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Method for fermenting L-lysine

A technology of lysine fermentation and fermentation method, applied in the field of fermentation production of L-lysine, can solve the problems of low end-point lysine content, total acid content and sugar-acid conversion rate, high production cost, etc. The effect of promoting bacterial growth and lysine synthesis, increasing end-point lysine content and shortening fermentation cycle

Inactive Publication Date: 2017-05-31
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, the end-point lysine content, total acid content and sugar-acid conversion rate obtained by the existing lysine preparation method are low, and the production cost remains high.

Method used

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  • Method for fermenting L-lysine

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Effect test

Embodiment 1

[0040] The fermentation method of L-lysine in this embodiment includes three steps of lysine primary seed cultivation, lysine secondary seed cultivation and lysine fermentation cultivation. The specific operation of each step is as follows:

[0041] 1. Primary seed culture of lysine

[0042] Prepare lysine primary seed medium. Lysine primary seed culture medium comprises sucrose 20g / L in the present embodiment, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 1.5g / L, ammonium sulfate 9g / L, yeast extract powder 5g / L, threonine 0.5g / L, methionine 0.5g / L, monosodium glutamate 7g / L, sodium pyruvate 0.5g / L, calcium acetate 1g / L.

[0043] With the prepared lysine first-grade seed culture medium, adjust the pH value to 6.0 with 20% (volume ratio) potassium hydroxide solution, pass steam to heat up the culture medium to 121 ° C, sterilize for 20 minutes, and turn on the circulating water to cool down to 36°C and kept constant. Start stirring at 300rpm, introduce sterile ai...

Embodiment 2

[0053] The fermentation method of L-lysine in this embodiment includes three steps of lysine primary seed cultivation, lysine secondary seed cultivation and lysine fermentation cultivation. The specific operation of each step is as follows:

[0054] 1. Primary seed culture of lysine

[0055] Prepare lysine primary seed medium. Lysine primary seed culture medium comprises sucrose 40g / L in the present embodiment, magnesium sulfate 1.5g / L, potassium dihydrogen phosphate 1.5g / L, ammonium sulfate 10g / L, yeast extract powder 8g / L, threonine 0.4g / L, methionine 0.4g / L, monosodium glutamate 9g / L, sodium pyruvate 0.6g / L, calcium acetate 3g / L. Adjust the prepared primary lysine seed medium to pH 6.0 with 20% potassium hydroxide, heat the medium to 121°C with steam, sterilize it for 20 minutes, and cool it down to 37°C with circulating water and keep it constant. Start stirring at 350rpm, introduce sterile air according to the ventilation ratio of 1:0.5, adjust the tank pressure to 0.0...

Embodiment 3

[0064] The fermentation method of L-lysine in this embodiment includes three steps of lysine primary seed cultivation, lysine secondary seed cultivation and lysine fermentation cultivation. The specific operation of each step is as follows:

[0065] 1. Primary seed culture of lysine

[0066] Prepare lysine primary seed medium. In the present embodiment, the lysine primary seed culture medium comprises 35g / L of sucrose, 0.7g / L of magnesium sulfate, 0.7g / L of potassium dihydrogen phosphate, 11g / L of ammonium sulfate, 7g / L of yeast extract powder, threonine 0.6g / L, methionine 0.46g / L, monosodium glutamate 7g / L, sodium pyruvate 0.5g / L, sodium acetate 1.5g / L.

[0067] Adjust the prepared primary lysine seed medium to pH 6.3 with 20% potassium hydroxide, heat the medium to 121°C by steaming it, sterilize it for 20 minutes, and cool it down to 37°C with circulating water and keep it constant. Start stirring at 300rpm, introduce sterile air according to the ventilation ratio of 1:0...

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Abstract

The invention relates to the field of fermentation, and particularly provides a method for fermenting L-lysine. Acetate is added into lysine primary seed culture media, lysine secondary seed culture media and lysine fermentation culture media, and formulas and fermentation processes for the various culture media are optimized. The method has the advantages that growth of lysine producing thalli and synthesis of lysine can be promoted, accordingly, the endpoint lysine content, the total acid content and the saccharic acid conversion rate can be obviously increased, and the fermentation periods can be obviously shortened.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting and producing L-lysine. Background technique [0002] L-lysine is a basic essential amino acid, and it is also the most important of the eight essential amino acids that cannot be synthesized by humans and animals, so it is often called the first limiting amino acid. The human body must obtain lysine through daily diet and supplements, but because the content of lysine in food is very low, and it is easily destroyed during processing, resulting in lysine deficiency. [0003] L-Lysine is widely used in pharmaceutical industry, food industry, animal feed and other fields. Adding a certain amount of L-lysine to cereal-based foods can increase the absorption rate and nutritional value of its protein; adding L-lysine to feed can improve the utilization rate of feed; in the pharmaceutical industry, L -Lysine is generally used as an amino acid ...

Claims

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Application Information

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IPC IPC(8): C12P13/08
CPCC12P13/08
Inventor 陈思弘穆晓玲许鹏李维理杨金环马德金杨为华张雪锋
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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