The invention discloses a bioactive glass / bionic functional polypeptide complex as well as a preparation method and application thereof, belongs to the technical field of biology, and solves the technical problems that in the prior art, a bionic mineralization functional polypeptide gel preparation lacks a calcium and phosphorus source necessary for remineralization, and cannot safely retain the vitality of a dental pulp and dentin complex and induce dentin regeneration. The bioactive glass / bionic functional polypeptide complex takes mesoporous bioactive glass as a carrier to load a bionic functional polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The preparation method comprises the following steps: adding the mesoporous bioactive glass into a buffer solution, dispersing, adding the bionic functional polypeptide, uniformly stirring, carrying out solid-liquid separation, and cleaning and drying the obtained solid, thereby obtaining the bioactive glass / bionic functional polypeptide complex. The bioactive glass / bionic functional polypeptide complex disclosed by the invention not only has a bionic mineralization effect on hard tissues, but also can provide a calcium and phosphorus ion source required by mineralization, further has a mineralization promoting effect on dental pulp cells, and can further promote dentin repair.