175 results about "Dental pulp stem cells" patented technology

The object is to provide a novel use application of a dental pulp stem cell collected from a deciduous tooth or a permanent tooth. Disclosed is a composition for autotransplantation or allotransplantation, which is characterized by comprising a dental pulp stem cell collected from a deciduous tooth or a permanent tooth.

A method for constructing banks of human dental pulp stem cells comprises the steps of acquiring basic information, obtaining dental pulp stem cells, cryopreserving and putting dental pulp stem cells into cell banks and recording and checking information. When the banks of dental pulp stem cells are used, recovery and multiplication culture are carried out in accordance with the actual demands. In the method, the dental pulp stem cells are obtained from dental pulp of waste healthy human teeth and a reserve bank for effective resources is established by using system engineering. A great quantity of dental pulp stem cells with functional activities can be obtained through short-term culture of the human dental pulp stem cells stored in the banks and can be stored for long term without losing the activities. Compared with the prior art, the invention is characterized in that the construction operation specification is simple, easy to grasp, safe and feasible; the bank construction cost is low and the standardization degree is high; and the cells from different age groups adopt different cryopreservation solution so that the activities of the recovered cells are enhanced, thus expanding the sources of the cells and enhancing the activities of the cells. The teeth used in the method are those which are extracted for various reasons and are waste, thus greatly saving the medical resources; therefore, the construction has wide application prospect.

The present invention is directed to therapeutic multifunctional immature dental pulp stem cells (IDPSCs), and IDPSCs multi-lineage compositions. The invention is further directed to the use of IDPSCs and compositions to reduce the risk of and / or treat degenerative diseases or for other medicinal and aesthetic purposes.

The invention relates to the technical field of stem cell culture and in particular relates to a culture medium and application thereof as well as a method for culturing dental pulp stem cells. According to the culture medium, a serum replacement, an EGF (Epidermal Growth Factor), an FGF (Fibroblast Growth Factor), a PDGF (Platelet Derived Growth Factor), a VEGF (Vascular Endothelial Growth Factor), L-glutamine, albumin, insulin and soybean isoflavone are added into a serum-free culture medium; each factor and the soybean isoflavone have a good promotion effect on growth of the dental pulp stem cells. An experiment shows that a cell period can be shortened when the culture medium is used for culturing dental pulp mesenchymal stem cells, the cell proliferation ability is improved, and the purity and dryness of the stem cells are improved; the culture medium does not contain animal derived ingredients including fetal bovine serum and the like, and the stem cells obtained by the culture medium is suitable for clinical application.

The invention relates to a culture medium for human dental pulp stem cells and a preparation method for human dental pulp stem cells and belongs to the technical field of stem cell preparation. The culture medium for human dental pulp stem cells, provided by the invention, is based on an alpha-MEM culture medium and contains 5%-10% of human AB serum by volume percent, 100mu M ascorbic acid, 2mM L-glutamine, 100U / ml penicillin sodium and 100mg / ml streptomycin. The dental pulp stem cells prepared by the culture medium provided by the invention are high in yield, are free from human immunological rejection and can supply reliable seed cells for repairing and regeneration of clinical teeth.

The invention discloses a dental pulp stem cell exosome preparation as well as a preparation method and application thereof, and relates to the technical field of biology. Dental pulp stem cells can express neuronal cell surface markers, can be differentiated to nerve cells and promote the proliferation of the nerve cells, so that the dental pulp stem cells can be used for preparing cell preparations for repairing nerve injuries such as traumatic brain injuries (TBI). The dental pulp stem cells are used for replacing traditional embryonic stem cells, neural stem cells or bone marrow stem cellsto be used for treating the nerve injuries, and have the advantages of being rich in source, simple and convenient to obtain, low in immunogenicity and capable of avoiding ethical problems. Dental pulp stem cell exosomes can accurately target recipient cells and are used for preparing nerve repair preparations for the injuries such as the TBI, and then treatment of the nerve injuries such as theTBI by the exosomes is achieved. The dental pulp stem cell exosomes can also be used for preparing neuroinflammation inhibitors.