Preparation method of in-vitro evaluation model of pulp capping material

Abstract

The invention relates to the technical field of dental reagents and particularly relates to the technical field of in-vitro evaluation of a pulp capping material, and discloses a preparation method ofan in-vitro evaluation model of the pulp capping material. The method is characterized by including steps of: S1) obtaining an in-vitro tooth; S2) separating dental pulp cells; S3) amplifying the cells; S4) adding ECM to perform ball-forming growth; S5) performing continuous induction to prepare a human dentin extract, and adding a culture medium containing the dentin extract into a culture plateto performing continuous induction of cells, thus preparing the human dental pulp organ model. In the method, the human in-vitro dental pulp cells are selected, then cell separation and amplificationand a creative cell ball-forming growth method are carried out, finally continuous induction is carried out. The method is high in cell growth efficiency, and highly simulates the dental pulp organ of human, thereby increasing in-vitro evaluation efficiency and accuracy of the whole material.

Description

technical field [0001] The invention belongs to the technical field of dental preparations, in particular to the technical field of in vitro evaluation of pulp capping materials. Background technique [0002] At present, every year in our country, a large number of patients have dental pulp exposed or nearly exposed due to caries or trauma. Infection can form lesions, causing or adding arthritis, pericarditis, and various eye diseases and other systemic diseases. At present, in view of the above situation, in the absence of obvious irreversible infection, the affected tooth needs to receive pulp capping treatment, that is, the pulp capping material is directly placed on the exposed pulp tissue to seal the exposed pulp, avoid infection and further stimulation, and promote The role of local dentin bridge formation. Pulp capping, also known as pulp capping, is a method of protecting the living pulp, that is, covering the dentin near the pulp or the exposed pulp wound with a p...

AI Technical Summary

Problems solved by technology

The current in vitro cell model is to inoculate human dental pulp cells on a culture plate, and the cells grow in a single layer, while the cells grow in a three-dimensional state in vivo. Using this model for drug screening and detection cannot reflect the actual situation in the human body

There are many options for animal in vivo models, such as rodents, canines, primates, etc., using rodents or canines, the dental pulp cells and human dental pulp tissue cells are very different, such as mouse Anterior teeth can grow all their lives, so this method can only reflect the toxicity or biocompatibility of drugs on dental pulp tissue to a certain extent, but there are differences from human dental pulp tissue; while using primates, it is costly Huge, and there is a possibility of violating the requirements of ethical review

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