Culturing cryopreservation system, method and kit for amplifying dental pulp stem cells in vitro

Abstract

The embodiment of the invention discloses a culturing cryopreservation system for amplifying dental pulp stem cells in vitro. The culturing cryopreservation system comprises a dental pulp stem cellextracting reagent, a dental pulp stem cell culturing reagent and a dental pulp stem cell preservation reagent, and the culturing reagent comprises dental pulp stem cell original generation culturing liquid, dental pulp stem cell passage culturing agent anddental pulp stem cell digestive liquid. The embodiment of the invention further provides a culturing cryopreservation kit and method for amplifying the dental pulp stem cells in vitro. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro comprises all processes from preservation and conveying of the dental pulp stem cells in teeth to cryopreservation for the dental pulp stem cells. Storage of the dental pulp stem cells is simple in operation, and a large number of high-purity dental pulp cells can be obtained. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro reduces resource waste caused by preparation many times for different reagents and reduces the risk of cross contamination with other samples.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of stem cell culture, and in particular to a culture and cryopreservation system, method and kit for in vitro expansion of dental pulp stem cells. Background technique [0002] Periodontitis is a chronic infectious disease involving four types of periodontal supporting tissues, often leading to inflammatory destruction of periodontal supporting tissues. Common causes are bacterial plaque, calculus, traumatic occlusion, food impaction, bad restorations, and mouth breathing. Periodontitis has four major characteristics, namely the formation of periodontal pockets, inflammation of the pocket wall, alveolar bone resorption, and gradual loosening of teeth. Severe periodontitis can cause tooth loss, leading to poor masticatory function and indigestion and gastrointestinal diseases, affecting the health of the whole body and mind. The main purpose of treating periodontitis is to eliminat...

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Problems solved by technology

[0005] For this reason, the embodiment of the present invention provides a culture and cryopreservation system, method and kit for in vitro expansion of dental pulp stem cells to solve the problem of reagent waste and crossover caused by the small amount of samples and different sources of samples in the prior art. pollution problem

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