Tissue preserving fluid for treating HIV (human immunodeficiency virus) infection-accompanied colorectal cancer and decompensated cirrhosis complicated small liver carcinoma

Abstract

The invention discloses a tissue preserving fluid. The tissue preserving fluid is composed of, by concentration, 1.0-2.5%w / v of poloamer, 1.0-2.5%w / v of polyvinylpyrrolidone, 0.2-1.0%w / v of glucose, 15-40%w / v of compound amino acid injection, 5-30%w / v of propylene glycol, 10-40%w / v of PBS (phosphate buffer saline) vitamin E, 0.1-1.5%w / v of vitamin C and 0.1-1.0%w / v of aqueous solvent for injection. Due to the fact of containing no DMSO (dimethylsulfoxide) or protein, the tissue preserving fluid can avoid toxicity produced due to DMSO reverse infusion as well as safety worries of serum and proteins on clinical application, thereby being high in safety.

Description

technical field [0001] The invention relates to the technical field of cryopreservation of bone marrow tissue and bone marrow transplantation, in particular to a tissue cryopreservation solution and its preparation method and clinical application. Background technique [0002] Stem cells are primitive cells with self-replication and multi-directional differentiation potential, which can form various tissues and organs of the human body. In recent years, research on the use of stem cells to treat diseases has become a hot spot. Stem cell technology is the cutting-edge technology of biological treatment, known as regenerative medicine. In stem cell therapy technology, the supply of high-quality and large-scale stem cells is the key and also a difficult problem. Bone marrow is the hematopoietic tissue of the human body. It is located in many bones of the body. It contains various cells such as hematopoietic stem cells and mesenchymal stem cells, and is one of the main sources o...

AI Technical Summary

Problems solved by technology

[0011] There are many disadvantages in the use of serum. First, due to the complex composition of serum, the proteins, factors, hormones and other components contained in it are uncertain, which will interfere with the differentiation of stem cells. For example, rat neural stem cells cultured without serum have more Differentiate into neurons, while those cultured with serum are more likely to differentiate into astrocytes and oligodendrocytes. The effects of serum on the proliferation and differentiation of stem cells derived from cord blood and bone marrow have also been reported frequently; It is believed that stem cells will endocytose serum into cells, which may cause changes in the expression of stem cell proteins and cause an immune response to heterologous proteins; third, the factors and hormones contained in serum will change the adhesion of cells, which may change the expression of surface substances on cells , affecting the characteristics of stem cells; Fourth, our laboratory found that after the mesenchymal stem cells cultured with serum were replaced with serum-free medium, serum residues could still be detected after two generations of culture, which shows that serum has a great influence on stem cells

Coupled with the shortcomings of unstable serum quality and high price, serum-free cryopreservation and resuscitation technology has become a development trend

[0012] Studies have shown that DMSO has cytotoxicity, interacts with protein hydrophobic groups, and causes protein denaturation. It has vascular toxicity and liver and kidney toxicity. The safety of direct reinfusion into the human body is not good, which is not conducive to clinical application.

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