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Rapid detection method of methionine sulfoxide in amino acid injection

A methionine sulfoxide and detection method technology, applied in the field of rapid detection of methionine sulfoxide in amino acid injections, can solve the problem of large damage to the chromatographic column and liquid phase system, susceptibility to derivative reagents and negative interference, poor peak shape and resolution and other problems, to achieve the effect of large reagent consumption, low reagent consumption, and reduced detection time

Active Publication Date: 2017-06-13
湖北华仁同济药业有限责任公司
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Problems solved by technology

[0004] The technical problem solved by the present invention is that in the prior art, pre-derivatization is used to detect methionine sulfoxide in the sample, the peak shape and resolution are poor, and it is easy to be interfered by derivatization reagents and negatives, and the damage to the chromatographic column and the liquid phase system is large; and Determination of methionine sulfoxide in gelatin by the old machine L-8500 amino acid analyzer such as unsatisfactory repeatability and long time consumption

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  • Rapid detection method of methionine sulfoxide in amino acid injection
  • Rapid detection method of methionine sulfoxide in amino acid injection
  • Rapid detection method of methionine sulfoxide in amino acid injection

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Embodiment 1

[0027] A rapid detection method for methionine sulfoxide in amino acid injection, comprising the following steps:

[0028] Precisely measure 1.00ml of this product, place it in a 10ml measuring bottle, add 0.02mol / L hydrochloric acid solution to make the test solution, take 20μl and inject it into the amino acid analyzer, detect it with the amino acid automatic analyzer, and record the chromatogram; An appropriate amount of methionine sulfoxide reference substance was accurately weighed, and 0.02 mol / L hydrochloric acid solution was added to make a solution containing methionine sulfoxide with a concentration of 5 μg / ml. The same method was used for determination, and the external standard method was used to calculate the peak area.

[0029] The mobile phases used when the amino acid automatic analyzer is used for detection are B1 and B6, and their compositions are shown in the following table:

[0030]

[0031] Used derivatization reaction reagents R1, R2, R3, its composit...

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Abstract

The invention belongs to the technical filed of a drug detection method and particularly relates to a rapid detection method of methionine sulfoxide in amino acid injection. An automatic amino acid analyzer is adopted for detection; only two mobile phases are adopted for separation, R1-R3 are derivative reaction reagents, Hitachi special ion exchange resin is taken as a filling agent, and the calculation is performed by peak area according to an external standard method. Through contrastive study, as a primary standard procedure adopts six mobile phases, the consumption of the reagents is high, and the mobile phases are various. As the two mobile phases are adopted, the consumption of the reagents is low, and a spectrogram is briefer. The test of the primary standard procedure needs 53.8 minutes, and the detection method provided by the invention only needs 29.5 minutes, so that the detection time is greatly reduced, and the efficiency is higher.

Description

technical field [0001] The invention belongs to the technical field of drug detection methods, and in particular relates to a rapid detection method for methionine sulfoxide in amino acid injections. Background technique [0002] The human body needs to take in a variety of amino acids to maintain life activities, and some patients who need nutritional supplements usually need clinical enteral and parenteral nutritional support. Methionine is one of the essential amino acids that constitute the human body, participates in protein synthesis, and is one of the main components in amino acid injections. The molecular formula of methionine contains a sulfhydryl group, which is reductive and easily oxidized and degraded into methionine sulfoxide. In the import registration standard of medium and long-chain fat emulsion / amino acid (16) / glucose (16%) injection (Braun Medical Company, standard number: JX20110281), pre-column derivatization is used to detect methionine sulfoxide in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 尹冬丽韩瑶
Owner 湖北华仁同济药业有限责任公司
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