44 results about "Sinopodophyllum" patented technology

The invention discloses application of four kinds of known flavonoids compounds to treatment of breast cancers. The four kinds of known flavonoids are known compoundes obtained by separating fruit of sinopodophyllum emodi and have structures shown as structural formulas 1-4 in the specification. Experiments on the effect of the kinds of compounds on inhibiting human breast cancer cells show that the series of compounds have a favorable effect on inhibiting the proliferation of the breast cancer cells.

The invention relates to a preparation method and application of an isopentene alkenylation flavonoid compound with a nerve protection effect. The purposes of preparing the isopentene alkenylation flavonoid compound with the nerve protection effect and preparing medicine for preventing and controlling neurodegenerative diseases are effectively achieved. Fructus podophylli is extracted by ethyl alcohol through heating and refluxing, ethyl alcohol is recycled in a pressure reduction mode and suspended in distilled water, extraction is conducted through petroleum ether, dichloromethane, ethyl acetate and normal butanol, the ethyl acetate extraction part is separated through silica gel column chromatography and eluted through petroleum ether-acetone in a gradient mode, 260 flow parts are collected and combined into 16 components, gel column chromatography and methyl alcohol elution are conducted on the component Fr.2, 35 flow parts are collected, the sub-component Fr.2-1 obtained through combination is eluted through open ODS column chromatography and methyl alcohol-water elution, and elution liquid is collected to obtain sinopodophyllum hexandrum ketone F, sinopodophyllum hexandrum ketone C, sinopodophyllum hexandrum ketone D and sinopodophyllum hexandrum ketone G. The prepared sinopodophyllum hexandrum ketone C, the prepared sinopodophyllum hexandrum ketone D, the prepared sinopodophyllum hexandrum ketone F and the prepared sinopodophyllum hexandrum ketone G have a nerve protection effect.

The invention relates to a method for extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and application of the flavonoid glycoside compounds, and aims to effectively solve application problems of extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and preparing antioxidants and cervical cancer treatment medicines. The flavonoid glycoside compounds are sinoflavonoidgs A and sinoflavonoidgs B, have antioxidation functions, have cytotoxic activity upon human cervical carcinoma cells HeLa, and can be adopted to effectively prepare antioxidants and medicines for preventing human cervical carcinoma cells HeLa. Raw materials of the flavonoid glycoside compounds are rich, the preparation method is easy to operate, the flavonoid glycoside compounds sinoflavonoidgs A and sinoflavonoidgs B can be effectively extracted from dried fruits of sinopodophyllum hexandrum, medicinal values and commercial values of the dried fruitsof sinopodophyllum hexandrum are increased, technical support is provided for preparation of antioxidation medicines and medicines for preventing human cervical carcinoma cells HeLa, and remarkable economic and social benefits can be made.

The invention discloses a polyghace seche date palm bread manufacturing method; the method uses polyghace seche and date palm as main material, and comprises the following steps: raw material pretreatment, traditional Chinese medicine decocted fluid preparation, jam preparation, dough preparation, basic fermentation, secondary fermentation, baking, and the bread is done. Gentiana purdomii marq, white batryticated silkworm, tingli seed, Sinopodophyllum hexandrum, Decaisne fargesii, crab nail, Radix Astragali, CORTEX BERBERIDIS, English walnut seed, Chinese yam, Balanophora harlandii, donkey-hide gelatin and licorice are added; the nutrition value of the polyghace seche and date palm are fully utilized; the raw material and the added traditional Chinese medicine components are combined for synergy, so the bread of the method has basic nutrition of the raw materials, and can provide energy invigorating, moistening lung, relieving cough and resolving phlegm functions, thus benefiting human body; the preparation method is scientific, easy to apply, strong in operationality, and the finished product is soft in mouthfeel and sweet; the date palm aroma fruity is fused so as to provide unique flavor, thus providing obvious economic values.

The invention discloses a Chinese and Western medicine compound caring agent for pathological alopecia and a preparation method of the caring agent. The caring agent mainly consists of the following ingredients: a lombardy poplar extract, an extract of angled bittersweet root, an extract of densehead mountainash fruit, an extract of sinopodophyllum fruit, a russula sanguinaria extract, an extract of tricyrtis macropoda miq., an extract of trapa incisa var. sieb., an extract of glabrousleaf pittosporum root, an extract of Liangshan cordyceps herb, an extract of orchidaceae pecteilis, an extract of nepal fleshspike herb, an extract of urophyllous anotis herb, an extract of fortune paulownia bark, an extract of muskmelon leaves, an extract of trametes robiniphila murr, an extract of bunge bedstraw herb, an extract of radix actinidiae chinensis, propafenone, hesperetin, lycopene, beta-quebrachol, panthenol, an epidermal growth factor, fisetin and magnesium carbonate. The caring agent disclosed by the invention has functions of invigorating spleen and reinforcing stomach, dispelling wind and eliminating dampness, cooling blood and eliminating toxins, nourishing blood and tonifying deficiency, tonifying lung and kidney, promoting hair generation and moistening; the caring agent is capable of taking a conditioning effect from inside to outside, and the caring agent is capable of boosting immunity, relieving pressure and improving blood supply and oxygen supply to brain cells, so as to effectively prevent and treat the pathological alopecia; and the caring agent is good in clinical application effect.

The invention provides a method of producing hair roots of Tibet peaches for podophyllinic acid lactone by gene engineering to convert Tibet peache genes. The invention relates to a method of converting Tibet peache genes with hair root agricillins to gain hair roots of Tibet peaches for podophyllinic acid lactone. Its process is: convert Tibet peache genes with hair root agricillins to gain hair roots of Tibet peaches for podophyllinic acid lactone, ascertain said hair roots by molecule detection, and the results of HPLC detection show that hair roots of Tibet peaches produced by gene convertion could be used to produce podophyllinic acid lactone. The invention offers a lasting new pattern drug-source to the production of podophyllinic acid lactone.

The invention relates to a preparation method of two diflavonoid compounds with antitumor activity in sinopodophyllum emodi and application thereof. The preparation method of the two novel diflavonoidcompounds with the antitumor activity in the sinopodophyllum emodi, namely sinodiflavonoid A and sinodiflavonoid B, comprises the following steps: preparing a crude extract, preliminarily separatingthrough combination of silica gel column chromatography and gel column chromatography, and purifying by high performance liquid chromatography. The preparation method is stable, reliable and high in efficiency; by the preparation method, the two novel diflavonoid compounds with the antitumor activity, namely the sinodiflavonoid A and the sinodiflavonoid B, can be quickly separated from the sinopodophyllum emodi; in addition, results of an activity test show that the sinodiflavonoid A and the sinodiflavonoid B have cytotoxic activity for human cervical cancer cells (HeLa), and can be effectively applied to preparation of medicines for resisting cervical cancer, so that the medicinal value and novel application of the sinopodophyllum emodi are opened up, the commercial value of the sinopodophyllum emodi is increased, and the economic and social benefits are huge.

The invention provides a method for inducing generation of sinopodophyllum emodi somatic embryo, and relates to the technical field of tissue culture. The method comprises the following steps of (1) inducing of sterile test-tube plantlets; (2) differentiating of embryonic callus; (3) inducing of somatic embryo; (4) synchronous regulating and controlling and proliferation culture of the somatic embryo, so as to obtain a large amount of sinopodophyllum emodi somatic embryo clustered shoots. The method has the advantages that the inducing rate of the somatic embryo clustered shoots reaches 75% or above, and the survival rate of regenerated seedlings reaches 85% or above; the operation technology is simple, the limitation by time and seasons is avoided, and the sinopodophyllum emodi somatic embryo regenerated seedlings can be produced indoors in an factory-like way, so as to meet the requirements of production and cultivation.

The invention discloses a whitening and spot-fading composition as well as preparation and use methods thereof. In the whitening and spot-fading composition, a whitening decomposition liquid composition comprises the following raw materials in parts by weight: 1 to 3 parts of radix angelicae sinensis extract, 2 to 4 parts of rhizoma ligustici wallichii extract, 0.9 to 1.1 parts of phellodendron chinense schneid extract, 0.4 to 0.6 part of rhodiola rosea extract, 4 to 6 parts of crocus extract, 1 to 3 parts of saffron extract and 1 to 3 parts of perfume. A whitening traditional Chinese medicinefilm composition comprises the following raw materials in parts by weight: 9 to 11 parts of radix angelicae sinensis, 9 to 11 parts of rhizoma ligustici wallichii, 9 to 11 parts of cortex phellodendri, 9 to 11 parts of radix euphorbiae lantu, 9 to 11 parts of musk, 9 to 11 parts of saffron, 9 to 11 parts of rhodiola rosea and 9 to 11 parts of sinopodophyllum hexandrum. The whitening and spot-fading composition can be directly blended on site for use; transdermal drug delivery is adopted, so that the composition directly reaches the pigment source of the lower layer of the skin after fine permeation to decompose facial pigments, prevent the pigments from flowing back again, rapidly decompose and block the pigments, purify blood pigments of the skin and discharge toxins; and the problems ofstains and darkness of the face of a female at present are fundamentally solved, the effect is obvious, and no side effect and no dependence exist.

The invention relates to a method for identifying podophyllotoxin in sinopodophyllum hexandrum by thin-layer chromatography, and belongs to the technical field of analysis and identification of traditional Chinese medicinal materials. The invention provides a method for identifying podophyllotoxin in podophyllum hexandrum by thin-layer chromatography, which mainly comprises the following steps: preparing a test solution prepared from podophyllum hexandrum rhizomes to be identified and podophyllotoxin into a reference solution, and carrying out thin-layer chromatography; with petroleum ether and acetone with the volume ratio being 3: 2-6: 5 as a developing solvent, carrying out inspection under an ultraviolet lamp with the wavelength being 254 nm to obtain an identification result. The method has the advantages of being safe to operate, convenient to implement and rapid.

The invention specifically relates to an anti-inflammatory bactericidal gynecological gel preparation, characterized by consisting of the following raw materials by weight: 20-30 g of cordyceps sinensis, 20-30 g of sinopodophyllum hexandrum extract, 20-30 g of indigo naturalis powder, 15-25 g of seabuckthorn seed oil, 10-20 g of brucea javanica oil, 10-20 g of artemisia argyi oil, 5-15 g of menthol, 5-10 g of borneol, 5-10 g of methyl cellulose, 5-10 g of agar, and 15-25 g of gum arabic. Natural Chinese herbs are used as raw materials, the formula is obtained by dialectical compatibility, andthe various raw herbs in the ingredients are coordinated with each other, so that viruses and necrotic tissues are selectively cleared, and the regeneration ability of the female vagina is enhanced.

This invention has provided a propagation method for small rhizome of sinopodophyllum hexandrum. The cultivation method of small rhizome of sinopodophyllum hexandrum is established by obtaining sterile embryo, induction of multiple shoots, maturation and segmentation of multiple shoots, growth of seedling, formation and storage of small rhizome. This method is featured with high propagation efficiency, large propagation coefficient, short cultivation cycle, convenience for cultivation, storage and transport, simple operation and low cost, which improves the tissue culture technology of sinopodophyllum hexandrum and provides guarantee for the further expansion of cultivation to sinopodophyllum hexandrum.

The invention discloses a traditional Chinese medicine feed additive suitable for pregnant sows and an application, and belongs to the field of feed additives. The traditional Chinese medicine feed additive consists of the following components in parts by weight: 10-30 parts of radix codonopsis, 10-30 parts of bighead atractylodes rhizomes, 5-20 parts of radix angelicae sinensis, 5-20 parts of common sinopodophyllum fruits, 15-35 parts of glossy privet fruits, 5-15 parts of licorice roots, 1-3 parts of protogen and 2-5 parts of NCG (N-carbamylglutamic acid). The additive is added to a feed according to the weight of the additive being 0.6% of that of the feed at 30 days before birth of the sows, so that the pitocin content and the prolactin content in serum of the sows can be increased, the birth process and the farrowing interval of the sows are shortened, the healthy piglet rate and the average daily gain (ADG) of piglets are increased, the postpartum recovery of the sows is promoted, and effects are significant.