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Culturing cryopreservation system, method and kit for amplifying dental pulp stem cells in vitro

A technique for in vitro expansion of dental pulp stem cells, which is applied in the field of culture and cryopreservation system for in vitro expansion of dental pulp stem cells, can solve the problems of reagent waste, small sample size, and different sample sources, so as to reduce resource waste and cross-contamination risk, the effect of easy storage and operation

Inactive Publication Date: 2019-04-16
北京博奥晶典启衡生物科技有限公司
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Problems solved by technology

[0005] For this reason, the embodiment of the present invention provides a culture and cryopreservation system, method and kit for in vitro expansion of dental pulp stem cells to solve the problem of reagent waste and crossover caused by the small amount of samples and different sources of samples in the prior art. pollution problem

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  • Culturing cryopreservation system, method and kit for amplifying dental pulp stem cells in vitro
  • Culturing cryopreservation system, method and kit for amplifying dental pulp stem cells in vitro
  • Culturing cryopreservation system, method and kit for amplifying dental pulp stem cells in vitro

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[0033] The implementation mode of the present invention is illustrated by specific specific examples below, and those who are familiar with this technology can easily understand other advantages and effects of the present invention from the contents disclosed in this description. Obviously, the described embodiments are a part of the present invention. , but not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0034]An embodiment of the present invention provides a culture and cryopreservation system for expanding dental pulp stem cells in vitro. The culture and cryopreservation system includes dental pulp stem cell extraction reagents, dental pulp stem cell culture reagents, and dental pulp stem cell preservation reagents, wherein the culture reagents include dental pulp Stem cell primary culture medi...

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Abstract

The embodiment of the invention discloses a culturing cryopreservation system for amplifying dental pulp stem cells in vitro. The culturing cryopreservation system comprises a dental pulp stem cellextracting reagent, a dental pulp stem cell culturing reagent and a dental pulp stem cell preservation reagent, and the culturing reagent comprises dental pulp stem cell original generation culturing liquid, dental pulp stem cell passage culturing agent anddental pulp stem cell digestive liquid. The embodiment of the invention further provides a culturing cryopreservation kit and method for amplifying the dental pulp stem cells in vitro. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro comprises all processes from preservation and conveying of the dental pulp stem cells in teeth to cryopreservation for the dental pulp stem cells. Storage of the dental pulp stem cells is simple in operation, and a large number of high-purity dental pulp cells can be obtained. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro reduces resource waste caused by preparation many times for different reagents and reduces the risk of cross contamination with other samples.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of stem cell culture, and in particular to a culture and cryopreservation system, method and kit for in vitro expansion of dental pulp stem cells. Background technique [0002] Periodontitis is a chronic infectious disease involving four types of periodontal supporting tissues, often leading to inflammatory destruction of periodontal supporting tissues. Common causes are bacterial plaque, calculus, traumatic occlusion, food impaction, bad restorations, and mouth breathing. Periodontitis has four major characteristics, namely the formation of periodontal pockets, inflammation of the pocket wall, alveolar bone resorption, and gradual loosening of teeth. Severe periodontitis can cause tooth loss, leading to poor masticatory function and indigestion and gastrointestinal diseases, affecting the health of the whole body and mind. The main purpose of treating periodontitis is to eliminat...

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Application Information

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IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226C12N5/0664C12N2500/32C12N2500/38C12N2509/00
Inventor 沈力坚李明辉王健徐云龙侯志勇周书行弓培翟珊莉
Owner 北京博奥晶典启衡生物科技有限公司
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