34 results about "Androstenone" patented technology

Disclosed is a method and a product of a chicory root product for reducing taint in animals, said method comprising feeding to an animal a chicory root product during at least one day prior to slaughtering the animal. By feeding animals with the chicory root product this improves the quality of meat, prevents or reduces female and male animal taint, primarily boar taint caused by skatole and / or androstenone. The invention also relates to methods for improving the health status of animals e.g. by reducing infections by pathogens in the gastrointestinal tract and to methods for reducing animal caused odors in general. The chicory root product comprises inulin / fructan (fructo-oligosaccharides), other low molecular weight sugars and secondary metabolites.

Described is a method of conditioning an invertebrate to detect a compound at and beyond a threshold range. In one embodiment the conditioned invertebrate is Microplitis croceipes. Compounds the invertebrate can detect include skatole and androstenone. An additional step includes selecting a compound and negatively conditioning the invertebrate at a concentration wherein the invertebrate will not respond to negatively conditioned compound concentration.

A perfume composition comprising a perfume component capable of inhibiting the production of odoriferous steroids by micro-organisms on the skin. The perfume component is capable of inhibiting the biotransformation of androstadienols to androstenones.

The invention discloses a fermentation process method for biologically synthesizing 7alpha,15alpha-dihydroxy androstenone by taking colletotrichum lini ST-1 as a strain. The method is characterized in that a certain amount of substrate dehydroepiandrosterone is added at the beginning of a fermentation process, and the substrate is fed again after the substrate dehydroepiandrosterone is cultured for a period of time, so that not only a key enzyme can be pre-induced in the transformation process, but also the toxicity to the strain cell caused by feeding the substrate at a time can be reduced. Therefore, the yield of the product can be increased. Through the adoption of the fermentation process, the 7alpha,15alpha-dihydroxy androstenone is synthesized, meanwhile, the feeding concentration of the substrate can reach 8 g / L (weight of substrate / volume of fermentation broth), and the transformation rate can reach about 90%. Compared with a once feeding way, the way of feeding the substrate reduces the toxicity to the cell caused by the substrate, increases the yield of the product to a certain extent, and increases the feeding amount of the substrate.

The invention belongs to the biotechnology field, and relates to a method for synthesizing novel 7alpha-hydroxy-androstenone (7alpha-OH-AD) steroid by the efficient utilization of colletotrichum lini ST-1 hydroxylation androstane-4-alkene-3, 17-diketone (AD). The method has the advantages that the conversion rate reaches up to 90% by adopting a two-step conversion method under the condition that the feeding amount of a substrate is 10g / L, and the total yield of the 7alpha-OH-AD product is 79.32%.

A composition comprising Androstenone is described for the modification of undesirable or harmful stress-related behaviors or other behaviors or physiology in a variety of vertebrate species, as well as methods of using the compositions in vertebrates from a species different than the species in which the Androstenone is a naturally occurring pheromone.

The invention discloses phytosterol of microbial transformation, which is a strain which only produces androstene dione, pertaining to the fermentation technology field in biological engineering. The screened strains are classified and named as starch degrading bacillus (Bacillus a myloiquefaciens.) ST 06-95 and preserved in the China Center for Type Culture Collection with a preservation number of CCTCC No. M208135. Samples are collected from the nature separated for primary screening and put in slant preservation; wild strains ST 06 are taken as starting strains and screened by ultraviolet mutagenesis to obtain strains ST06-95 which mainly produce androstenone; and the growth of strains enters a logarithmic growth phase at the temperature of 30 DEG C, the pH value of 7.0 and a rotation speed of 220r / min and after culture lasting for 60h. Thallus in the growth phase are inoculated into a fermentation culture medium according to inoculation amount of 12 percent (V / V) and fermented for 6 days to 7 days at the temperature of 30 DEG C, the pH value of 7.0 and a rotation speed of 220r / min. When quantitative detection is carried out on the fermented products, the product content of ADD exceeds 1500mg / l, and ADD accounts for 98 percent of the total amount of AD and ADD. The microbial transformation provides a basis for the industrialization of the microbial transformation of steroidal medicaments.

The invention discloses a spray for sows and a preparation method thereof. The spray can replace boars to be applied to estrus induction and check of sows and before and after breeding so as to prevent cross contamination of pigs in farms and prevent epidemic diseases. The spray comprises raw materials in parts by weight as follows: 1-5 parts of androstene substances, 1-5 parts of essence, 1-5 parts of lanolin, 1-5 parts of decavitamin, 1000-2000 parts of alcohol solvents, 1-9 parts of a cosolvent, 1-3 parts of an antioxidant and 10000-20000 parts of deionized water. The product can induce estrus of sows and effectively stimulate sexual reactions of sows to promote ovulation. Meanwhile, in the epidemic situation period, the traditional mode of estrus induction by boars can be avoided, so that spreading of epidemic situation is blocked.

The invention relates to a pheromone composition, in particular to a pheromone composition with an identification function and a preparation thereof. The pheromone composition comprises a component A,a component B and a component C, wherein the component A is androstenonev and / or androstanol; the component B comprises one or more of geraniol, tiglic aldehyde, palmitic acid, oleic acid, linoleic acid, capric acid, myristic acid, lauric acid and 3-methylindole; the component C comprises: a coloring agent. According to the method, multiple pheromones are combined for use, synergistic componentsfrom plants are added, the estrus rate and the reproductive success rate of the sows are increased, and meanwhile, the coloring agent is added for identification, so that the estrus identification efficiency is improved. Moreover, in the preparation process of the pheromone composition preparation, the androstanol is protected from being oxidized by filling nitrogen, so that the stability of the preparation is guaranteed, and the use of components such as an antioxidant and a preservative is avoided, and the product is safer and meets the green production requirement.

The present invention discloses a novel pig feed preparing process which relates to the technical field of livestock feed and the formula of the pig feed consists of polypeptides, corn protein powder, transfer factor lyophilized powder, rheum palmatum, baicalin and probiotics. The preparation process of the pig feed is as follows: the production temperature is controlled below 50 DEG C; firstly the polypeptides and the corn protein powder are premixed, and transfer factor lyophilized powder, rheum palmatum, baicalin are added, mixed and stirred thoroughly; and if the temperature of the stirrer rises after stirring, the probiotics are added and fully mixed after the temperature drops. The novel pig feed preparing process is mainly used for growing muscle and gaining weight of livestock and poultry, decomposing carcass androstenone and skatole, improving meat quality and increasing body resistance to diseases, improving feed conversion rate, reducing nitrogen emissions, improving pig house environment and increasing lean meat rate.

The invention discloses a preparation method of exemestane, relates to the field of an anti-cancer drug, and particularly relates to exemestane. The preparation method of the exemestane includes stepsof preparing 6-methylene androstenone glycol acetone; under the nitrogen protection, orderly adding paraformaldehyde, dimethylamine hydrochloride and isoamyl alcohol in a four-opening reaction bottle; heating and backflowing water for 2 hours; adding androstenone glycol acetone, preserving temperature and stirring at 140 DEG C; performing high performance liquid chromatography tracking detectionreaction; finishing reaction for 15 hours, and cooling to room temperature; adding distilled water to wash; after layering, drying it by anhydrous magnesium sulfate, depressurizing and recycling solvent; performing column chromatography on silica gel to obtain white solid; secondly, preparing exemestane; orderly adding 6-methylene androstenone glycol acetone, p-toluenesulfonic acid and tetrahydrofuran in a four-opening bottle under the nitrogen protection. The preparation method is simple in technical route, easy to obtain raw materials, low in cost, high in yield, and overcomes the defects existed in the prior art; therefore, the preparation method is applicable to industrial production.

This invention relates to methods of formulating steroid pheromones as novel stable emulsions. These emulsions can be used for administration of the steroid pheromones to living organisms such as humans or pigs in place of current organic solvent or pressurized aerosol formulations that present hazards in both shipping and application. Uses relating to pigs are in stimulation of sexual maturation, diagnosis of the onset and timing of oestrus in female pigs, and inducing boars to accept dummy sows. The water-based emulsions can include 5 -androst-16-en-3-one and 3 -hydroxy-5a-androst-16-ene for administration to pigs. The use of water-based emulsions eliminates the need for formulation of the steroid pheromones in organic solvents, delivery of these pheromones as pressurized aerosols, and following the precautions required to reduce hazards during shipping and handling. The water-based emulsions allow for treatment of living organisms where a liquid organic solvent formulation or aerosol of the pheromones might be harmful to the organism or a substrate.

The invention relates to a porcine transcription factor coup-tf1, its recombinant vector and application. The nucleotide sequence of the porcine transcription factor coup-tf1 is shown as SEQ ID NO.19. Overexpression of the porcine transcription factor coup-tf1 can reduce the expression of CYP11A1, CYP17A1 and StAR genes, lower the androstenone content in pork, inhibit the accumulation of odor substance, and ultimately improve the quality of the pork.

The invention belongs to the field of pharmaceutical chemistry synthesis, and relates to a preparation method for a key intermediate 2alpha, 3alpha-epoxy-16beta-(1-pyrrolidyl)-5alpha- androstane-17 hydroxy of rocuronium bromide of steroid muscle relaxant, which includes the steps: leading inexpensive and available 5alpha-androstane-2-alkene-17 ketone and isopropenyl acetate to undergo ester exchange to generate cresyl violet acetate, oxidizing the cresyl violet acetate by the meta-chloroperoxybenzoic acid to obtain an epoxy compound, carrying out an open-loop substitution reaction between the meta-chloroperoxybenzoic acid and pyrrolidine under the alkaline condition, and reducing via sodium borohydride so that the rocuronium bromide key intermediate 2alpha, 3alpha-epoxy-16beta-(1-pyrrolidyl)-5alpha-androstane-17 hydroxy is prepared. An ester group with large steric hindrance is generated at the 17th position when the 5alpha-androstane-2-alkene-17 ketone and the isopropenyl acetate undergo ester exchange in the process to lead stereoscopic steric hindrance of a whole steroid ring to be large, so that attack on the lower portion of the steroid ring can be performed when epoxidation is carried out, and further stereoselectivity is high when an epoxide is formed, and then a beta configuration can be directly formed when the pyrrolidine is used for substitution. The synthesis method is good in stereoselectivity.

The invention relates to a pheromone composition, in particular to a pheromone composition spray preparation and a preparation method and application thereof. The pheromone composition spray preparation is prepared from the following components in percentage by mass: 0.001-0.02% of androstanone and / or androstanol, 0.001-0.02% of a herba epimedii extract, 0.2-20% of dimethyl sulfoxide, 0.01-0.5% oftween-80, 0.1-1.0% of a preservative, and 80.0-99.0% of purified water. The spray preparation is safer and more reliable, the reproductive performance (namely, the higher crossable estrus rate) of sows is reliably improved, the utilization rate of the sows is increased, and the fertility of the sows is improved.

The invention discloses a fermentation process method for biologically synthesizing 7alpha,15alpha-dihydroxy androstenone by taking colletotrichum lini ST-1 as a strain. The method is characterized in that hydrogen peroxide of certain concentration is flowingly added in the fermentation process to improve oxygen dissolving in the fermentation process and increase the yield of the product. By the fermentation process, the product 7alpha,15alpha-dihydroxy androstenone is synthesized, meanwhile, the feeding concentration of substrate can reach 8 g / L (weight of substrate / volume of fermentation broth), and the transformation rate can reach about 90%. By the method, the oxygen dissolving problem in the fermentation process can be solved better, and the expression of a key enzyme in the fermentation process is induced to a certain extent, so that the transformation efficiency is improved and the yield of the product is increased.

The invention discloses a preparation method of 17 beta-androst-4-ene-3-one-17-carboxylic acid. The preparation method comprises that 4-androstenedione (called as 4AD for short) as a raw material andtriethyl orthoformate undergo a reaction in an organic solvent under acid catalysis, the reaction product is after-treated to form an etherate, the etherate is dissolved in an organic solvent and undergoes a reaction with trimethylsulfonium iodide under strong base catalysis, the product is after-treated to form an epoxide, the epoxide is dissolved in an organic solvent and then is subjected to rearrangement under acid catalysis to form an aldehyde, and aldehyde is dissolved in an organic solvent and undergoes a catalytic oxidation reaction with hydrogen peroxide acid to produce 17 beta-androst-4-ene-3-one-17-carboxylic acid. The 17 beta-androst-4-ene-3-one-17-carboxylic acid has a melting point of 244-246 DEG C, HPLC content of 99.0% or more and a reaction weight total yield of 70-72%. Compared with the traditional method, the preparation method utilizes cheap and easily available raw materials, has simple and environmental friendly processes and a high synthesis yield, produces highquality products, reduces a cost by 30-40% and is conducive to industrial production.

The invention relates to a porcine transcription factor coup-tf1, its recombinant vector and application. The nucleotide sequence of the porcine transcription factor coup-tf1 is shown as SEQ ID NO.19. Overexpression of the porcine transcription factor coup-tf1 can reduce the expression of CYP11A1, CYP17A1 and StAR genes, lower the androstenone content in pork, inhibit the accumulation of odor substance, and ultimately improve the quality of the pork.

The invention relates to the field of medicine preparation, in particular to a synthetic method of androstenone. The preparation method comprises the following steps: S100, catalyzing testosterone by a catalyst, and carrying out hydrogenation addition reaction to obtain a compound I; S200, converting a 17-site hydroxyl group of the compound I into a halogen group or a sulfonate group which is easy to leave, so as to obtain a compound II; and S300, subjecting the compound II and alkali to an elimination reaction under the heating condition, namely, a dehalogenation reaction or a desulphonate reaction, so as to obtain androstenone. The synthesis method of androsteneone has the following beneficial effects: the raw materials are cheap and easy to obtain; the process is simple, the route is short, and the requirement on equipment is low; dangerous reagents and operation are avoided, and large-scale industrial production is easy to realize; and isomer impurities are not generated in the synthesis process, and the yield of the target product androsteneone is high.

The invention discloses a method for preparationof 3beta-acetoxyandrostane-5-ene-17-one by utilizing androstane-3-beta hydroxyl-17-one-6alpha-boric acid separated from androstane-5-ene-17-one chemicalreduction reaction solid waste through the four steps of reactions of esterification, oxidation, sulfonic acid esterification and elimination. According to the method, the steroid solid waste can beeffectively recycled, the synthesis method is efficient, the reaction conditions are mild, and the cost of related products is correspondingly reduced while the environmental pollution is reduced.

The invention belongs to the technical field of steroid drug intermediates. The invention provides a preparation method of androst-2-ene-17-one, which comprises the following steps: 1) mixing epiandrosterone, a halogen-containing compound, a catalyst and a solvent, and performing halogenation reaction to obtain a compound 1; and 2) mixing the compound 1, a catalyst and a solvent, and performing elimination reaction to obtain androst-2-ene-17-one. By reasonably selecting the catalyst and controlling the raw material proportion and process conditions, the yield and purity of the target product are remarkably improved; and moreover, the reaction conditions are mild, the operation is simple, the production cost is low, and the reaction selectivity is good. The yield of the androst-2-ene-17-one prepared by the preparation method is greater than or equal to 94%, and the purity is greater than or equal to 98.5%.

Genetic markers are disclosed with a useful association with boar taint that can be used for screening and selection of pigs for those with more favorable boar taint characteristics associated with androstenone / skatole metabolism. Specific polymorphic alleles of the 3αHSD, 3βHSD, CYP17A1, CYP2A, CYP2E1, CYTB5, BAC-CT and / or SULT1A1 genes are disclosed for tests to screen pigs to determine those more likely to produce desired boar taint traits.