306results about How to "Inhibition of proliferative ability" patented technology

The invention provides a total sesquiterpene lactone extract of centipeda minima, a preparation method and application thereof. The preparation method comprises the following steps: crushing dried total centipeda minima, and extracting centipeda minima volatile oil by a supercritical carbon dioxide extracting method; and enriching the centipeda minima volatile oil through macroporous adsorption resin column chromatography or silica gel column chromatography to obtain the total sesquiterpene lactone extract of the centipeda minima. According to the method, the total sesquiterpene lactone with effect of inhibiting tumor proliferation is extracted from Chinese medicament centipeda minima and is enriched for the first time; the extract is rich in short-leaf geraniin; the extract contains at least nine sesquiterpene lactone compounds, wherein the angelic acid centipeda minima and the isobutyric acid centipeda minima are new active sesquiterpene lactone compounds; by the preparation method, the yield is high; the method is suitable for industrialized production; and the extract has the effect of inhibiting tumor cell proliferation, and can be used for preparing various medicaments or health-care products with anti-tumor effect.

The invention relates to a monoclonal antibody of AP‑2alpha and its use in the preparation of medicines for treating cervical cancer. The monoclonal antibody has a good effect of inhibiting the proliferation of cervical cancer cells and has broad application prospects.

The invention relates to the field of medical chemistry and pharmacotherapeutics, and particularly relates to an NO donor type matrine derivative and a preparation method thereof and application in pharmacy. The compounds have an anti-tumor effect and can be used for preparing anti-tumor medicines. The invention also relates to a preparation method of the compounds.

The invention discloses an environment-friendly textile printing pearlescent adhesive cement and a preparation method and application thereof. The adhesive cement comprises the following components in percentage by weight: 49 to 39 percent of environment-friendly ultra-soft adhesive, 15 to 25 percent of rutile silvery white pearlescent powder in the particle size range of between 5 and 25 microns, 15 to 25 percent of anatase silvery white pearlescent powder in the particle size range of between 10 and 60 microns, 4 to 6 percent of glycol, 1 to 2 percent of dispersing agent, 1 to 3 percent of leveling agent, 1 to 2 percent of mould-proof antibacterial auxiliary agent, 2 to 4 percent of nano negative ion far-infrared powder and 2 to 4 percent of thickening agent. The adhesive cement cannot block a screen during screen printing, has high weather resistance and has the obvious advantages of environment friendliness, no toxic hazard, high-temperature resistance, acid-base resistance, incapability of spontaneous combustion and combustion supporting, high oxidation resistance, high covering power and the like which are obviously distinguished from those of the common dye and pigment.

The invention relates to a traditional medicine with vessel growth resistance function, wherein it is formed by matrine and kukoline at 1:0.05-1 ratio. It can improve treatment effect and reduce toxicity, on atrophic arthritis, cancer, etc.

The present invention relates to an AP-2alpha monoclonal antibody and an application to preparation of drugs for treating cervical cancers. The monoclonal antibody has the relatively good cervical cancer cell proliferation inhibiting effect, thereby having the wide application prospect.

The invention relates to a recombinant adeno-associated virus. The recombinant adeno-associated virus is formed by inserting a tumor antigen gene into a shuttle expression vector pAAV-MCS. The invention also provides a construction method and an application of the recombinant adeno-associated virus. According to the invention, the recombinant adeno-associated virus carrying a tumor-associated antigen gene is utilized for infecting DC cells and expresses tumor-associated antigen protein in the DC cells, and a PD-1 gene is also combined for silencing CTL cells in the application method of the recombinant adeno-associated virus.

The invention relates to the field of biotechnology, in particular to a fully human anti-VEGF (Vascular Endothelial Growth Factor) monoclonal antibody and a preparation method as well as application thereof. In the invention, a large-capacity natural human phage antibody library is constructed; a fully human anti-VEGF antibody 11A7 is screened from the library; an amino acid sequence of a heavy chain variable region of the antibody is shown as SEQ ID NO: 6; and an amino acid sequence of a light chain variable region of the antibody is shown as SEQ ID NO: 8. The invention also discloses the preparation method of the 11A7 antibody, a nucleotide sequence encoding the 11A7 antibody, a nucleic acid sequence-containing expression vector and a host cell. Compared with a humanized antibody bevacizumab, the 11A7 antibody has higher affinity of the antibody and higher capacity of suppressing proliferation of tumor cells and can be used for obviously suppressing growth of tumors and preparing anti-tumor medicaments.

The invention relates to polyphenol substances, a synthesis method and use thereof, in particular to flavanone derivatives, a preparation method and use thereof, which solves the problem that the prior natural flavanone compounds are difficult to extract, not easy to obtain and incapable of extensive use. The compounds have a structure (as above), wherein R1, R2 and R3 are selected from hydrogen, 1 to 8 carbonic alkyl groups, halogen, nitro group, 1 to 8 carbonic alkoxy or amino groups respectively; R4 and R5 can be selected from 1 to 8 carbonic alkyl groups, 1 to 8 alkoxy or alkenyl groups respectively; and the compounds are obtained through the reaction of compound 6-hydroxy-2,4-hydroxy acetophenone substances and aromatic aldehydes or heterocyclic aldehyde substances in polyalcohol solvent by taking high-boiling-point acid as a catalyst, and can be applied to the preparation of medicaments for resisting tumors and cardiovascular and cerebrovascular diseases. The flavanone derivatives have the activity of resisting tumors and inhibiting the cardiovascular and cerebrovascular diseases, thereby opening up a new way for preparing the medicaments and bringing new breakthroughs for the medicament field. Moreover, the synthesis method has the advantages of mild conditions and easy operation.

The invention discloses the use of human ZFX gene and related medicines. The invention discloses the application of human ZFX gene in tumor treatment, tumor diagnosis and medicine preparation. The present invention further constructs human ZFX gene small molecule interference RNA, human ZFX gene interference nucleic acid construct, human ZFX gene interference lentivirus and discloses their applications. The siRNA provided by the present invention or the nucleic acid construct containing the siRNA sequence, and the lentivirus can specifically inhibit the expression of the human ZFX gene, especially the lentivirus, which can efficiently infect target cells and efficiently inhibit the expression of the ZFX gene in the target cells , and then inhibit the growth of tumor cells and promote the apoptosis of tumor cells, which is of great significance in tumor therapy.

The invention discloses a 5-hydroxy-2'-nitroaurone or 5-hydroxy-4'-nitroaurone derivatives and application thereof, wherein the derivatives are a compound represented as the formula (I), an isomer or a pharmaceutically acceptable salt thereof. The compound is excellent in inhibition activity on endothelial cells and tumor cells comprising A549, Bel-74021 and MCF-7, and is excellent in inhibition effects on migration and invasion of the tumor cell A549.

The invention relates to application of bone marrow mesenchymal stem cells combined with monoclonal antibodies in cancer treatment. Monoclonal antibodies specifically aiming at the liver cancer are obtained through screening, the antibodies have a good effect of inhibiting proliferation of liver cancer cells Huh-7 and HepG2 at the same time, meanwhile it is confirmed in a mouse model test that the monoclonal antibodies have a good effect of inhibiting proliferation of tumors in a mouse body, and the antibodies and mesenchymal stem cells are combined for treating liver cancer to generate a synergistic effect, so that the antibodies have a relatively good application prospect.

The invention belongs to the technical field of biomedicine, in particular to the technical field of an albumin medicament. In order to solve the technical problems of short half-life period in vivo and high use level of the existing ATWLPPR peptide, the invention provides an albumin variant. The albumin variant is obtained by mutating a KEQLK peptide section at the tail end of albumin C to a WLPPR peptide section. The albumin variant completely reserves the functions of human albumin and simultaneously has a new ATWLPPR sequence function. Experiments in vitro prove that the albumin variant can obviously inhibit the proliferation and migration of vascular endothelial cells induced by VEGF (Vascular Endothelial Growth Factor) in vitro, and the efficiency of the albumin variant corresponds to that of small peptide. Moreover, when the albumin variant is injected into animals, the efficiency of inhibiting the cornea angiogenesis functions of rabbits is 40-60 times higher than that of the small peptide with the same concentration. The albumin variant can be used as a novel anti-angiogenesis medicament with high efficiency.

The invention discloses a method for screening protein biological markers related to hepatic stellate cell activation and hepatic fibrosis. The method comprises the preparation of the automatic aviation models of hepatic stellate cells subjected to isolation culture and in vitro culture and the preparation of static and activated hepatic stellate cell protein samples. The invention has the advantages that the method is simple, and the operation is easy.

The present invention relates to a simulated lactic acid bacterium flavored vegetarian sausage. The vegetarian sausage mainly comprises the following components: textured soybean proteins, water, soybean oil and lactic acid bacteria. The simulated lactic acid bacterium flavored vegetarian sausage is prepared by vegetarian meat stirring, compound ingredient stirring, lactic acid bacterium inoculating, fermenting, sausage filling, shaping and cooking, cooling and sausage casing stripping, vacuum packaging, and sterilizing. The proteins of soybeans in the prepared simulated lactic acid bacterium flavored vegetarian sausage contains isoflavones, the lactic acid bacteria promote absorptions of nutrients of proteins, monosaccharide, calcium, magnesium, etc., and the vegetarian sausage produces a large amount of beneficial substances of vitamin B groups, etc., inhibits cholesterol absorption, lowers blood lipids and blood pressure, has good chewiness, is rich in flavor and full and life-like in appearance, and has shape characteristics of meat products of the sausages.