Penicillium capable of giving high yield of alpha-alactosidase as well as its application for preparing feeding alpha-alactosidase in solid state fermentation

A technology of galactosidase and Penicillium yankii, applied in the application, enzyme, enzyme and other directions, can solve the problems of high price, low output, complex production process, etc., and achieve the effect of low production cost and improved production performance

Inactive Publication Date: 2006-10-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although α-galactosidase is widely distributed, microorganisms, animals and plants can produce α-galactosidase, but due to the complex production process, the output is small, and the price is relatively expensive

Method used

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  • Penicillium capable of giving high yield of alpha-alactosidase as well as its application for preparing feeding alpha-alactosidase in solid state fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Effect of α-galactosidase on growth performance of piglets

[0058] With the continuous development and improvement of bioengineering technology, animal husbandry production has also undergone tremendous changes; animal nutrition and feed processing are not limited to the development of new feed raw materials and research on the nutritional requirements of livestock and poultry. Enzyme preparations to improve feed utilization and reduce the adverse effects of anti-nutritional factors in feed have attracted people's attention; environmental protection is a hot issue that is generally concerned in the world today. a feasible path;

[0059] Because soybeans contain raffinose and stachyose, which cause flatulence, the main cause of flatulence is the lack of enzymes in the intestine that can hydrolyze them - α-galactosidase; the small intestine cannot utilize α-galactosides; after they enter the large intestine , is fermented and utilized by the microbial flora o...

Embodiment 2

[0091]A total of 360 healthy 1-day-old AA broiler roosters with similar birth weight were selected and randomly divided into three groups: the group fed corn and soybean meal without enzyme, the group with 0.01% enzyme, and the group with 0.05% enzyme. Each group was treated with 6 replicates. Repeat with 20 chickens for a period of 42 days. The results show that adding α-galactosidase can make the daily gain of broiler body weight significantly different (p<0.05), and reduce the feed-to-meat ratio difference significantly (p<0.05), but there is no effect on feed intake; therefore, Adding α-galactosidase to the diet can improve the production performance of broiler chickens

[0092] Improving the utilization efficiency of livestock and poultry feed has always been a research area of ​​great concern in animal nutrition, especially how to improve the utilization value of feed is more worthy of attention under the current shortage of feed raw materials; an important factor hinder...

Embodiment 3

[0118] Embodiment 3: the influence of α-galactosidase on broiler growth performance:

[0119] This embodiment selects 360 healthy, 1-day-old AA broiler cocks with similar birth weights, and is randomly divided into 3 treatments (corn soybean meal type without enzyme group, enzyme 0.01% group, enzyme 0.05% group, each processing 6 chickens) Repeat, every repeat 20 chickens, by a period of 42 days.The results show that adding α-galactosidase can increase the daily weight gain of broilers significantly (p<0.05), and reduce the feed-to-meat ratio difference significantly (p<0.05) , but has no effect on feed intake; therefore, the application of α-galactosidase in the diet can improve the performance of broiler chickens;

[0120] Improving the utilization efficiency of livestock and poultry feed has always been a research area of ​​great concern in animal nutrition, especially how to improve the utilization value of feed is more worthy of attention in the current situation of relat...

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Abstract

A Yangqi blue mold (CGMCC No.0668) able to generate alpha-galaotosidase and its application in preparing feed-class alpha-galactosidase by solid fermentation are disclosed. It features biologically that its colony can properly grow on malt wort plate for 7 days.

Description

field of invention [0001] The invention belongs to the field of biotechnology, and in particular relates to a Penicillium yanchii strain capable of high-production alpha-galactosidase and its application in solid-state fermentation for preparing alpha-galactosidase for feed. Background technique [0002] Improving the utilization efficiency of livestock and poultry feed has always been a research area of ​​great concern in animal nutrition, especially how to improve the utilization value of feed is more worthy of attention in the current situation of relatively scarce feed raw materials. An important factor that hinders the full utilization of feed by livestock and poultry is the various anti-nutritional factors contained in feed ingredients. These anti-nutritional factors interfere with the normal digestive and metabolic functions of livestock and poultry, thereby affecting the full play of animal production potential and reducing feed utilization efficiency. There is a hi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/40A23K1/165C12R1/80
Inventor 李德发潘宝海陆文清谯仕彦朴香淑张丽英邢建军胥学新
Owner CHINA AGRI UNIV
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