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Method for identifying podophyllotoxin in sinopodophyllum hexandrum by thin-layer chromatography

A technology of podophyllotoxin and thin-layer chromatography, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of potential safety hazards, strong irritation, troublesome operation, etc., and achieve safe operation, simple preparation method, and optimized color development conditions Effect

Pending Publication Date: 2021-03-23
CHONGQING THREE GORGES MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, although chloroform and methanol are non-genotoxic in the developing agent used, because there are second-class solvents that are carcinogenic to animals, and the developing agent needs to be allowed to stand before being layered to remove the lower layer, the operation is cumbersome; The sulfuric acid contained in the color developer has strong corrosiveness, and 10% sulfuric acid ethanol has strong irritation when used; at the same time, after color development, the solution needs to be heated to a temperature of 105 ° C, and the color development method is also more complex. Troublesome and a safety hazard

Method used

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  • Method for identifying podophyllotoxin in sinopodophyllum hexandrum by thin-layer chromatography
  • Method for identifying podophyllotoxin in sinopodophyllum hexandrum by thin-layer chromatography
  • Method for identifying podophyllotoxin in sinopodophyllum hexandrum by thin-layer chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method of identifying a podium toxin in Taozi seven, specifically, according to the following operational steps:

[0037] First, select 6 batch of dry different peach seven stems into 6 supply solutions (budget as G1, G2, G3, G4, G5 and G6), and podophyllotoxin formulated into a control solution (a brief note D1), the volume ratio of 3: 2 petroleum ether and acetone is an expander:

[0038] The peaches of the identified peaches were taken. After 60 mesh sieve samples, precision weighted 0.5 g, was added to 10 ml of methanol, and sonicated for 20 min. After filtration, the filtrate was evaporated, and the obtained residue added methanol Make full dissolution can be made into a test solution;

[0039] According to 0.5: 1, mg: ml mass volume is soluble in the methanol solvent in a methanol solvent, which can be fully dissolved in the methanol solvent.

[0040] Oil ethers and acetone are mixed in accordance with the volume ratio of 3: 2 to form an expander.

[0041] Next, eac...

Embodiment 2

[0047] The conditions deployed in Example 1 were turned from a refrigerator having a temperature of 25 ° C and a humidity of 60% to a refrigerator having a temperature of 4 ° C, and the resulting thin layer was viewed under ultraviolet light having a wavelength of 254 nm figure 2 As shown, it can also identify the peaches of the peaches to be identified.

Embodiment 3

[0049] The conditions deployed in Example 1 were changed from a temperature of 25 ° C, a humidity of 60% to a temperature of 30 ° C, a humidity of 60%, and the thin layer obtained under an ultraviolet light having a wavelength of 254 nm. image 3 As shown, it can also identify the peaches of the peaches to be identified.

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Abstract

The invention relates to a method for identifying podophyllotoxin in sinopodophyllum hexandrum by thin-layer chromatography, and belongs to the technical field of analysis and identification of traditional Chinese medicinal materials. The invention provides a method for identifying podophyllotoxin in podophyllum hexandrum by thin-layer chromatography, which mainly comprises the following steps: preparing a test solution prepared from podophyllum hexandrum rhizomes to be identified and podophyllotoxin into a reference solution, and carrying out thin-layer chromatography; with petroleum ether and acetone with the volume ratio being 3: 2-6: 5 as a developing solvent, carrying out inspection under an ultraviolet lamp with the wavelength being 254 nm to obtain an identification result. The method has the advantages of being safe to operate, convenient to implement and rapid.

Description

Technical field [0001] The present invention belongs to the technical field of Chinese herbal medicinal analysis and identification, and more particularly to a method of identifying podium toxins in peach seven-in-chromatography. Background technique [0002] Sinopodophyllum HEXANDRUM (ROYLE) is a seven-child herbal plant in XiaodoDophellum, mainly distributed in my country Shaanxi, Tibet, Yunnan, Sichuan, Gansu, etc., for III protection species. The 2015 version of "China Pharmacopoeia" is a commonly used Tibetan medicine for the dry fruit of the peaches seven. Peach seven stems have a long history of private medication, and "Shennong Herb" is recorded in the name "ghost", mainly for bruises, rheumatoid bones, snake bites. Modern pharmacological studies have shown that the active ingredient in Taozi Seven is mainly ambigine-like compound, which has high efficiency anti-tumor activity, of which ghost white toxin is used as a synthetic anti-cancer drug VP-16, VM-26, GP7, NK611 and...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 邹妍琳黄倩谭小蓉谭小燕朱小龙
Owner CHONGQING THREE GORGES MEDICAL COLLEGE
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