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Preparing method and application of two isopentene-base biflavones podoverine B and podoverine C in sinopodophyllum hexandrum

A technology of isopentenyl and peach seven is applied in the field of medicine to achieve the effect of high yield, high purity and short time

Inactive Publication Date: 2016-03-02
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method of two prenylated bisflavonoids podoverineB and podoverineC in Taoerqi, which can effectively solve the problem of rapid extraction from Taoerqi Problems in the Preparation of Two Prenylated Biflavonoids

Method used

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  • Preparing method and application of two isopentene-base biflavones podoverine B and podoverine C in sinopodophyllum hexandrum
  • Preparing method and application of two isopentene-base biflavones podoverine B and podoverine C in sinopodophyllum hexandrum
  • Preparing method and application of two isopentene-base biflavones podoverine B and podoverine C in sinopodophyllum hexandrum

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Experimental program
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Effect test

Embodiment 1

[0015] The preparation method of two prenylated bisflavonoids podoverineB and podoverineC separated from Taoerqi is realized by the following method:

[0016] 1), the preparation of crude extract: pulverize 3kg of Peach chinensis into the coarse powder of 40 mesh sieves, use 95% ethanol 90 ℃ of reflux extraction 3 times with the volume concentration of 12 times of weight of Peach chinensis, obtain ethanol extract , 1.5 hours each time, combine the ethanol extracts, concentrate under reduced pressure until there is no alcohol smell, to obtain a concentrate, add 12 times the weight of the concentrate to suspend in distilled water to obtain a suspension, add an equal volume of ethyl acetate to the suspension to extract 3 times, the ethyl acetate extraction layer was combined, the solvent was recovered under reduced pressure, and the extract of the ethyl acetate extraction part was obtained, which was the crude extract of Taoerqi;

[0017] 2) Preliminary separation by gel column c...

Embodiment 2

[0020] The preparation method of two prenylated bisflavonoids podoverineB and podoverineC separated from Taoerqi is realized by the following method:

[0021]1), the preparation of crude extract: pulverize 5 kg of Peach chinensis into coarse powder of 40 mesh sieves, use 95% ethanol with a volume concentration of 10 times the weight of Peach chinensis to reflux and extract twice at 95° C. to obtain an ethanol extract , 2 hours each time, combine the ethanol extracts, concentrate under reduced pressure until there is no alcohol smell, to obtain a concentrate, add 10 times the weight of the concentrate to suspend in distilled water to obtain a suspension, add an equal volume of ethyl acetate to the suspension to extract 3 times, the ethyl acetate extraction layer was combined, the solvent was recovered under reduced pressure, and the extract of the ethyl acetate extraction part was obtained, which was the crude extract of Taoerqi;

[0022] 2) Preliminary separation by gel column...

Embodiment 3

[0025] The preparation method of two prenylated bisflavonoids podoverineB and podoverineC separated from Taoerqi is realized by the following method:

[0026] 1), preparation of crude extract: Pulverize 6kg of Peach Seven into a coarse powder of 40 mesh sieves, use 95% ethanol with a volume concentration of 8 times the weight of Peach Pine to reflux extract for 3 times at 92°C to obtain an ethanol extract , 1.5 hours each time, combine the ethanol extracts, concentrate under reduced pressure until there is no alcohol smell, to obtain a concentrate, add 8 times the weight of the concentrate to suspend in distilled water to obtain a suspension, add an equal volume of ethyl acetate to the suspension to extract 3 times, the ethyl acetate extraction layer was combined, the solvent was recovered under reduced pressure, and the extract of the ethyl acetate extraction part was obtained, which was the crude extract of Taoerqi;

[0027] 2) Preliminary separation by gel column chromatogr...

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Abstract

The invention relates to a preparing method of two isopentene-base biflavone compounds podoverine B and podoverine C in sinopodophyllum hexandrum to effectively and quickly prepare the two isopentene-base biflavone compounds from sinopodophyllum hexandrum. The method comprises the steps of smashing sinopodophyllum hexandrum into coarse power, conducting ethanol-reflux extraction, conducting vacuum concentration on ethanol extraction liquid till no alcohol taste exists, and conducting ethyl acetate extraction to generate sinopodophyllum hexandrum crude extract; conducting gel column chromatography primary separation on the sinopodophyllum hexandrum crude extract, and then conducting high-speed counter-current chromatography purification to generate the two isopentene-base biflavone compounds podoverine B and podoverine C. The method is stable, reliable, high in efficiency, short in time and high in yield, the two isopentene-base biflavone compounds can be obtained from sinopodophyllum hexandrum quickly through separation, and purity is high. Activity tests show that the two isopentene-base biflavone compounds have cytotoxic activity on the prostate cancer cell PC-3, can be effectively used for preparing drugs for treating prostatic cancer, and can generate remarkable economic and social benefits.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of two prenylated diflavones podoverine B and podoverine C in Taoerqi. Background technique [0002] Malignant tumor is a common and frequently-occurring disease that seriously threatens human health and life. Today, cancer is the leading cause of death worldwide. Prostate cancer is one of the most common malignancies in men, with the highest incidence among malignancies among adult males in the United States and Europe. With the gradual improvement of people's living conditions, the continuous extension of life expectancy and the great changes in dietary structure, the incidence and mortality of prostate cancer in my country are gradually increasing, and most of them are in the middle and late stage when they are discovered, about 80% of the patients were first found to have distant metastatic lesions, and then they were diagnosed with prostate cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40A61K31/352A61P35/00A61P13/08
CPCC07D311/30C07D311/40
Inventor 孙彦君宋军营高美玲王俊敏张艳丽裴莉昕纪宝玉王凯波孙印石
Owner HENAN UNIV OF CHINESE MEDICINE
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