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Production method of lysine

A production method and lysine technology, which are applied in the production field of lysine, can solve the problem of low lysine production capacity, etc., and achieve the effects of improving the lysine content at the end point, improving economic benefits and improving production capacity.

Active Publication Date: 2014-12-24
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to provide a new production method of lysine to overcome the defect that the production capacity of lysine is not high in the prior art

Method used

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  • Production method of lysine

Examples

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Effect test

Embodiment 1

[0071] This example is used to illustrate the production method of lysine provided by the present invention.

[0072] (1) preparation of seed tank culture medium, concrete composition is: relative to every liter of culture medium, the consumption of getting the liquefied supernatant of starchy raw materials is 35 grams, the consumption of corn steep liquor (35% by weight) is 80 grams, phosphoric acid The consumption of dipotassium hydrogen is 1.0 grams, the consumption of magnesium sulfate is 0.5 grams, the consumption of ammonium sulfate is 10 grams, the consumption of threonine is 0.2 grams, the consumption of methionine is 0.2 grams, the consumption of glutamic acid is 0.2 grams, biological The consumption of vitamin B1 is 0.01 mg, the consumption of nicotinamide is 0.1 mg, and the consumption of vitamin B1 is 0.2 mg. Heat the medium to 121°C for disinfection, maintain it for 20 minutes, then cool down to 37°C and keep it constant. Turn on the stirring, adjust the tank pre...

Embodiment 2

[0077] This example is used to illustrate the production method of lysine provided by the present invention.

[0078] The formulation of the medium for the seed tank, the culture method for the mature seed liquid of the seed tank, and the formulation of the medium for the fermenter are all the same as in Example 1.

[0079] The medium volume in the fermentor was 40% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 12% by volume based on the inoculated fermentation medium. After the inoculation, the starchy raw material liquefied clear liquid and ammonium sulfate were fed continuously, and the amount of the starchy raw material liquefied clear liquid obtained by feeding was added so that the concentration of reducing sugar in the fermentation broth was controlled at 5-7 grams per liter. Add the amount of ammonium sulfate to control the concentrati...

Embodiment 3

[0082] This example is used to illustrate the production method of lysine provided by the present invention.

[0083] The formulation of the medium for the seed tank, the culture method for the mature seed liquid of the seed tank, and the formulation of the medium for the fermenter are all the same as in Example 1.

[0084]The medium volume in the fermentor was 60% of the fermentor volume. The mature seed liquid is inserted into the culture medium of the fermenter for fermentation and cultivation, and the inoculum amount of the seed liquid is 18% by volume based on the inoculated fermentation medium. After the inoculation, the starchy raw material liquefied clear liquid and ammonium sulfate were fed continuously, and the amount of the starchy raw material liquefied clear liquid obtained by feeding was added so that the concentration of reducing sugar in the fermentation broth was controlled at 8-10 grams per liter. The amount of ammonium sulfate added is to control the concen...

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Abstract

The invention discloses a production method of lysine. The method comprises the following step: inoculating a lysine fermentation strain into a lysine fermentation culture medium for fermentation culture under the condition of feeding a carbon source and a nitrogen source, and is characterized by feeding an accelerant into a fermentation liquid in a period from 2-6 hours of fermentation culture to 12-16 hours of fermentation culture; feeding an inorganic salt into the fermentation liquid in a period from 12-16 hours of fermentation culture to 6-8 hours before fermentation culture is finished; feeding amino acids into the fermentation liquid in a period from 16-20 hours of fermentation culture to 40-42 hours of fermentation culture; and feeding vitamins into the fermentation liquid in a period from 20-24 hours of fermentation culture to 42-48 hours of fermentation culture, wherein the accelerant is one or more of ethylene diamine traacetic acid, ethylenediamine tetraacetic acid disodium and sodium triaminoacetate. The method provided by the invention can be used for improving the content of finishing point lysine, single pot acid supply amount and conversion ratio.

Description

technical field [0001] The invention relates to a production method of lysine. Background technique [0002] Lysine is one of the eight essential amino acids for human and animal nutrition. It plays an important role in regulating the metabolic balance in the body, improving the absorption of cereal protein in the body, improving human dietary nutrition and animal nutrition, and the growth and development of vinegar essence. At present, it is mainly used in medicine, food and feed industry. From the perspective of consumption structure, the consumption of lysine in feed accounts for nearly 90%, while the consumption of food and pharmaceutical intermediates only accounts for 10%. [0003] Generally, lysine is produced by inoculating lysine-fermenting strains into fermentation medium after seed culture to produce lysine. During the fermentation process of lysine, carbohydrates, proteins and precursors are needed to provide energy and form specific products. The nutrients gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12R1/15C12R1/19C12R1/13
Inventor 满云陈影荣玉凤李长辉
Owner COFCO BIOTECHNOLOGY CO LTD
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