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Method for inducing generation of sinopodophyllum emodi somatic embryo

A technology for somatic embryos and peach seven, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the shortage of peach seed resources, low induction rate, and can not meet the large-scale production of peach seedlings. Production and other problems, to achieve the effect of large-scale artificial cultivation, simple operation process and high embryonicity

Active Publication Date: 2017-11-24
宁夏农林科学院农业生物技术研究中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tissue culture work of Taoerqi has been carried out. Li Mengfei of Gansu Agricultural University and others used the mature zygotic embryos of Taoerqi to directly breed sterile seedlings and obtained Taoerqi aseptic seedlings, but one zygotic embryo can only produce one seedling , the induction rate is low, and the seed resources of Taoerqi are scarce, which cannot meet the large-scale production of Taoerqi seedlings

Method used

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  • Method for inducing generation of sinopodophyllum emodi somatic embryo
  • Method for inducing generation of sinopodophyllum emodi somatic embryo
  • Method for inducing generation of sinopodophyllum emodi somatic embryo

Examples

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Effect test

Embodiment 1

[0052] Take the ripe and plump peach seven seeds of that year, first disinfect the surface with 1% sodium hypochlorite aqueous solution in the ultra-clean workbench for 5 minutes, rinse with sterile water for 3 times, and then disinfect with 70% ethanol for 30 seconds. Rinse 4 times with sterile water, then sterilize with 0.1% mercuric chloride for 5 min, rinse 5 times with sterile water, inoculate the seeds on the pre-cultivated medium, the pre-medium is Ms solid medium, and the sucrose concentration is 30g / L, culture temperature 22±1℃, pre-cultivate for 5 days under dark culture conditions, when the seeds are completely imbibed and no seed contamination is found by visual inspection, take out the pre-cultivated seeds in the ultra-clean workbench, and use a scalpel The umbilical cord cuts the seeds, extrudes the complete embryos, inoculates the embryos in the sterile seedling induction medium, the medium is Ms+GA35.0mg / L+sucrose20g / L+agar powder4.55g / L, the pH value is 5.6, T...

Embodiment 2

[0054] Take the ripe and plump peach seven seeds of that year, first disinfect the surface with 1% sodium hypochlorite aqueous solution in the ultra-clean workbench for 5 minutes, rinse with sterile water for 4 times, then disinfect with 70% ethanol for 30 seconds, and use Rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 5 min, rinse with sterile water for 4 times, inoculate the seeds on the pre-cultivated medium, the pre-medium is Ms solid medium, and the concentration of sucrose is 30g / L, culture temperature 22±1℃, pre-culture for 7 days under dark culture conditions, when the seeds are completely imbibed and no seed contamination is found by visual inspection, take out the pre-cultured seeds in the ultra-clean workbench, and use a scalpel The umbilical cord cuts the seeds, extrudes the complete embryos, inoculates the embryos in the sterile seedling induction medium, the medium is Ms+GA35.0mg / L+sucrose20g / L+agar powder4.55g / L, the pH value...

Embodiment 3

[0056]Take the ripe and plump peach seven seeds of that year, first disinfect the surface with 1% sodium hypochlorite aqueous solution in the ultra-clean workbench for 5 minutes, rinse with sterile water for 4 times, then disinfect with 70% ethanol for 30 seconds, and use Rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 5 min, rinse with sterile water for 4 times, inoculate the seeds on the pre-cultivated medium, the pre-medium is Ms solid medium, and the concentration of sucrose is 30g / L, culture temperature 22±1℃, pre-culture for 7 days under dark culture conditions, when the seeds are completely imbibed and no seed contamination is found by visual inspection, take out the pre-cultured seeds in the ultra-clean workbench, and use a scalpel The umbilical cord cuts the seeds, extrudes the complete embryos, and inoculates the embryos in the sterile seedling induction medium, the medium is Ms+GA35.0mg / L+sucrose20g / L+agar powder4.55g / L, the pH va...

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Abstract

The invention provides a method for inducing generation of sinopodophyllum emodi somatic embryo, and relates to the technical field of tissue culture. The method comprises the following steps of (1) inducing of sterile test-tube plantlets; (2) differentiating of embryonic callus; (3) inducing of somatic embryo; (4) synchronous regulating and controlling and proliferation culture of the somatic embryo, so as to obtain a large amount of sinopodophyllum emodi somatic embryo clustered shoots. The method has the advantages that the inducing rate of the somatic embryo clustered shoots reaches 75% or above, and the survival rate of regenerated seedlings reaches 85% or above; the operation technology is simple, the limitation by time and seasons is avoided, and the sinopodophyllum emodi somatic embryo regenerated seedlings can be produced indoors in an factory-like way, so as to meet the requirements of production and cultivation.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a method for embryogenesis of somatic cells of Taoerqi. Background technique [0002] Sinopodophyllum emodi (Wall.) Ying is a perennial herb of the genus Sinopodophyllum emodi of the Berberidaceae family. It is mainly distributed in the Eastern Himalayas and adjacent areas. It is one of the traditional rare and endangered Chinese medicinal materials in my country. In recent years, studies have found that its roots and rhizomes contain a large number of lignan compounds with anti-cancer activity, among which podophyllotoxin has the highest anti-cancer activity, which is the synthesis of GP7, VP16 and VM The precursor compounds of anticancer drugs such as -26 have very broad application prospects. At present, the medicinal material of Taoerqi mainly depends on wild resources. With the increasing demand, over-excavation has reduced the resources of Taoerqi. [000...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 郭生虎李明陈虞超刘华朱永兴关雅静石磊安钰
Owner 宁夏农林科学院农业生物技术研究中心
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