Bioreactor for in vitro culture of neurotrophic factors

Abstract

The invention discloses a bioreactor for in vitro culture of neurotrophic factors, which comprises an upper cover and a lower cover which are provided with concave cavities. The concave cavities of the upper cover and the lower cover are closed in opposite directions and fixed to form a box body, a culture plate through which cerebrospinal fluid can pass is arranged in the cavity of the box body,a polyether sulfone semi-permeable membrane is covered on the culture plate, the culture plate is embedded to the middle lower part of the concave cavity of the lower cover and positioned between theupper cover and the lower cover, both the upper part and the lower part of the culture plate are spaces in which the cerebrospinal fluid flows, the upper cover is provided with an input nozzle, the lower cover is provided with an output nozzle, and the upper end face of the upper cover is provided with a plurality of holes through which the air enters the box body. The bioreactor enables the cerebrospinal fluid of a nervous system dysfunction patient to be circulated in the bioreactor and fully mixed with the neurotrophic factors secreted by star-shaped gliocytes to form mixed cerebrospinal fluid containing the neurotrophic factors to be redelivered to the lower cavity of the arachnoid membrane or the ventricular system of the patient, thereby realizing promotion of recovery of functionaldeficiency or functional disorder of the central nervous system caused by diseases or injury, and remarkably improving the life quality of the patient.

Description

technical field [0001] The invention relates to a bioreactor for cell culture, in particular to a bioreactor for in vitro culture of neurotrophic factors used as a device for treating central nervous system dysfunction. Background technique [0002] In the medical field, the neurological dysfunction left after severe craniocerebral injury is a very difficult problem in clinical neuroscience. Many patients are disabled for life as a result, which brings great pain and burden to individuals, families and society. How to improve the quality of life of patients with craniocerebral injury and restore the damaged nerve function has become the focus and difficulty of neuroscience research, and no breakthrough has been made so far. [0003] A large number of clinical studies have shown that in the early stage of craniocerebral injury, if active, effective and reasonable treatment measures are taken, the prognosis of patients with severe craniocerebral injury is expected to be improv...

AI Technical Summary

Problems solved by technology

[0005] In view of the above reasons, how to achieve multi-factor combination, simultaneous administration and continuous uninterrupted administration of neurotrophic factors, and use routes other than intravenous or intramuscular injection to reduce the internal biodegradation, immune rejection Response, blood-brain barrier barrier and other factors can improve the therapeutic effect of drugs, and no effective method has been found yet.

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