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Method for extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and application of flavonoid glycoside compounds

A technology of flavonoid glycosides and compounds, applied in the field of medicine, can solve the problems of no patents or literature reports on biological activity, and achieve the effects of significant economic and social benefits, abundant raw materials, and easy preparation methods.

Inactive Publication Date: 2019-12-10
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The B-ring glycosylated flavone glycosides involved in the present invention were discovered for the first time in plants of the genus Acanthus, and their biological activity has not been reported in patents or literature so far

Method used

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  • Method for extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and application of flavonoid glycoside compounds
  • Method for extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and application of flavonoid glycoside compounds
  • Method for extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and application of flavonoid glycoside compounds

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Embodiment 1

[0021] A method for extracting flavonoid glycosides from Lotus japonicus according to the present invention, using 9 kg of lotus japonicus medicinal material as raw material, heating and refluxing 3 times with 18 L of ethanol with a volume ratio of 95%, the extraction temperature is 95°C, and the extraction time is After 1.5 hours, ethanol was recovered under reduced pressure to obtain extract-like ethanol extract, which was suspended in 3.2L of distilled water, and extracted 3 times with petroleum ether, dichloromethane, ethyl acetate, and n-butanol successively, 3.2L each time, The time is 1.5 hours; the ethyl acetate extraction part is preliminarily separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100:50, 100:70 , 100:100, 100:200, 0:100 petroleum ether-acetone mixed solvent system for gradient elution, each gradient uses 13L eluent, and the flow rate is 15mLmin -1 , every 500ml volume is a fraction, collect 260 fra...

Embodiment 2

[0023]A method for extracting flavonoid glycosides from Lotus japonicus according to the present invention, using 6 kg of lotus japonicus medicinal material as raw material, heating and refluxing 3 times with 30 L of ethanol with a volume ratio of 75%, the extraction temperature is 90° C., and the extraction time is For 2 hours, ethanol was recovered under reduced pressure to obtain extract-like ethanol extract, which was suspended in 2L of distilled water, and extracted three times with petroleum ether, dichloromethane, ethyl acetate, and n-butanol in sequence, 2L each time, for 2 hours; Preliminary separation of ethyl acetate extraction parts by silica gel column chromatography, followed by volume ratios of 100:0, 100:5, 100:7, 100:10, 100:30, 100:50, 100:70, 100 :100, 100:200, 0:100 petroleum ether-acetone mixed solvent system for gradient elution, each gradient uses 9.1L eluent, the flow rate is 10 mLmin -1 , each volume of 350ml is a fraction, collect 260 fractions, and e...

Embodiment 3

[0025] A method for extracting flavonoid glycosides from Lotus japonicus according to the present invention, using 8 kg of lotus japonicus medicinal material as raw material, heating and refluxing 3 times with 24 L of ethanol with a volume ratio of 85%, the extraction temperature is 92° C., and the extraction time each time is After 1.5 hours, ethanol was recovered under reduced pressure to obtain extract-like ethanol extract, which was suspended in 2.8L of distilled water, and then extracted three times with petroleum ether, dichloromethane, ethyl acetate, and n-butanol, each time 2.8L, The time is 1.5 hours; the ethyl acetate extraction part is preliminarily separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100:50, 100:70 , 100:100, 100:200, 0:100 petroleum ether-acetone mixed solvent system for gradient elution, each gradient uses 11.7L eluent, and the flow rate is 13 mLmin -1 , each 450ml volume is a fraction, colle...

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Abstract

The invention relates to a method for extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and application of the flavonoid glycoside compounds, and aims to effectively solve application problems of extracting flavonoid glycoside compounds from dried fruits of sinopodophyllum hexandrum and preparing antioxidants and cervical cancer treatment medicines. The flavonoid glycoside compounds are sinoflavonoidgs A and sinoflavonoidgs B, have antioxidation functions, have cytotoxic activity upon human cervical carcinoma cells HeLa, and can be adopted to effectively prepare antioxidants and medicines for preventing human cervical carcinoma cells HeLa. Raw materials of the flavonoid glycoside compounds are rich, the preparation method is easy to operate, the flavonoid glycoside compounds sinoflavonoidgs A and sinoflavonoidgs B can be effectively extracted from dried fruits of sinopodophyllum hexandrum, medicinal values and commercial values of the dried fruitsof sinopodophyllum hexandrum are increased, technical support is provided for preparation of antioxidation medicines and medicines for preventing human cervical carcinoma cells HeLa, and remarkable economic and social benefits can be made.

Description

technical field [0001] The invention relates to medicine, in particular to a method for extracting flavonoid glycosides from lotus lobata and its application. Background technique [0002] Oxidative stress means that the active oxygen free radicals produced in body tissues or cells exceed the original ability to use or scavenge oxygen free radicals, and the redox balance is destroyed, resulting in excessive accumulation of active oxygen free radicals and their Related metabolites cause certain damage to the body, and thus present a certain pathological state. Oxidative stress is believed to be the cause of many degenerative diseases such as cancer, chronic inflammation, cardiovascular disease, neurological diseases, diabetes, Alzheimer's and Parkinson's diseases, etc. Therefore, inhibiting oxidative stress damage has become one of the important ways to treat diseases related to oxidative stress. [0003] Malignant tumor is a common and frequently-occurring disease that ser...

Claims

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Application Information

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IPC IPC(8): C07H15/26C07H1/08A61P35/00
CPCA61P35/00C07H1/08C07H15/26
Inventor 孙彦君冯卫生陈辉韩红艳王俊敏郝志友张艳丽陈豪杰赵晨韩瑞杰
Owner HENAN UNIV OF CHINESE MEDICINE
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