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L-lysine fermenting method

A technology of lysine fermentation and fermentation method, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problem of low end-point lysine content, total acid content and sugar-acid conversion rate, and high production cost. The problem of high level is not inferior to achieve the effect of improving the content of lysine at the end point

Inactive Publication Date: 2014-12-31
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the end-point lysine content, total acid content and sugar-acid conversion rate of the current lysine preparation method are low, and the production cost remains high.

Method used

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  • L-lysine fermenting method

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Embodiment 1

[0039] The fermentation method of L-lysine in this embodiment includes three steps of lysine primary seed cultivation, lysine secondary seed cultivation and lysine fermentation cultivation. The specific operation of each step is as follows:

[0040] 1. Primary seed culture of lysine

[0041] Prepare lysine primary seed medium. Lysine primary seed culture medium comprises sucrose 20g / L in the present embodiment, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 1.5g / L, ammonium chloride 9g / L, yeast extract powder 5g / L, threonine Acid 0.5g / L, methionine 0.5g / L, monosodium glutamate 7g / L, sodium pyruvate 0.5g / L.

[0042] With the prepared lysine first-grade seed culture medium, adjust the pH value to 6.0 with 20% (volume ratio) potassium hydroxide solution, pass steam to heat up the culture medium to 121 ° C, sterilize for 20 minutes, and turn on the circulating water to cool down to 36°C and kept constant. Start stirring at 300rpm, introduce sterile air according to t...

Embodiment 2

[0052] The fermentation method of L-lysine in this embodiment includes three steps of lysine primary seed cultivation, lysine secondary seed cultivation and lysine fermentation cultivation. The specific operation of each step is as follows:

[0053] 1. Primary seed culture of lysine

[0054] Prepare lysine primary seed medium. Lysine primary seed culture medium comprises sucrose 40g / L in the present embodiment, magnesium sulfate 1.5g / L, potassium dihydrogen phosphate 1.5g / L, ammonium chloride 10g / L, yeast extract powder 8g / L, threonine Acid 0.4g / L, methionine 0.4g / L, monosodium glutamate 9g / L, sodium pyruvate 0.6g / L.

[0055] Adjust the prepared primary lysine seed medium to pH 6.0 with 20% potassium hydroxide, heat the medium to 121°C with steam, sterilize it for 20 minutes, and cool it down to 37°C with circulating water and keep it constant. Start stirring at 350rpm, introduce sterile air according to the ventilation ratio of 1:0.5, adjust the tank pressure to 0.05Mpa, a...

Embodiment 3

[0065] The fermentation method of L-lysine in this embodiment includes three steps of lysine primary seed cultivation, lysine secondary seed cultivation and lysine fermentation cultivation. The specific operation of each step is as follows:

[0066] 1. Primary seed culture of lysine

[0067] Prepare lysine primary seed medium. Lysine primary seed culture medium comprises sucrose 35g / L in the present embodiment, magnesium sulfate 0.7g / L, potassium dihydrogen phosphate 0.7g / L, ammonium chloride 11g / L, yeast extract powder 7g / L, threonine Acid 0.6g / L, methionine 0.46g / L, monosodium glutamate 7g / L, sodium pyruvate 0.5g / L.

[0068] The prepared primary lysine seed medium was adjusted to pH 6.3 with 20% potassium hydroxide, and the medium was heated to 121°C by steaming, sterilized for 20 minutes, cooled to 37°C with circulating water and kept constant. Start stirring at 300rpm, introduce sterile air according to the ventilation ratio of 1:0.6, adjust the tank pressure to 0.06Mpa...

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Abstract

The invention relates to the field of fermentation, in particular to an L-lysine fermenting method. The L-lysine fermenting method comprises the following steps: inoculating a lysine first stage seed medium with a lysine shake flask seed, culturing the seed till the seed is mature, then, inoculating the seed in a lysine second stage seed medium for culturing till the seed reaches a mature standard of a lysine second stage seed, inoculating a lysine fermenting medium with the second stage seed, and under conditions of a fed-batch carbon source and a fed-batch nitrogen source, performing fermentation culture till the end of fermentation, wherein the nitrogen source in the lysine primary seed medium, the lysine secondary seed medium and the lysine fermenting medium is ammonium chloride, and during fermentation culture, the fed-batch nitrogen source is an ammonium chloride solution. By the L-lysine fermenting method, the final content of lysine, the total acid amount and the conversion ratio of saccharic acid can be significantly increased.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a fermentation method of L-lysine. Background technique [0002] L-lysine is a basic essential amino acid, and it is also the most important of the eight essential amino acids that cannot be synthesized by humans and animals, so it is often called the first limiting amino acid. The human body must obtain lysine through daily diet and supplements, but because the content of lysine in food is very low, and it is easily destroyed during processing, resulting in lysine deficiency. [0003] L-Lysine is widely used in pharmaceutical industry, food industry, animal feed and other fields. Adding a certain amount of L-lysine to cereal-based foods can increase the absorption rate and nutritional value of its protein; adding L-lysine to feed can improve the utilization rate of feed; in the pharmaceutical industry, L -Lysine is generally used as an amino acid infusion, and can be used as a drug...

Claims

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Application Information

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IPC IPC(8): C12P13/08C12R1/19C12R1/13C12R1/15
Inventor 尚海涛徐斌杨为华邓远德张家泉张雪锋穆晓玲
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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