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Method for analyzing influence of multi-dimensional nano material on cell metabonomics

A technology of nanomaterials and cell metabolism, applied in the field of analysis of multidimensional nanomaterials affecting cell metabolomics, to achieve the effects of environmental protection of materials, improvement of test efficiency, and convenient operation

Pending Publication Date: 2020-10-09
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology

However, there are few reports on the combination of metabolomics analysis platform and the study of biological properties of nanomaterials in different dimensions.

Method used

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  • Method for analyzing influence of multi-dimensional nano material on cell metabonomics
  • Method for analyzing influence of multi-dimensional nano material on cell metabonomics
  • Method for analyzing influence of multi-dimensional nano material on cell metabonomics

Examples

Experimental program
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Embodiment 1

[0051] Example 1 Analysis Method of Multidimensional Carbon Nanomaterials Affecting Cell Metabolomics

[0052] Multi-dimensional carbon nanomaterials: zero-dimensional material fullerene, one-dimensional material carbon nanotube, two-dimensional material graphene, all purchased from Nanjing Xianfeng Nano Material Technology Co., Ltd.

[0053] Medium: DMEM (Dulbecco Modified Eagle Medium) medium containing 10% fetal bovine serum and 1% penicillin-streptomycin solution.

[0054] The specific steps of the metabolomics analysis method are as follows:

[0055] 1. "Multidimensional nanomaterial-cell" co-culture

[0056] Place the culture dish seeded with A549 cells in 5% CO 2 cultured in an incubator at 37°C in an atmosphere. Change the medium every two days to maintain cell viability.

[0057] After the cells grow to the exponential growth phase, the cells are washed with phosphate buffer, and then 1 ml of trypsin is added to obtain a cell suspension. Take 2ml cell suspension ...

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Abstract

The invention relates to a method for analyzing influence of a multi-dimensional nano material on cell metabonomics. The analysis method for the influence of the multi-dimensional nano material on thecell metabonomics based on the liquid chromatography-mass spectrometry technology comprises the following steps: (1) co-culturing the multi-dimensional nano material and cells; (2) performing metabolite extraction; (3) carrying out liquid chromatography-mass spectrometry combined detection; and (4) performing bioinformatics statistical analysis. According to the metabonomics analysis method, theinteraction between the multi-dimensional nano material and cells is investigated, the cell metabolite is detected and analyzed, and the cell metabolite variety contents and the metabolic pathways under the effects of different multi-dimensional nanometer materials are compared so that the influence principle of the nanometer materials with different dimensions on the cell metabonomics is revealed, the basis is provided for the evaluation of the biological characteristics of the nanometer materials, and the application prospect is broad.

Description

technical field [0001] The invention relates to the field of metabolomics analysis, in particular to an analysis method for multidimensional nanomaterials affecting cell metabolomics. Background technique [0002] As a classic detection and analysis technology with many unique features and significant advantages, metabolomics analysis and its related principles and applications have attracted great attention. At the same time, research on the further innovation and development of this technology has also It has been going on for many years and it has achieved great results. By using modern systems biology research methods (including instruments and software) to detect and analyze thousands of small molecules (<1500Da), metabolomics analysis has successfully revealed and explained the structure, content and relationship between various metabolites and organisms. inherent connection. Due to its attractive features (such as biocompatibility with phenotype, high-throughput ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86G01N33/50
CPCG01N30/02G01N30/06G01N30/7253G01N30/8696G01N33/5038C12Q1/02
Inventor 张定坤龚萌
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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