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Preparation method of two diflavonoid compounds with antitumor activity in sinopodophyllum emodi and application thereof

A technology with anti-tumor activity and biflavonoids, which is applied in the field of medicine to achieve the effect of increasing commercial value, huge economic and social benefits, and high efficiency

Pending Publication Date: 2018-06-08
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bisflavonoids Sinodiflavonoid A and Sinodiflavonoid B involved in the present invention have not been reported in relevant literature at home and abroad so far

Method used

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  • Preparation method of two diflavonoid compounds with antitumor activity in sinopodophyllum emodi and application thereof
  • Preparation method of two diflavonoid compounds with antitumor activity in sinopodophyllum emodi and application thereof
  • Preparation method of two diflavonoid compounds with antitumor activity in sinopodophyllum emodi and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0016] In the specific implementation of the present invention, the preparation method of two biflavonoids with anti-tumor activity in a kind of Taoerqi comprises the following steps:

[0017] 1), prepare the crude extract: 10.57kg of the medicinal materials of P. chinensis collected from Kangding County, Sichuan are pulverized and passed through a 40-mesh sieve to obtain coarse powder, and the coarse powder is 95% ethanol with a volume concentration of 8 times the weight of P. chinensis medicinal materials. Reflux extraction 3 times, 1.5 hours each time, the extraction temperature is 95 ° C, the ethanol extracts are combined, concentrated under reduced pressure to a concentrate without alcohol taste, add 8 times the weight of the concentrate to suspend in distilled water, and then add the suspension in turn. An equal volume of ethyl acetate was extracted, and the solvent was recovered under reduced pressure at 45° C. to obtain the extract of the ethyl acetate extraction part, ...

Embodiment 2

[0021] In the specific implementation of the present invention, it can also be given by the following examples, the preparation method of two biflavonoids with antitumor activity in a kind of Taoerqi, comprising the following steps:

[0022] 1), preparation of crude extract: 10.08 kg of the medicinal materials collected from Datong County, Qinghai are pulverized and passed through a 40-mesh sieve to obtain coarse powder, and the coarse powder is refluxed with 95% ethanol at a volume concentration of 10 times the weight of the medicinal materials of Radix Peach chinensis. Extract 3 times, 1 h each time, the extraction temperature is 90 °C, combine the ethanol extracts, concentrate under reduced pressure until there is no alcohol smell, add 10 times the weight of the concentrate to suspend in distilled water, and sequentially add the same volume of ethyl acetate as the suspension. Ester extraction, the solvent was recovered under reduced pressure at 45°C, and the extract of the e...

Embodiment 3

[0026] In the specific implementation of the present invention, it can also be given by the following examples, the preparation method of two biflavonoids with antitumor activity in a kind of Taoerqi, comprising the following steps:

[0027] 1), preparation of crude extract: 10.69kg of the medicinal materials collected from Yongdeng County, Gansu, and 10.69 kg of Radix chinensis are pulverized and passed through a 40-mesh sieve to obtain coarse powder, which is 95% ethanol with a volume concentration of 12 times the weight of Radix Root. Reflux extraction 3 times, 1 h each time, at 90 °C, combine the ethanol extracts, concentrate under reduced pressure to a concentrated solution without alcohol smell, add 12 times the weight of the concentrated solution to suspend in distilled water, and then add the ethanol and the suspension in turn. An equal volume of ethyl acetate was extracted, and the solvent was recovered under reduced pressure at 45° C. to obtain the extract of the ethy...

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Abstract

The invention relates to a preparation method of two diflavonoid compounds with antitumor activity in sinopodophyllum emodi and application thereof. The preparation method of the two novel diflavonoidcompounds with the antitumor activity in the sinopodophyllum emodi, namely sinodiflavonoid A and sinodiflavonoid B, comprises the following steps: preparing a crude extract, preliminarily separatingthrough combination of silica gel column chromatography and gel column chromatography, and purifying by high performance liquid chromatography. The preparation method is stable, reliable and high in efficiency; by the preparation method, the two novel diflavonoid compounds with the antitumor activity, namely the sinodiflavonoid A and the sinodiflavonoid B, can be quickly separated from the sinopodophyllum emodi; in addition, results of an activity test show that the sinodiflavonoid A and the sinodiflavonoid B have cytotoxic activity for human cervical cancer cells (HeLa), and can be effectively applied to preparation of medicines for resisting cervical cancer, so that the medicinal value and novel application of the sinopodophyllum emodi are opened up, the commercial value of the sinopodophyllum emodi is increased, and the economic and social benefits are huge.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method and application of two biflavonoid compounds with antitumor activity in Taoerqi. Background technique [0002] Malignant tumor is a common and frequently-occurring disease that seriously threatens human health and life. Today, cancer has become the leading cause of death worldwide. Synthetic antitumor drugs widely used in clinical practice generally have strong side effects and poor selectivity. Chinese herbal medicine has a long history of application in anti-tumor. It is the primary problem for pharmaceutical researchers to find high-efficiency and low-toxic anti-tumor active substances from Chinese herbal medicine and develop new anti-tumor drugs with strong selectivity and low toxicity. [0003] Taoerqi is the dry root and rhizome of Sinopodophyllum emodi (Wall.) Ying. Guiji is a medicinal plant with a long history. It is recorded in the ancient "Shen Nong's Materia Medica"...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61P35/00
CPCC07D311/62
Inventor 孙彦君冯卫生高美玲付露弓建红时博张艳丽陈辉李晓坤郝志友
Owner HENAN UNIV OF CHINESE MEDICINE
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