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A kind of analysis method of dehydroepiandrosterone and its α-isomer

A technology of dehydroepiandrosterone and an analysis method, which is applied in the analysis field of dehydroepiandrosterone and its α-isomer, can solve problems such as poor separation effect, and achieves easy control, good impurity separation effect, and detection cost. low effect

Active Publication Date: 2022-01-28
湖北竹溪人福药业有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problem of poor separation effect of existing analysis techniques, the present invention provides a brand-new analysis method for dehydroepiandrosterone and its α-isomer

Method used

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  • A kind of analysis method of dehydroepiandrosterone and its α-isomer
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  • A kind of analysis method of dehydroepiandrosterone and its α-isomer

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Effect test

Embodiment 1

[0030] Chromatographic conditions:

[0031] Chromatographic column: phenyl column, Suplex PKB-100, 250*4.6mm, 5µm;

[0032] Mobile phase: ethanol: acetonitrile: water = 15:15:70 (V / V / V)

[0033] Injection volume: 20μl

[0034] Detection wavelength: 210nm

[0035] Flow rate: 1.0ml / min

[0036] Operation: Column temperature: 30°C; take an appropriate amount of test sample, accurately weigh it, dissolve it with acetonitrile and dilute it to a 2.0 mg / ml solution, shake well, accurately measure 20 μl and inject it into the liquid chromatograph, record the chromatogram to the retention time of the main peak 1.2 times.

Embodiment 2

[0038] Chromatographic conditions:

[0039] Chromatographic column: phenyl column, Suplex PKB-100 250*4.6mm, 5µm;

[0040] Mobile phase: ethanol: acetonitrile: water = 20: 20: 60 (V / V / V)

[0041] Injection volume: 20μl

[0042] Detection wavelength: 200nm

[0043] Flow rate: 1.2ml / min

[0044] Operation: Column temperature: 35°C; take an appropriate amount of test sample, accurately weigh it, dissolve it with acetonitrile and dilute it into a 2.5mg / ml solution, shake well, accurately measure 20μl and inject it into the liquid chromatograph, record the chromatogram to the retention time of the main peak 1.5 times.

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Abstract

The invention discloses an analysis method for dehydroepiandrosterone (3β-hydroxyandrost-5-en-17-one), which is analyzed by HPLC, specifically using a phenyl column, with ethanol, acetonitrile and water as the flow Phase, to achieve accurate detection of dehydroepiandrosterone. The analysis method of the invention is easy to operate and improves the separation efficiency and detection accuracy.

Description

technical field [0001] The invention relates to the technical field of steroid drug analysis, in particular to an analysis method for dehydroepiandrosterone and its α-isomer. Background technique [0002] Dehydroepiandrosterone, English name: Dehydroepiandrosterone, chemical name: 3β-hydroxy-5-androsten-17-one, molecular formula C 19 h 28 o 2 . DHEA is an important intermediate and raw material for the manufacture of steroidal drugs. It also has the functions of delaying aging, maintaining youthful vitality, enhancing physical fitness, improving mood and sleep, improving memory, regulating immune system functions, and improving body immunity. . [0003] In the process of realizing the present invention, the inventors found at least the following problems in the prior art: In recent years, more and more manufacturers use phytosterol fermentation product androstenedione (4-AD) as raw material through esterification, condensation Ketone, reduction, hydrolysis to synthesiz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
CPCG01N30/02G01N30/06G01N30/34G01N2030/027
Inventor 侯海波杨艳青郑建雄张阳洋汪洋赵静刘林李廿罗宇肖康狄飞飞蔡啸
Owner 湖北竹溪人福药业有限责任公司
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