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Use of ganoderic acid T and ganoderic and Me in tumour growth or proliferation inhibitor

A technology of Ganoderma lucidum acid and inhibitor, which is applied in the application field of Ganoderma lucidic acid T and Ganoderma lucidum acid Me in tumor growth or proliferation inhibitors, can solve the problems such as unreported Ganoderma lucidic acid monomers, etc. The effect of less toxicity, reducing the possibility of adverse effects

Inactive Publication Date: 2005-12-21
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the ganod

Method used

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  • Use of ganoderic acid T and ganoderic and Me in tumour growth or proliferation inhibitor
  • Use of ganoderic acid T and ganoderic and Me in tumour growth or proliferation inhibitor
  • Use of ganoderic acid T and ganoderic and Me in tumour growth or proliferation inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] Toxic effects of GA-T and GA-Me on HeLa cells:

[0031] Both GA-T and GA-Me are pure natural products isolated from Ganoderma lucidum mycelium produced by fermentation, with a purity greater than 99%, and diluted with DMEM culture solution to the required concentration. The thiazole blue (MTT) rapid colorimetric method was used to determine the toxic effects of GA-T and GA-Me on HeLa cells. The logarithmic growth phase cells (10 6 cell·ml -1 ) Inoculate a 96-well culture plate, 0.2ml per well, respectively add a certain concentration of GA-T and GA-Me treatment, each concentration in parallel 4 wells, the control group add an equal volume of culture medium, set 37 ℃, 5% CO 2 And incubate in an incubator with saturated humidity for 1-4 days, add 5mg·ml to each well 4 hours before the end of the experiment -1MTT 10μl, add 0.04N dimethyl sulfoxide (DMSO) to each well after incubation, 150μl per well, shake for 10min, wait unt...

Example Embodiment

[0033] Example 2

[0034] Toxic effects of GA-T and GA-Me on 95-D cells:

[0035] Both GA-T and GA-Me are pure natural products isolated from Ganoderma lucidum mycelium produced by fermentation, with a purity greater than 99%, and diluted with RPMI-1640 culture solution to the required concentration. The rapid colorimetric method of thiazole blue (MTT) was used to determine the toxicity of GA-T and GA-Me on 95-D cells. The logarithmic growth phase cells (10 6 cell·ml -1 ) Inoculate a 96-well culture plate, 0.2ml per well, respectively add a certain concentration of GA-T and GA-Me treatment, each concentration in parallel 4 wells, the control group add the same volume of culture medium, set 37 ℃, 5% CO 2 Incubate in an incubator with saturated humidity for 1-4 days, add 5mg·ml to each well 4 hours before the end of the experiment -1 MTT 10μl, add 0.04N DMSO to each well after the incubation, 150μl per well, shake for 10min, wait until the MTT reduction ...

Example Embodiment

[0037] Example 3

[0038] Comparison of the cytotoxicity of GA-T and GA-Me on several tumor cells and normal cell L02:

[0039] Both GA-T and GA-Me are pure natural products isolated from Ganoderma lucidum mycelium produced by fermentation, with a purity greater than 99%, and diluted with DMEM or RPMI-1640 culture solution to the required concentration. The rapid colorimetric method of thiazole blue (MTT) was used to determine the toxicity of GA-T and GA-Me on several cells. The logarithmic growth phase cells (10 6 cell·ml -1 ) Inoculate a 96-well culture plate, 0.2ml per well, respectively add a certain concentration of GA-T and GA-Me treatment, each concentration in parallel 4 wells, the control group add the same volume of culture medium, set 37 ℃, 5% CO 2 Incubate in an incubator with saturated humidity for 1-4 days, add 5mg·ml to each well 4 hours before the end of the experiment -1 MTT 10μl, after incubation, add 0.04N DMSO (dimethyl sulfoxide) to ea...

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Abstract

The present invention discloses application of ganoderic acid T and ganoderic acid Me in preparation of inhibitor of tumor growth or proliferation. The internal and external tests show that ganoderic acid T and ganoderic acid Me have obvious action for inhibiting human tumor cell growth or proliferation, at the same time its toxicity for normal cell is low.

Description

Technical field [0001] The invention relates to the use of Ganoderic acid T and Ganoderic acid Me. Background technique [0002] Ganoderma is a precious medicinal fungus with a history of thousands of years of application. The "Shen Nong's Materia Medica" in the Eastern Han Dynasty listed Ganoderma lucidum as a medicine. Later pharmacists of the past dynasties also wrote about Ganoderma lucidum, such as "Baopuzi" by Ge Hong in the Eastern Jin Dynasty, "Xinxiu Materia Medica" by Su Jing of the Tang Dynasty, "Famous Doctors Records" by Tao Hongjing in the Liang Dynasty, and "Compendium of Materia Medica" by Li Shizhen in the Ming Dynasty. Wait. Ancient medical scientists have fully understood the medicinal value of Ganoderma lucidum, and believed that Ganoderma lucidum has the effect of "strengthening the body and strengthening the root" and can be used to treat a variety of diseases. [0003] Modern research on Ganoderma lucidum began in the 1950s. With the success of artificial c...

Claims

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Application Information

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IPC IPC(8): A61K31/575
Inventor 钟建江刘建文唐文
Owner EAST CHINA UNIV OF SCI & TECH
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