Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of engineering dental pulp

An engineering and endodontic technology, applied in biochemical equipment and methods, culture process, bone/connective tissue cells, etc., can solve problems such as lack of natural root morphological structure, avoid poor compatibility and easy preparation , the effect of appropriate cell density

Active Publication Date: 2017-06-27
XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] American scholar Huang GT et al. combined fibrin glue with root-end dental papilla stem cells to form cell clusters, implanted them in the root cap and subcutaneously on the back of immunodeficient mice, and took out the implants 3 to 4 months later. A regenerated dentin layer, odontoblast layer, and vascularized pulp tissue were observed, but lacked the morphology of a native tooth root
There is no report on the establishment of engineered dental pulp for root reconstruction and regeneration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of engineering dental pulp
  • Preparation method of engineering dental pulp
  • Preparation method of engineering dental pulp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Embodiment 1, one of the preparation methods of engineered dental pulp

[0034] Its preparation steps are as follows:

[0035] Step 1. Dental pulp cell culture: Mince the pulp tissue of deciduous teeth of miniature pigs, digest with 0.2g / L type Ⅰ collagenase and 0.1g / L Dispase enzyme for 1 hour until the tissue is loose, blow and beat the pulp with a straw The organization is fully dispersed. Sieve through a sieve to form a single cell suspension, discard the supernatant after centrifugation, then add dental pulp cell culture medium to resuspend the cells, transfer to a cell culture bottle, 37 ° C, 5% CO 2 Cultured in an incubator, the medium was changed every other day, and the cells were cultured to 90% confluence and then passaged to obtain the second generation of dental pulp cells.

[0036] The composition of the dental pulp cell culture medium A is: 10% (v / v) fetal bovine serum, 0.2 mg / mL glutamine, 75 ng / mL basic fibroblast growth factor in commercial α-MEM cul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of engineering dental pulp, which is used for reconstruction of tooth roots. The dental pulp comprises dental pulp cells and an extracellular matrix secreted and synthesized by the dental pulp cells. The dental pulp cells are subjected to amplification culture to form multi-layered dental pulp cell assemblies, and then the dental pulp cell assemblies are arranged layer by layer and molded to form the engineering dental pulp. The dental pulp can form a new dentinal structure in the human body, so that a tooth root can continue growing. The dental pulp is simple in preparation, good in plasticity, moderate in cell density, abundant in extracellular matrix content, free of support material, and short in in-vitro culture time.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a preparation method of engineered dental pulp for tooth root reconstruction and regeneration. Background technique [0002] After the tooth erupts, the tooth root relies on the living pulp cells in the root canal and periapical area to continue to develop and form a tooth root. It takes about 3 to 5 years for the tooth root to complete its development. When the root develops due to trauma, caries, or deformed central cusp, etc., the pulp infection and cell necrosis can stop the development of the root. For young permanent teeth with undeveloped roots, the length and thickness of the roots are insufficient, and the apical foramen is open. Such teeth cannot withstand normal chewing force, and the service life is shortened. Therefore, the young permanent teeth continue to develop and the apical foramen closes. , the recovery of the periapical tissue has impo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38C12N5/0775
CPCA61L27/3804A61L27/3865A61L2430/12C12N5/0664C12N2500/32C12N2500/38C12N2501/115C12N2501/15C12N2501/39C12N2501/999
Inventor 张勇杰
Owner XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com