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Strain HD385 producing L-erythrulose through microorganism fermentation, and method

A technology for microbial fermentation and erythrulose, applied in the field of producing erythritol

Active Publication Date: 2014-07-30
深圳市博大生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few domestic and foreign reports on the microbial resources and microbial production of L-erythrulose, and the conversion rate of many strains is low. For example, when using Gluconobacter oxydans ATCC621 to produce L-erythrulose The conversion rate of the substrate is only 45~50%

Method used

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  • Strain HD385 producing L-erythrulose through microorganism fermentation, and method
  • Strain HD385 producing L-erythrulose through microorganism fermentation, and method
  • Strain HD385 producing L-erythrulose through microorganism fermentation, and method

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Experimental program
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Effect test

Embodiment 1

[0042] Utilize described bacterial strain HD385 to ferment the method for erythritol to produce L-erythrulose, be specifically: bacterial strain HD385 is inoculated in first-level seed culture medium (the first-level seed culture medium of described first-level seed culture medium) by the inoculum size of 10% earlier The composition is: erythritol 30 g / L, yeast powder 15 g / L, KH 2 PO 4 0.3 g / L, the balance is water, and the pH is natural), and cultured aerobically at 28°C for 24 h; then the cultured primary seeds were inserted into the secondary seed medium at an inoculation amount of 5% (the secondary The composition of the seed medium is: erythritol 30 g / L, yeast powder 15 g / L, CaCO 3 0.3 g / L, the balance was water, pH 6.0), and after 8 h, the cultivated secondary seeds were respectively inserted into the liquid fermentation medium containing different erythritol concentrations (250 mL triangular bottle, with a liquid capacity of 50 mL). Rotate at 200 rpm, ferment at 28...

Embodiment 2

[0047] The method for producing L-erythrulose by using the bacterial strain HD385 to ferment erythritol is specifically: the bacterial strain HD385 is directly inserted into the liquid fermentation medium according to the inoculum size of 4% (the liquid fermentation medium consists of: food Grade erythritol 100g / L, peptone 8g / L, KH 2 PO 4 3 g / L calcium carbonate, 3 g / L calcium carbonate, water as the balance, medium pH 7), fermented and cultured at 30°C under aerobic conditions for 72 h; the measured L-erythrulose content in the fermentation broth was 79.4 g / L.

Embodiment 3

[0049] The method for producing L-erythrulose by fermenting erythritol using the bacterial strain HD385 is specifically: directly inserting the bacterial strain HD385 into a liquid fermentation medium according to an 8% inoculum size (the liquid fermentation medium consists of: pharmaceutical Grade erythritol 100 g / L, corn steep liquor 20 g / L, KH 2 PO 4 5 g / L calcium carbonate, 8 g / L calcium carbonate, water as the balance, medium pH 5.5), fermented at 30°C for 72 h under aerobic conditions; the measured L-erythrulose content in the fermentation broth was 81.0 g / L.

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Abstract

The present invention relates to a strain HD385 producing L-erythrulose through microorganism fermentation, wherein the classification belongs to Acetobacteraceae (Gluconobacter) Gluconobacterkondonii, and the preservation number of the strain in the China General Microbiological Culture Collection Center is CGMCC No.8391. The present invention further provides a method for producing L-erythrulose by using the strain to ferment erythritol, wherein L-erythrulose can be significantly accumulated under an aerobic condition and can be up to 186.5 g / L, and the industrial production can be achieved.

Description

technical field [0001] The invention belongs to the field of biomanufacturing, and in particular relates to a bacterial strain HD385 for producing L-erythrulose by fermenting erythritol with aerobic microorganisms and a method for producing erythritol by fermenting the bacterial strain. Background technique [0002] L-erythrulose is a natural form of ketone sugar, the ketose form of erythrose. Its molecular formula is C 4 h 8 o 4， Molecular weight is 120.10, English name: L-erythrulose, is a yellow high viscous liquid with sweet taste. [0003] L-erythrulose can react with keratin amino acids in the outer or dead surface of the skin to make the skin brown and act as a natural self-tanning agent. At the same time, it is also a precursor compound of various anti-infective drugs. Its main use is in the cosmetics industry, generally as a self-tanning agent. Compared with dihydroxyacetone, its self-tanning effect is more durable and natural; if it is used together with dihyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/02C12R1/01
Inventor 朱龙华刘宇鹏李柱坚潘龙靳魁奇朱晓宏
Owner 深圳市博大生物技术有限公司
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