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Porcine transcription factor coup-tf1, its recombinant vector and application

A technology of coup-tf1 and transcription factors, which is applied in the fields of application, recombinant DNA technology, and the use of vectors to introduce foreign genetic materials, etc., can solve problems such as difficult consumer acceptance, achieve weakened inhibitory effects, good application prospects, and reduce pork smell Effect

Active Publication Date: 2014-02-05
ARMY MEDICAL UNIV
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Problems solved by technology

Compared with castrated boars, the comprehensive production efficiency of whole boars, that is, non-castrated boars, will increase by 30%, which has significant social and economic value, but the existence of boar smell makes it difficult for consumers to accept. Elimination or Reducing boar taint is a problem that must be solved to promote whole boar breeding

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  • Porcine transcription factor coup-tf1, its recombinant vector and application
  • Porcine transcription factor coup-tf1, its recombinant vector and application
  • Porcine transcription factor coup-tf1, its recombinant vector and application

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Embodiment Construction

[0032] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to examples. The experimental methods for which specific conditions are not indicated in the preferred embodiments are usually in accordance with conventional conditions or in accordance with the conditions suggested by the manufacturer.

[0033] The experimental materials used in the present invention are all commercially available products unless otherwise specified.

[0034] At present, the research on improving boar taint is mainly to carry out marker-assisted selection research on the fat deposition level of androstenone and skatole, one is quantitative trait locus (quantitative trait locus, QTL) mapping research; the other is candidate gene research . Androstenone and skatole are compounds that cause pork taint, and their deposition in adipose tissue depends on their synthesis and degradation metabolism levels in pigs. Studies have found that CYP11A1, CYB5A, CY...

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Abstract

The invention relates to a porcine transcription factor coup-tf1, its recombinant vector and application. The nucleotide sequence of the porcine transcription factor coup-tf1 is shown as SEQ ID NO.19. Overexpression of the porcine transcription factor coup-tf1 can reduce the expression of CYP11A1, CYP17A1 and StAR genes, lower the androstenone content in pork, inhibit the accumulation of odor substance, and ultimately improve the quality of the pork.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and particularly relates to a porcine transcription factor coup-tf1, and also relates to a recombinant vector containing the porcine transcription factor coup-tf1 and its application. Background technique [0002] Pig farming is the main industry of animal husbandry in my country, and pork is the main animal food for Chinese people. Compared with castrated boars, the comprehensive production efficiency of whole boars, that is, non-castrated boars, will increase by 30%, which has significant social and economic value, but the existence of boar smell makes it difficult for consumers to accept. Elimination or Reducing boar taint is a problem that must be solved to promote whole boar breeding. Studies have shown that boar taint mainly comes from two substances deposited in pork adipose tissue: skatole and androstenone (5α-ster-16-en-3α-one), of which androstenone is a substance in the interstitial...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/47C12N15/85A01K67/027
Inventor 刘宇魏泓商海涛郭科男郑小波吴群
Owner ARMY MEDICAL UNIV
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