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A propagation method for small rhizome of sinopodophyllum hexandrum

A technology for rhizome propagation and peach seven, which is applied in the field of agricultural biology to achieve the effects of convenient transportation, improved utilization, and short cultivation period

Active Publication Date: 2017-01-04
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, for the tissue culture of Taoerqi, there is no technology for propagation through small rhizomes.

Method used

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  • A propagation method for small rhizome of sinopodophyllum hexandrum
  • A propagation method for small rhizome of sinopodophyllum hexandrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Obtaining sterile seed embryos.

[0037] Remove moth-eaten and rotten seeds from fresh peach seven seeds, wash them with water, corrode the seed coats with a little concentrated sulfuric acid until the color turns black, soak them in 0.5% sodium carbonate at room temperature for 20 hours to remove oily, waxy and other hydrophobic components, 80mg / L gibberellin (GA4+7) was soaked at 4°C for 28 hours, and 0.2% mercuric chloride was used to disinfect the surface for 6 minutes.

[0038] In the experimental group, the above-mentioned pretreated seeds were blotted dry with sterilized filter paper, and the seed embryos were extruded from the germination hole of the hilum with sterilized needle-nose pliers, and inoculated in MS medium; The pretreatment method of group seeds is the same as above, only the embryos are removed by the following method: the seeds are cut open with a scalpel, and half seeds with embryos are inoculated on MS medium. The two groups were cu...

Embodiment 2

[0041] Example 2: Effects of different hormones on the induction of clustered shoots.

[0042] Use the seed embryo as the explant, and inoculate it in the basal medium under the conditions of red and blue light ratio (3:1), light intensity of 800lux, photoperiod of 14 hours of light / 10 hours of darkness, and corresponding culture temperature of 23 / 18°C (MS, sucrose 50g / L, agar 5g / L, acid hydrolyzed casein 1000mg / L) and add different hormones for culture. See the table below for the ratio of different hormones. The pH of the medium is 6.5.

[0043] hormone 6-BA (mg / L) IAA (mg / L) ZT (mg / L) Experimental results 1# 0.5 0.1 1 After about 20 days, the explants expanded significantly, and the induction rate was 92%. 2# 0.5 0.3 1 After about 13 days, the explants expanded obviously, the induction rate was 92%, and the optimal concentration of IAA 3# 0.5 0.5 1 After about 20 days, the explants expanded significantly, and the induction rate ...

Embodiment 3

[0048] Example 3: The effect of light on the induction of clustered buds.

[0049] The preferred induction medium determined in Example 2 is used to cultivate the seed embryos, change the ratio of red and blue light and the intensity of light, and keep the rest of the culture conditions constant, and carry out the cluster bud induction experiment. The results are as follows:

[0050] hormone 6-BA (mg / L) IAA (mg / L) ZT (mg / L) TDZ (mg / L) Ratio of red and blue light Light intensity (lux) Experimental results 1# 0.5 0.3 1.5 0 3:1 600 Leggy phenomenon in the later stage of culture 2# 0.5 0.3 1.5 0 3:1 800 In the later stage of cultivation, the growth is robust, and the leaves are dark green 3# 0.5 0.3 1.5 0 3:1 1000 In the later stage of cultivation, the growth is robust, and the leaves are dark green 4# 0.5 0.3 1.5 0 3:1 1200 In the later stage of culture, the explants showed dry and brown signs of oxidative dama...

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Abstract

This invention has provided a propagation method for small rhizome of sinopodophyllum hexandrum. The cultivation method of small rhizome of sinopodophyllum hexandrum is established by obtaining sterile embryo, induction of multiple shoots, maturation and segmentation of multiple shoots, growth of seedling, formation and storage of small rhizome. This method is featured with high propagation efficiency, large propagation coefficient, short cultivation cycle, convenience for cultivation, storage and transport, simple operation and low cost, which improves the tissue culture technology of sinopodophyllum hexandrum and provides guarantee for the further expansion of cultivation to sinopodophyllum hexandrum.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for directly propagating small rhizomes in test tubes of Taoerqi by means of cell engineering. Background technique [0002] peach seven ( Sinopodophyllum hexandrum Royle ) is a herbaceous plant belonging to the genus Peach of Berberidaceae, a national third-level protected plant, mainly distributed in western my country. Due to the scarcity and endangerment of natural resources, it has been included in the List of Rare and Endangered Plants in China and the Red Book of Plants in China. [0003] Taoerqi is mainly used in the folk for pain, boils, swollen poison, rheumatic bone pain, tracheitis and other diseases. Modern pharmaceutical research shows that its roots and rhizomes mainly contain lignans, flavonoids, saponins, polysaccharides and other components, of which The lignans had the highest podophyllotoxin content. Podophyllotoxin has a wide range of pha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 杨涛郭琪杨晖张军
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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