10 times of 0.1% hydrochloric acid solution for percolation extraction until the percolation liquid has no alkaloid reaction,

[0019] c. The percolation solution is adjusted to pH 5 with KOH solution, put on the macroporous resin column AB-8, eluted with 1% KOH solution until the effluent is nearly colorless, then washed with water until the pH value is 8, and washed with 40% After elution with ethanol until the effluent is nearly colorless, elute with 80% ethanol until there is no alkaloid reaction, recover ethanol from the eluent, and dry it.

Embodiment 2

[0021] a. Corydalis corydalis is crushed into coarse powder, particle size 10mm, add 6 times the amount of 2.5% sulfuric acid solution and soak for 12 hours,

[0022] b. Use 15 times of 2.5% sulfuric acid solution for percolation extraction until the percolation liquid has no alkaloid reaction,

[0023] c. The percolation solution is adjusted to pH 7 with NaOH solution, put on macroporous resin column D101, eluted with 5% NaOH solution until the effluent is nearly colorless, then washed with water until the pH value is 7, and washed with 40% ethanol After stripping until the effluent is nearly colorless, it is eluted with 90% ethanol until there is no alkaloid reaction, and the eluate is recovered with ethanol and dried.

Embodiment 3

[0025] a. Corydalis japonica is crushed into coarse powder, particle size 20mm, add 10 times the amount of 5% phosphoric acid solution and soak for 24 hours,

[0026] b. Use 20 times of 5% phosphoric acid solution for percolation extraction until the percolation liquid has no alkaloid reaction,

[0027] c. NH for percolation 4 The OH solution was adjusted to a pH value of 8, applied to a macroporous resin column DA201, eluted with 10% NaOH solution until the effluent was nearly colorless, then washed with water until the pH value was 9, and eluted with 40% ethanol until the effluent was almost colorless. After coloring, elute with 95% ethanol until there is no alkaloid reaction, ethanol is recovered from the eluent, and dried.

[0028] Further verify the effect of medicine of the present invention below in conjunction with pharmacodynamics experiment.

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Abstract

The invention relates to a corydalis ochotensis total alkaloid and a preparation method and a medicinal application thereof. The corydalis ochotensis total alkaloid is prepared by the following methods: grinding corydalis ochotensis into coarse powder; adding sulfuric acid solution with a concentration of 0.1-5% for soaking the coarse powder; then carrying out extraction by the sulfuric acid solution with a concentration of 0.1-5% by means of percolation; adjusting pH value of percolation liquid to 5-8 by NaOH solution; applying macroporous resin, eluting the percolation liquid by NaOH solution with a concentration of 1-10% until effluent liquid becomes almost colorless; washing the percolation liquid until the pH value of the percolation liquid ranges from 7 to 9; eluting the percolationliquid by ethanol with a concentration of 40% until the effluent liquid becomes colorless; eluting the percolation liquid by ethanol with a concentration of 80-95% until no alkaloid reactions occurs;and finally recovering ethanol by eluent and drying.The invention also relates to the application of the corydalis ochotensis total alkaloid in preparing medicine for improving memory function and treating senile dementia.Being a common medicinal material in Changbaishan Mount in China, the corydalis ochotensis is in existence in large quantities; therefore development of such a medicinal material is of positive significance.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine. Background technique [0002] There are about 350 species of Corydalis in the Papaaceae family, distributed in the northern temperate zone. There are about 290 species in our country, which are produced in all parts of the country, mostly in the southwest. Corydalis has a long history of treating diseases. Traditional Chinese medicine is mostly used to clear away heat and detoxify, dehumidify and relieve pain, and treat colds, fever, sores, carbuncles, and ulcers; Tibetan medicine is used to treat colds and fevers, hepatitis, edema, gastritis, cholecystitis, and high blood pressure. and many other diseases. Judging from the current research results on the chemical components of this genus of plants, this genus of plants mainly contains isoquinoline alkaloids, and most of the plants of this genus and their extracts have a wide range of pharmacological activities, such as regulating th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/66A61P25/28
Inventor 赵春芳
Owner JILIN UNIV
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