41 results about "Dental pulp cell" patented technology

The present disclosed subject matter relates to methods and compositions for restoring a diseased or damaged tooth such that infection is inhibited or eliminated and pulp regeneration is facilitated. The disclosed subject matter also includes a composition comprising a physiologically acceptable matrix seeded with pulp cells. The matrix can be capable of being injected into the pulp chamber of a tooth. In some embodiments, the matrix of a composition includes a hydrogel (e.g., collagen, chitosan, alginate, MATRIGEL™, gelatin, JELL-O®, fibrin), a mesh (e.g., polylactide-coglycolide (PLGA) mesh, polylactide (PLA) mesh, or polyglycolide (PGA) mesh, a cross-linked fiber mesh, a nanofiber mesh, a mesh fabric, biodegradable polymer mesh), a microsphere (biodegradable polymer microsphere, a hydrogel microsphere), or a combination of any of the foregoing. In yet other embodiments, the matrix includes a nanofiber, an artificial three-dimensional scaffold material, or a synthetic three-dimensional scaffold material.

Disclosed is a therapeutic material for cerebral infarction, which can recover a vascular disorder in an area affected by cerebral infarction to improve the brain function. The therapeutic material for cerebral infarction includes a dental pulp stem cell comprising at least one member selected from a CD105-positive cell, an SP cell, a CD24-positive cell, a CD133-positive cell, a CD271-positive cell and a CD150-positive cell. The therapeutic material may additionally include a protein secreted from a dental pulp cell. A transplanted dental pulp stem cell cannot be differentiated directly into a neural progenitor cell or a neurocyte, but is involved indirectly in the promotion of differentiation to eliminate an area affected by cerebral infarction, thereby recovering the affected area into a normal area.

Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

The invention discloses stem cells for specifically expressing a programmed death receptor 1 (PD-1), and particularly relates to the stem cells from an organic cavity. The stem cells comprise but notlimited to dental pulp stem cells, gingival mesenchymal steam cells (GMSCs), periodontal ligament stem cells (PDLSCs), stem cells of apical papilla (SCAPs) and dental follicle stem cells (DFSCs) or any combination thereof, and preferably comprise stem cells from human exfoliated deciduous teeth (SHED) and / or dental pulp mesenchymal stem cells for permanent teeth (DPSC); the stem cells also includemesenchymal stem cells from other tissues, which do not express the PD-1 at first and express the PD-1 after being modified by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), suchas PD-1 plus bone mesenchymal stem cells (BMMSC) modified by the CRISPR. The invention further discloses an identifying and separating method for the stem cells for specifically expressing the PD-1,a method for preparing the PD-1 plus mesenchymal stem cells modified by the CRISPR as well as application of the mesenchymal stem cells for expressing the PD-1, disclosed by the invention, in tissue regeneration, pain relief and treatment of chronic pain and a series of diseases.

Provided is an efficient method of inducing differentiation of a pulp cell into an odontoblast. Also provided is an agent for inducing differentiation capable of inducing differentiation into an odontoblast efficiently. The method of inducing differentiation of a pulp cell into an odontoblast includes using a substance capable of activating a Wnt signaling pathway. Further, the agent for inducing differentiation includes a substance capable of activating a Wnt signaling pathway. Specifically, the substance capable of activating a Wnt signaling pathway is any one selected from sodium perchlorate, sodium perchlorate, lithium chloride, Norrin, and R-Spondin2.

The present invention belongs to the field of organ regenerating technology, and is especially tooth regenerating technology with keratin stem cell and dental pulp stem cell. The technological scheme of the present invention includes: taking out dental pulp tissue form cheek tooth, digesting with digestive enzyme and transferring to culture liquid; culturing for 24 hr, thrice washing with PBS to eliminate un-adherent cell and transferring to culture liquid; further subsequent culturing the adherent cell for 14-16 days; inducing dental pulp stem cell with growth factor MBP4 and FGF10 for 24 hr, recombining in vitro cultured keratin stem cell and dental pulp stem cell to form recombinant ball; culturing the recombinant ball in culture box at 37 deg.c and with CO2 in 5 %, transplanting into oral cavity and culturing for 10-12 weeks to form tooth. The present invention has expanded material source.

The invention relates to a preparation method of engineering dental pulp, which is used for reconstruction of tooth roots. The dental pulp comprises dental pulp cells and an extracellular matrix secreted and synthesized by the dental pulp cells. The dental pulp cells are subjected to amplification culture to form multi-layered dental pulp cell assemblies, and then the dental pulp cell assemblies are arranged layer by layer and molded to form the engineering dental pulp. The dental pulp can form a new dentinal structure in the human body, so that a tooth root can continue growing. The dental pulp is simple in preparation, good in plasticity, moderate in cell density, abundant in extracellular matrix content, free of support material, and short in in-vitro culture time.

The invention provides a pulp capping agent for treating dental pulp lesions as well as a preparation method and application thereof. Bioactive particles release PO4<3-> ions in an oral environment topromote rapid deposition of Ca<2+> and PO4<3-> on the surface of the material, so that a mineralization layer is formed rapidly; the released metal ions and PO4<3-> play a neutralization role and thus the material has the stable pH value in the body fluid, so that the compatibility with the dental pulp cells is improved and the dental pulp cells are promoted to differentiate into the teeth. The dental pulp capping agent can be used in different ways based on different components. The pulp capping agent that is prepared by compounding of bioactive particles such as phosphosilicate glass, phosphosilicate ceramic and a restorative material causing polymerization covers the surfaces of teeth after being subjected to photo-induced polymerization and solidification, so that rapid formation of dental pulp and tooth tissues is induced and thus dental pulp lesions are treated. Moreover, the pulp capping agent prepared by including solid-phase powder and the liquid phase coats the surface of dental pulp uniformly to treat the dental pulp lesions.

The invention provides a method for analyzing different miRNAs (micro-ribonucleic acids) expression profiles of dental pulp stem cells and stem cells from apical papilla by a next-generation sequencing technique. The method includes the steps: preparing single-cell suspensions of the dental pulp stem cells and the stem cells from the apical papilla; separating cells of a logarithmic phase from the single-cell suspensions, and performing marking by the aid of STRO-1-PE; separating and collecting STRO-1 positive cells by the aid of immunomagnetic beads to obtain needed dental pulp stem cells and needed stem cells from the apical papilla; performing extraction by the aid of RNA, and detecting the miRNAs expression profiles of the dental pulp stem cells and the stem cells from the apical papilla by the aid of the next-generation sequencing technique. According to the method, the miRNAs expression profiles of the stem cells are analyzed by the aid of high-throughput next-generation sequencing technique, and the method has profound significance for detecting the different miRNAs expression profiles in the dental pulp stem cells and the stem cells from the apical papilla and knowing characteristics of the stem cells.

The invention relates to a preparation method of an enzymolysis-type bioglass dental pulp repair material, and belongs to the technical field of a medical material. According to the technical scheme of the invention, the dental pulp repair material is prepared by using ethyl orthosilicate, triethyl phosphate and the like as raw materials, so that a body fluid micro environment is alkaline; the enzymolysis-type bioglass dental pulp repair material is beneficial for promoting expression of proteins related to dentinogenesis of dental pulp cells, meanwhile, has excellent bioactivity and ability of forming hydroxyapatite by mineralization and is hopeful to promote formation of reparative dentin; meanwhile, bioglass is dissolved out by ions; regulation of a micro environment of dental pulp tissues is hopeful to promote differentiation of the dental pulp cells to odontoblast, and promotes dentin matrix protein expression; by fixing a protein inhibiting enzyme inside the material, a service life of the dental pulp repair material is effectively prolonged.

The invention discloses an absorbable polylactic acid film for enhancing airtightness of an absorbable root canal post of a deciduous tooth. The absorbable polylactic acid film is prepared by dissolving the following raw materials in parts by weight: 0.5-1.5 parts of polylactic acid, and 5-15 parts of solvent. The absorbable polylactic acid film and the absorbable root canal post are made of the same material, and the absorbable polylactic acid film has no influence on human dental follicle cells, deciduous periodontal ligament cells and deciduous dental pulp cells, cannot result in apoptosis of the human dental follicle cells, is nontoxic, can effectively control the effective strength of the absorbable root canal post, can recover functions and a shape of the deciduous tooth for a long time to the greatest extent, and does not influence normal replacement of deciduous permanent teeth. A preparation method of the film is simple and practicable, and can be used for manufacturing during clinical application. The film is used for residual root and residual crown repair of the deciduous tooth, a crown restoration is good in retention postoperatively, and the film meets the clinical requirement of pediatric dentistry.

It is found that a SVVYGLR peptide (SEQ ID NO: 1) can promote proliferation of mesenchymal cells such as marrow-derived mesenchymal stem cells and dental pulp cells. A carbonate apatite-collagen sponge containing the SVVYGLR peptide (SEQ ID NO: 1) is useful as a biomaterial for the regeneration of bone marrow or dental pulp. It becomes possible to provide a skeletal system biomaterial which is capable of promoting the proliferation of the mesenchymal cells and is useful as artificial bone marrow or artificial dental pulp for repairing a defect in a bone marrow tissue or a dental pulp tissue.

Provided is a method of preparing an embryonic stem cell-like cell, a method of preparing an insulin-secreting cell or pancreatic beta-like cell, a method of preparing a chondrocyte-like cell, a method of preparing a myocyte-like cell, and a method of preparing a hair follicle-like cell. A composition comprising a dental stem cell and an insulin-secreting cell or a pancreatic beta-like cell is also provided. Further, a composition comprising (a) a dental stem cell and (b) a chondrocyte-like cell, a myocyte-like cell, or a hair follicle-like cell is provided. Additionally provided is an insulin-secreting cell or a pancreatic beta-like cell differentiated from a dental stem cell. Further provided is a chondrocyte-like cell, a myocyte-like cell, or a hair follicle-like cell, derived from a dental stem cell.

The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.

It is found that a SVVYGLR peptide (SEQ ID NO: 1) can promote proliferation of mesenchymal cells such as marrow-derived mesenchymal stem cells and dental pulp cells. A carbonate apatite-collagen sponge containing the SVVYGLR peptide (SEQ ID NO: 1) is useful as a biomaterial for the regeneration of bone marrow or dental pulp. It becomes possible to provide a skeletal system biomaterial which is capable of promoting the proliferation of the mesenchymal cells and is useful as artificial bone marrow or artificial dental pulp for repairing a defect in a bone marrow tissue or a dental pulp tissue.

The invention discloses a medicament for repairing dentin, and belongs to the field of dentin regeneration medicaments .The inventor finds that an inhibitor of miR-93 has an effect of promoting dentinregeneration, and the principle is that expression of histone demethylase JMJD3 is improved by inhibiting miR-93, and differentiation of dental pulp cells to odontoblasts and formation of dentin areregulated. The medicament can promote regeneration of natural dentin, can be used as a pulp covering agent used in a pulp covering operation, can overcome the problems that existing pulp covering agents cause diffuse calcification of dental pulp, operation technical requirements are high, tooth discoloration is caused and the like, and has a good application prospect.

The invention provides a human dental pulp cell separation culture method. Glucomannan and sodium alginate are added into a primary culture medium, so that cell adherence growth and replication in a primary culture process can be effectively promoted, and the adherence effect equivalent to that of a traditional coated culture dish or added laminin can be achieved; the pre-coating operation can beomitted, and compared with laminin, the glucomannan and the sodium alginate are more economical, so that the time cost and the economic cost are effectively reduced while the adherence effect is improved, and a large number of high-activity dental pulp stem cells are obtained; meanwhile, the culture medium provided by the invention does not need serum, can effectively promote the growth and replication of the dental pulp cells and improve the differentiation capacity of the cells through the cooperation of various components, and can overcome the defects of the serum on the premise of ensuringthe performance, thereby effectively improving the culture effect of the dental pulp cells.

The present invention relates to a method for producing endothelial progenitor cells, comprising a method for producing induced pluripotent stem cells by using only Oct4 and Sox2 as a reprogramming factor of human dental pulp cells, and differentiating endothelial progenitor cells from the induced pluripotent stem cells produced by the method for producing induced pluripotent stem cells. Moreover, the present invention relates to a method for producing endothelial cells, comprising differentiating endothelial cells from endothelial progenitor cells produced by the method for producing the endothelial progenitor cells. Additionally, the present invention relates to a method for producing smooth muscle cells, comprising differentiating smooth muscle cells from endothelial progenitor cells produced by the method for producing the endothelial progenitor cells.Also, the present invention make a significant contribution to treatment of ischemia disease and heart disease because a patient's dental pulp cell-derived differentiated cells can be used for cell treatment as a result of the cell treatment effect of the endothelial progenitor cells in a mouse model induced to have hind limb ischemia or myocardial infarction.

Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

The invention relates to a preparation method and application of a dental pulp and dentin complex. The preparation method of the dental pulp and dentin complex comprises the following steps of: primary culture and subculture of dental pulp cells; preparing a liquid-phase concentrated growth factor; and mixing the cultured dental pulp cells with the liquid-phase concentrated growth factor to obtain a mixed material. Therefore, the dental pulp and dentin complex structure is prepared, and regeneration of dental pulp tissue is effectively promoted. According to the preparation method and application of the dental pulp and dentin complex of the invention, the autologous dental pulp cells and liquid-phase concentrated growth factor are mixed to be used as a young permanent tooth pulp tissue engineering scaffold, so that continuous growth of tooth roots can be promoted, and pains of patients can be relieved; the non-immune material is taken, the ectopic dental pulp-like tissue can be generated by transplantation of the dental pulp stem cells, the quantity of transplanted cells can be controlled, cell subgroups with the best potential effect on regeneration of the dental pulp and dentin complex can be screened, dental pulp regeneration is promoted, and dental pulp activity is recovered; and the liquid-phase growth factor can accelerate the wound healing process, and tissue regeneration is facilitated by stimulating cell proliferation and differentiation and promoting stem cell migration.

The invention discloses a bioactive glass / bionic functional polypeptide complex as well as a preparation method and application thereof, belongs to the technical field of biology, and solves the technical problems that in the prior art, a bionic mineralization functional polypeptide gel preparation lacks a calcium and phosphorus source necessary for remineralization, and cannot safely retain the vitality of a dental pulp and dentin complex and induce dentin regeneration. The bioactive glass / bionic functional polypeptide complex takes mesoporous bioactive glass as a carrier to load a bionic functional polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The preparation method comprises the following steps: adding the mesoporous bioactive glass into a buffer solution, dispersing, adding the bionic functional polypeptide, uniformly stirring, carrying out solid-liquid separation, and cleaning and drying the obtained solid, thereby obtaining the bioactive glass / bionic functional polypeptide complex. The bioactive glass / bionic functional polypeptide complex disclosed by the invention not only has a bionic mineralization effect on hard tissues, but also can provide a calcium and phosphorus ion source required by mineralization, further has a mineralization promoting effect on dental pulp cells, and can further promote dentin repair.

The present invention provides a serum-free endothelial cell differentiation culture medium comprising (a) a basal culture medium and (b) an endothelial cell differentiation combination of EGF, FGF and VEGF protein, wherein the amount of EGF is higher than the amount of FGF protein. The present invention further provides a process for the preparation of cellular aggregate suspensions comprising differentiated endothelial cells from dental stem cells using the serum-free medium, as well as the use of the resulting suspension in therapy.

The invention relates to the technical field of dental reagents and particularly relates to the technical field of in-vitro evaluation of a pulp capping material, and discloses a preparation method ofan in-vitro evaluation model of the pulp capping material. The method is characterized by including steps of: S1) obtaining an in-vitro tooth; S2) separating dental pulp cells; S3) amplifying the cells; S4) adding ECM to perform ball-forming growth; S5) performing continuous induction to prepare a human dentin extract, and adding a culture medium containing the dentin extract into a culture plateto performing continuous induction of cells, thus preparing the human dental pulp organ model. In the method, the human in-vitro dental pulp cells are selected, then cell separation and amplificationand a creative cell ball-forming growth method are carried out, finally continuous induction is carried out. The method is high in cell growth efficiency, and highly simulates the dental pulp organ of human, thereby increasing in-vitro evaluation efficiency and accuracy of the whole material.

The invention discloses a medicine for restoring dentin and belongs to the field of medicine for dentin regeneration. The inventors found through research that the inhibitor of miR-93 has the effect of promoting dentin regeneration. The principle is to increase the expression of histone demethylase JMJD3 by inhibiting miR-93, and regulate the differentiation of dental pulp cells into odontoblasts. and dentin formation. The invention can promote natural dentin regeneration, can be used as a pulp capping agent used in pulp capping, can overcome the problems of diffuse calcification of dental pulp caused by existing pulp capping agents, high technical requirements for operation, and cause tooth discoloration, etc. application prospects.

The embodiment of the invention discloses a culturing cryopreservation system for amplifying dental pulp stem cells in vitro. The culturing cryopreservation system comprises a dental pulp stem cellextracting reagent, a dental pulp stem cell culturing reagent and a dental pulp stem cell preservation reagent, and the culturing reagent comprises dental pulp stem cell original generation culturing liquid, dental pulp stem cell passage culturing agent anddental pulp stem cell digestive liquid. The embodiment of the invention further provides a culturing cryopreservation kit and method for amplifying the dental pulp stem cells in vitro. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro comprises all processes from preservation and conveying of the dental pulp stem cells in teeth to cryopreservation for the dental pulp stem cells. Storage of the dental pulp stem cells is simple in operation, and a large number of high-purity dental pulp cells can be obtained. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro reduces resource waste caused by preparation many times for different reagents and reduces the risk of cross contamination with other samples.

The invention discloses an application of surface-modified titanium and titanium alloy for dental pulp coronal sealing. The surface modification is used to improve the induction performance of titanium and titanium alloy on adhesion and differentiation of dental pulp cells; and the surface-modified titanium and titanium alloy are used for covering dental pulp tissue after vital pulpotomy or dentalpulp regeneration, so that dental pulp forms a dentin integrated material, wherein the surface-modified titanium and titanium alloy can be selected from micro-arc oxidation composite graphene oxide coating-modified titanium and titanium alloy. The surface-modified titanium or titanium alloy is used as a dental pulp coronal sealing material to promote the adhesion, proliferation, and dentinogenesis differentiation of the dental pulp stem cells to form the physiological dentin structure on the top of the exposed dental pulp.

This patent application relates to the technical field of dental preparations, in particular to the technical field of in vitro evaluation of pulp capping materials. The invention discloses a method for preparing an in vitro evaluation model of a pulp capping material, which is characterized in that it comprises the following steps: S1. Obtaining an isolated tooth; S2. Isolating dental pulp cells; S3. Expanding cells; S4. Adding ECM to form spheres and grow ; S5. Continuous induction, preparing human dentine extract, adding a medium containing dentine extract to a culture plate to continuously induce cells, and making a human dental pulp organoid model. The present invention selects the isolated human dental pulp cells, undergoes cell separation and expansion, adopts a invasive cell growth method, and finally adopts a continuous induction method, the growth efficiency of the cells is high, and the human dental pulp organs are highly simulated. The efficiency and accuracy of the in vitro evaluation of the entire material are improved.

The invention discloses novel tooth whitening gel and a preparation method thereof. The novel tooth whitening gel comprises an oxidizing agent, an activating agent, a thickening agent and water. The preparation method of the novel tooth whitening gel comprises the following steps that after the corresponding activating agent is fully reacted with the oxidizing agent according to a certain mass ratio, the thickening agent is added and mixing is carried out, uniform stirring is carried out, and standing is carried out to obtain the novel tooth whitening gel. Phosphate is used for activating persulfate to obtain the whitening gel containing oxygen free radicals, so that the degradation of pigments in tooth hard tissues is realized, the whitening effect is achieved, and meanwhile, the demineralization of the enamel surface and the adverse stimulation to dental pulp cells can be effectively reduced.