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Preparation method of super-hydrophilic cell growth surface

A growth surface, super-hydrophilic technology, applied in the field of cell culture devices, can solve the problems of unfavorable stem cell adherent growth, poor hydrophilicity, etc., and achieve stable super-hydrophilic surface modification effect, unchanged performance, and high transparency. Effect

Inactive Publication Date: 2015-07-22
GUANGZHOU JET BIOFILTRATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The cell culture surface made of polystyrene is non-polar and has poor hydrophilicity, which is not conducive to the adherent growth of stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing a superhydrophilic cell growth surface, comprising the following steps in sequence:

[0016] 1) Pretreatment: Ar and O 2 The mixed gas is passed into the plasma reaction chamber, under the conditions of vacuum degree of 20mT, temperature of 30°, gas flow rate of 10sccm, processing power of 350w, and processing time of 1100s, the polystyrene base layer is subjected to plasma etching treatment, so that The surface of the polystyrene base layer forms a concave-convex structure;

[0017] 2) Grafting modification treatment: apply acrylic acid monomer to the surface of the polystyrene base layer after step 1) pretreatment in the form of spray, under the condition of vacuum degree of 20mT, temperature of 30°, gas flow rate of 20sccm, treatment power Under the conditions of 50w and a treatment time of 1200s, the surface of the polystyrene base layer was grafted with acrylic monomers to form a super-hydrophilic cell growth surface.

[0018] The finished ...

Embodiment 2

[0020] A method for preparing a superhydrophilic cell growth surface, comprising the following steps in sequence:

[0021] 1) Pretreatment: Ar and O 2 The mixed gas is passed into the plasma reaction chamber, and under the conditions of vacuum degree of 50mT, temperature of 30°, gas flow rate of 5sccm, processing power of 60w, and processing time of 100s, the polystyrene base layer is subjected to plasma etching treatment, so that The surface of the polystyrene base layer forms a concave-convex structure;

[0022] 2) Grafting modification treatment: apply acrylic acid monomer to the surface of the polystyrene base layer after step 1) pretreatment in the form of spraying, in a vacuum of 20T, a temperature of 30°, a gas flow rate of 20sccm, and a treatment power Under the condition of 60w and treatment time of 100s, the surface of the polystyrene base layer was grafted with acrylic monomer to form a super-hydrophilic cell growth surface.

[0023] The finished product described...

Embodiment 3

[0025] A method for preparing a superhydrophilic cell growth surface, comprising the following steps in sequence:

[0026] 1) Pretreatment: Ar and O 2 The mixed gas is passed into the plasma reaction chamber, and under the conditions of vacuum degree of 30mT, temperature of 35°, gas flow rate of 20sccm, processing power of 80w, and processing time of 80s, the polystyrene base layer is subjected to plasma etching treatment, so that The surface of the polystyrene base layer forms a concave-convex structure;

[0027] 2) Grafting modification treatment: apply acrylic acid monomer to the surface of the polystyrene base layer after step 1) pretreatment in the form of spraying, in a vacuum of 50mT, a temperature of 35°, a gas flow rate of 80sccm, and a treatment power Under the conditions of 120w and a treatment time of 500s, the surface of the polystyrene base layer was grafted with acrylic monomers to form a super-hydrophilic cell growth surface.

[0028] The finished product des...

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Abstract

The invention discloses a preparation method of a super-hydrophilic cell growth surface. The preparation method sequentially comprises the following steps: 1) preprocessing, to be specific, mixed gases of Ar and O2 are introduced into a plasma reaction chamber, a polystyrene substrate is subjected to plasma etching treatment; 2) an acrylic monomer is applied on the surface of the preprocessed polystyrene substrate in a spraying manner, so that the surface of the polystyrene substrate is grafted with the acrylic monomer, and the hydrophilic cell growth surface is formed. The super-hydrophilic cell growth surface has a lasting stable super-hydrophilic effect, and the super-hydrophilic effect is measured by a contact angle meter to be less than 10 degrees. Besides, the material physical property of a polystyrene plastic is not changed, the biological property for cell culture is met, and the high transparency of the polystyrene substrate is guaranteed.

Description

technical field [0001] The invention relates to a cell culture device, in particular to a method for preparing a super-hydrophilic cell growth surface. Background technique [0002] With the continuous development of modern science, especially the rapid progress of biomedicine, stem cell culture has become a very important basic work in many research and application fields. For a long time, people have generally used polystyrene culture plates or culture bottles as tools for cell culture. Polystyrene is a colorless and transparent thermoplastic, usually non-toxic, odorless, colorless and transparent particles, glass-like brittle material, the general formula is [(CH 2 CHC 6 h 5 )n], the English name is Polystyrene, referred to as PS. Its products have excellent water resistance and moisture resistance. In people's production and life, polystyrene has a wide range of uses, and cell culture is one of the major uses. [0003] The cell culture surface made of polystyrene i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/16C08J7/12C08F257/02C08F220/06C08L25/06
Inventor 袁建华袁晔方想元
Owner GUANGZHOU JET BIOFILTRATION CO LTD
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