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Method for removing hydrogen sulfide by utilizing heterotrophic microorganisms

A technology for microorganisms and hydrogen sulfide, applied in the directions of methods using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of slow growth, unfavorable hydrogen sulfide absorption and oxidation, and difficulty in forming large biomass, etc. Achieve the effect of rapid growth, convenient cultivation and efficient removal

Active Publication Date: 2017-04-26
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such autotrophic bacteria grow slowly compared with heterotrophic bacteria, and it is difficult to form a large biomass, and their growth usually requires an acidic environment, but acidic conditions are not conducive to the absorption and oxidation of hydrogen sulfide
After searching, the method of using heterotrophic microorganisms to remove hydrogen sulfide under aerobic conditions has not been reported yet.

Method used

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  • Method for removing hydrogen sulfide by utilizing heterotrophic microorganisms
  • Method for removing hydrogen sulfide by utilizing heterotrophic microorganisms
  • Method for removing hydrogen sulfide by utilizing heterotrophic microorganisms

Examples

Experimental program
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Effect test

preparation example Construction

[0035] Preparation of polysulfides: 13 mg of sulfur powder and 70 mg of sodium sulfide were added to distilled water pre-deoxygenated with argon, sealed, and the pH value was adjusted to 9.3 with hydrochloric acid.

[0036] Preparation of acetone sulfur: Add excess sulfur powder into acetone, shake to dissolve fully, and the saturated solution contains about 20mM sulfur.

[0037] The detection method of sulfur disulfide is: add 0.55ml 1% boric acid to 1.5ml EP tube, heat in boiling water for 1min, add 0.25ml sample, add 0.2ml 0.1M potassium cyanide and heat for 1min, cool at room temperature, add 0.1ml Ferric nitrate reagent, mix immediately, if there are cells, remove by centrifugation, measure OD 460nm , and the blank control was deionized water.

[0038] Sulfite, sulfate and thiosulfate were detected by ion chromatography (ICS-1100system; Dionex), and the specific conditions were: mobile phase flow rate: 1ml / min, column temperature: 30°C, suppressor type: ASRS_4mm, suppres...

Embodiment 1

[0039] Example 1: Research on the hydrogen sulfide oxidation rate of five heterotrophic bacteria.

[0040] Five strains of heterotrophic bacteria (Gluconobacter oxydans 621H, Pseudomonas putida S16, Pseudomonas aeruginosa PAO1, Ralstonia (Cupriavidus necator) JMP134 , or Burkholderia cepacia (Burkholderia cepacia) ATCC25416) at 30 degrees Celsius and 200rpm shaking culture for more than 20 hours, the bacterial turbidity is greater than OD 600nm When =4, the cells were collected by centrifugation and washed once with pure water, and then the treated heterotrophic cells were dissolved in 4-(2- Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES:4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer, and adjust the bacterial turbidity to OD 600 =2. In application, the cultured bacteria solution can also be used to deal with sulfide directly or after dilution.

[0041] Add 10 mL of the adjusted heterotrophic bacterial cell suspension into a 50 mL centrifuge tube, and then ...

Embodiment 2

[0045] Example 2: Research on the induction effect of hydrogen sulfide.

[0046] Ralstonia (Cupriavidus pinatubonensis) JMP134, Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1, Burkholderia cepacia (Burkholderia cepacia) ATCC 2541, or Pseudomonas putida (Pseudomonas putida) S16 bacteria When the method in Example 1 was cultivated to the early logarithmic growth period (OD 600 =0.5), the final concentration of 20μM Na was additionally added in the medium 2 S, polysulfide or acetone sulfide, continue to cultivate and induce for 1 hour, then collect the thalline according to the method in Example 1, measure the oxidation rate of hydrogen sulfide, and compare with the control group without induction, the results show that hydrogen sulfide, polysulfide And acetone sulfur can induce bacteria to enhance their ability to oxidize hydrogen sulfide, see image 3 .

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Abstract

The invention discloses a method for removing hydrogen sulfide by utilizing heterotrophic microorganisms. Specific heterotrophic microorganisms are added in a device for removing hydrogen sulfide, an aerobic reaction is performed in a set reaction system, and removal on H2S, HS- or / and S2- in the reaction system of the device is implemented, wherein the heterotrophic microorganisms mean a category of heterotrophic bacteria of which a genome comprises a sulfide:quinone oxidoreductase gene and a persulfid dioxygenase gene and which can oxidize sulfides to generate zero-valent sulphur and thiosulfate, or sulfate. According to the method disclosed by the invention, the screened heterotrophic bacteria have efficient capacity of oxidize exogenous hydrogen sulfide, has the characteristics of high growth speed, low culture cost and capacity of forming great biomass, effectively solves the problem of condition limitation in the prior art in which autotrophic bacteria are used for remove hydrogen sulfide, and has a great application potential in the industrial production.

Description

technical field [0001] The invention relates to a method for biologically removing sulfide, in particular to a method for utilizing heterotrophic microorganisms to remove sulfide (H 2 S, HS - or / and S 2- )Methods. Background technique [0002] Hydrogen sulfide is a poisonous gas with a pungent smell, and is the main waste gas component widely existing in industrial production processes (such as sewage treatment plants, animal husbandry and composting plants, etc.). At present, the removal methods of harmful hydrogen sulfide gas are mainly divided into physical methods, chemical methods and biological methods. Compared with chemical methods and physical methods, biological methods have irreplaceable advantages in the removal of hydrogen sulfide, and have broad application prospects. [0003] In the process of biological removal of hydrogen sulfide, microorganisms play a vital role in the oxidation and absorption of hydrogen sulfide. At present, there are mainly domesticat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D53/84B01D53/52C12N1/20C12N1/36C12R1/40C12R1/385C12R1/01
CPCB01D53/52B01D53/84B01D2251/95C12N1/20C12N1/36Y02A50/20
Inventor 荀鲁盈侯宁可刘红蕾
Owner SHANDONG UNIV
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