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Low-serum protein-free culture medium applicable to Marc-145 cell growth and preparation method thereof

A protein-free medium and low serum technology, applied in the field of low-serum protein-free medium and its preparation, can solve the problems of reducing yield, increasing the difficulty of separation and purification of downstream products, reducing the amount of serum used, etc. The effect of improving stability and improving efficiency

Inactive Publication Date: 2015-12-23
南京三生生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, animal-derived components such as bovine serum albumin, transferrin, insulin, recombinant epidermal growth factor, and plant-derived protein components such as soybean hydrolyzed protein are often added to reduce the amount of serum used.
Then these added proteins often have a great impact on biological products (such as vaccines), increasing the difficulty of separation and purification of downstream products, thereby reducing the yield

Method used

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  • Low-serum protein-free culture medium applicable to Marc-145 cell growth and preparation method thereof
  • Low-serum protein-free culture medium applicable to Marc-145 cell growth and preparation method thereof
  • Low-serum protein-free culture medium applicable to Marc-145 cell growth and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example is used to prepare a low-serum, protein-free cell culture medium suitable for the growth of Marc-145 cells.

[0045] 1. The formulation of the culture medium

[0046] Table 1 medium formula

[0047]

[0048]

[0049]

[0050] 2. Preparation method

[0051] (1) Get the dried components in the formula and put them into the ball mill tank respectively, and ball mill for 2 hours to get the culture medium dry powder;

[0052] (2) A total of 16.53 g of the dry medium powder prepared in step (1) was dissolved in 900 ml of ultrapure water, then the volume was adjusted to 1 L with ultrapure water, and the bacterium filter membrane with a pore size of 0.22 μm was used to filter and sterilize, to obtain Low-serum protein-free liquid medium suitable for the growth of Marc-145 cells, refrigerated at 6°C for use.

Embodiment 2

[0054] This example is used to prepare a low-serum, protein-free cell culture medium suitable for the growth of Marc-145 cells. 1. The formulation of the culture medium

[0055] Table 2 medium formula

[0056]

[0057]

[0058]

[0059] 2. Preparation method

[0060] (1) Get the dried components in the formula and put them into the ball mill tank respectively, and ball mill for 1.5 hours to get the culture medium dry powder;

[0061] (2) Dissolve the dry medium powder prepared in step (1) in 900ml ultrapure water, then dilute it to 1L with ultrapure water, and filter and sterilize it with a 0.22 μm filter membrane to obtain a solution suitable for Marc Low-serum protein-free liquid medium for -145 cell growth, refrigerated at 8°C for later use.

Embodiment 3

[0063] This example is used to prepare a low-serum, protein-free cell culture medium suitable for the growth of Marc-145 cells.

[0064] 1. The formulation of the culture medium

[0065] Table 3 medium formula

[0066]

[0067]

[0068]

[0069]

[0070] 2. Preparation method

[0071] (1) Get the dried components in the formula and put them into the ball mill tank respectively, and ball mill for 3 hours to get the culture medium dry powder;

[0072] (2) Dissolve the dry medium powder prepared in step (1) in 900ml ultrapure water, then dilute it to 1L with ultrapure water, and filter and sterilize it with a 0.22 μm filter membrane to obtain a solution suitable for Marc Low-serum protein-free liquid medium for -145 cell growth, refrigerated at 4°C for later use.

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Abstract

The invention belongs to the field of cell culture and particularly relates to a low-serum protein-free culture medium applicable to Marc-145 cell growth and a preparation method thereof. The culture medium comprises amino acid, vitamin, inorganic salt, micro elements and other ingredients. The low-serum protein-free culture medium applicable to Marc-145 cell growth and the preparation method of the low-serum protein-free culture medium have the advantages of being free of protein and animal source ingredients and definite in chemical ingredient, the interference to cell culture of serum is industrially reduced, the production cost is lowered, and great convenience is brought to industrial production of cells.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to a low-serum protein-free medium suitable for Marc-145 cell growth and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcinereproductiveandrespiratorysyndrome, PRRS), commonly known as porcine blue ear disease, has been popular since the late 1980s. It was officially named by the International Veterinary Bureau in 1992. It is caused by porcine reproductive and respiratory syndrome virus. A contagious disease characterized by reproductive failure and respiratory symptoms in piglets. It mainly affects pigs, especially sows. The disease seriously affects their reproductive function. The main clinical features are miscarriage, stillbirth, mummified fetus, weak fetus, and dyspnea. It has spread rapidly throughout the pig-raising countries in the world, and it is more likely to become popular in areas with dense pig po...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor 陈文均徐念沁陈巧云
Owner 南京三生生物技术股份有限公司
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