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Preparation method of composite microecological preparation containing fermentation medium

It is a technology of compound microecology and culture medium, which is applied in the molding or processing of animal feed, animal feed, and additional food elements. , the effect of stimulating growth

Inactive Publication Date: 2019-03-29
吕锋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although the prior art discloses a series of probiotics containing bacteria, they are mainly living bacteria preparations with a short storage period, which are mainly used to promote the proliferation of probiotics in the intestinal tract of animals, and the effect is single

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of complex saccharification solution

[0062] Dissolve 12kg of ultra-finely pulverized corn flour and soybean flour with a mass ratio of 5:1 in 48L of water in a heating kettle with stirring, soak for 120 minutes at 55°C, heat up to 80°C, stir for 100 minutes, and double-layer gauze Filtrate while hot, boil the filtrate for 15 minutes for saccharification after filtration, cool to room temperature, add betaine with a mass ratio of 0.4%, to obtain a complex saccharification liquid, which is ready for use.

[0063] In the following examples, the yeast is Saccharomyces cerevisiae S288C, and the article number is 204508D-5 TM , purchased from ATCC; the L-valine producing bacterium is Brevibacterium flavum L-valine, purchased from ATCC, the article number is 21129 TM .

Embodiment 2

[0065] Preparation of compound probiotics

[0066] (1) Betaine-induced culture of yeast seed liquid and Brevibacterium flavum seed liquid:

[0067] S1: Preparation of Saccharomyces cerevisiae seed liquid medium (1L): Take 100ml of compound saccharification solution, 30g of glucose, 15g of peptone, 5g of yeast powder, 3g of glycerol, 1g of ammonium sulfate, 1.5g of dipotassium hydrogen phosphate, 2g of sodium chloride, sulfuric acid Magnesium 0.5g, manganese sulfate 0.05g, betaine 3g, adjust the pH value to 6.2, add distilled water to make up to 1000ml, after heating to dissolve, sterilize by high-pressure steam at 121°C for 20min.

[0068] Primary seed culture of Saccharomyces cerevisiae: prepare 1×10 strains of Saccharomyces cerevisiae 8 The strain suspension of CFU / ml was inoculated into a 500ml Erlenmeyer flask containing the seed medium according to the inoculum amount of 5%, and cultured on a shaking table at 30°C and 200r / min for 24h to obtain a first-grade seed solutio...

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PUM

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Abstract

The invention provides a preparation method of a composite microecological preparation containing a fermentation medium. The composite microecological preparation is prepared by the following steps: yeast and L-valine-producing bacteria are subjected to combined fermentation culture in a solid medium containing betaine, and then subjected to bacteriolysis treatment and a self-chelating reaction. The composite microecological preparation contains cell metabolites such as cell contents, betaine and L-valine. The composite microecological preparation of the invention is rich in bacterial cell wall fragments, multivitamins, minerals, small peptides, organic acids, vitamins, nucleotides, oligosaccharides, polysaccharides and various growth-promoting factors, and the like, and can effectively improve the utilization rate of a feed to animals.

Description

technical field [0001] The invention belongs to the field of solid probiotics, in particular to a compound probiotics based on fermentation medium. Background technique [0002] As a new type of non-toxic and non-polluting microbial product feed additive, microecological preparations can regulate the microecological balance of animals, prevent diseases, improve feed conversion rates, improve animal production performance and protect the ecological environment. substitute role. In the prior art, the probiotic preparations are usually based on live bacteria preparations, and a small part uses the contents of the crushed bacteria as the main component. The latter has a more efficient digestion and absorption rate and richer nutritional components, and is receiving more and more attention. [0003] However, this kind of preparation is usually prepared from a single bacterium, the extracellular metabolites of the bacterium are relatively messy, the content of beneficial compone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/105A23K10/12A23K10/14A23K40/10A23K10/30A23K10/37A23K50/75
CPCA23K10/12A23K10/14A23K10/30A23K10/37A23K20/105A23K40/10A23K50/75Y02P60/87
Inventor 吕锋张集汇
Owner 吕锋
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