DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid
A technology of mesenchymal stem cells and injection, applied in the field of cell biology, can solve the problems of serum culture affecting mesenchymal stem cells inducing differentiation function, increasing the risk of exogenous protein rejection, increasing the risk, etc., and achieving good differentiation ability, reducing the risk of contamination, ensuring the effect of differentiation ability
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[0038]The second aspect of the present invention provides a method for preparing a DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation liquid, comprising the following steps:
[0039] S1. According to the formula, add dextran 40 glucose injection to a 500mL receiver, then slowly add compound electrolyte injection, sodium chloride injection, glucose injection, mesenchymal stem cell serum-free medium, Shake sideways until mixed;
[0040] S2. Then slowly add the formulated amount of human serum albumin, and mix upside down to obtain the DMSO-free human umbilical cord mesenchymal stem cell injection cryopreservation solution.
[0041] When the preparation raw material also includes a serum substitute, the preparation method includes the following steps:
[0042] S1. According to the formula, add dextran 40 glucose injection to a 500mL receiver, and then slowly add compound electrolyte injection, sodium chloride injection, glucose injection, mesenchymal...
Embodiment 1
[0055] Example 1 provides a DMSO-free human umbilical cord mesenchymal stem cell injection frozen storage solution, in parts by volume, its preparation raw materials include 55 parts of compound electrolyte injection, 30 parts of dextran 40 glucose injection, sodium chloride 5 parts of injection, 5 parts of glucose injection, 40 parts of human serum albumin; 6 parts of serum-free medium for mesenchymal stem cells.
[0056] The compound electrolyte injection was purchased from Hebei Tiancheng Pharmaceutical Co., Ltd.;
[0057] The dextran 40 glucose injection was purchased from Sichuan Kelun Pharmaceutical Co., Ltd.;
[0058] The sodium chloride injection was purchased from Guangdong Otsuka Pharmaceutical Co., Ltd.;
[0059] The glucose injection was purchased from Jimin Health Management Co., Ltd.;
[0060] The human albumin was purchased from Octapharma AB;
[0061] The serum-free medium for mesenchymal stem cells was purchased from Wuhan Punuosai Life Science and Technolo...
Embodiment 2
[0070] Example 2 provides a DMSO-free human umbilical cord mesenchymal stem cell injection frozen storage solution, in parts by volume, its preparation raw materials include 55 parts of compound electrolyte injection, 30 parts of dextran 40 glucose injection, sodium chloride 5 parts of injection, 5 parts of glucose injection, 40 parts of human serum albumin; 6 parts of serum-free medium for mesenchymal stem cells, 2 parts of serum substitute.
[0071] The compound electrolyte injection was purchased from Hebei Tiancheng Pharmaceutical Co., Ltd.;
[0072] The dextran 40 glucose injection was purchased from Sichuan Kelun Pharmaceutical Co., Ltd.;
[0073] The sodium chloride injection was purchased from Guangdong Otsuka Pharmaceutical Co., Ltd.;
[0074] The glucose injection was purchased from Jimin Health Management Co., Ltd.;
[0075] The human albumin was purchased from Octapharma AB;
[0076] The serum-free medium for mesenchymal stem cells was purchased from Wuhan Punuo...
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