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Biocontrol strain KMXU28 for controlling paris rhizome root rot and biocontrol agent thereof

A technology of biocontrol strains and biocontrol agents, applied in biocides, fungicides, biocides, etc., to protect farmland ecosystems, increase microbial activity, and inhibit growth

Inactive Publication Date: 2013-04-24
KUNMING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to prevent and control the root rot of Dianzhong in the current agricultural production, which is difficult and costly in cultivation, and it is not suitable to use chemical pesticides and pesticide residues. Problem, provide a kind of biocontrol bacterial strain KMXU28 and biocontrol bacterial agent thereof for the prevention and treatment of the root rot of Diana polyphylla

Method used

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  • Biocontrol strain KMXU28 for controlling paris rhizome root rot and biocontrol agent thereof
  • Biocontrol strain KMXU28 for controlling paris rhizome root rot and biocontrol agent thereof
  • Biocontrol strain KMXU28 for controlling paris rhizome root rot and biocontrol agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of biocontrol agent

[0028] a. Transplant the strain KMXU28 into a test tube for one-ring activation, and incubate at 28°C for 48 hours; the PSA liquid medium in the test tube is: Weigh 200g of potatoes, wash, peel and chop them, add 1000ml of water to boil for half an hour, filter with gauze , add 20g of sucrose and 17g of agar, fully dissolve, filter with gauze while hot, and sterilize with 15 pounds of steam (121°C) for 20 minutes;

[0029] b. Take one ring of activated bacterial lawn and transfer it into 600ml 1000ml Erlenmeyer flask with formula: tryptone 10g / L, yeast extract 5g / L, sodium chloride 5g / L, pH7.0 LB culture medium, Cultivate at 120 rpm and 28°C for 72 hours to obtain fermentation broth;

[0030] c. Inoculate the fermented bacteria liquid in the seed tank at the ratio of inoculum 1:200, ferment and culture in the same LB culture liquid as step b, the temperature of the seed tank is 29°C or 30°C or 31°C, ferment for 16 or ...

Embodiment 2

[0033] Embodiment 2: plate antagonism test

[0034] According to Fang Zhongda's "Plant Disease Research Methods", the plate-supported culture test method was used to evaluate the inhibitory effect of KMXU28 bacteria on the root rot of P. The results are as follows, the growth inhibition rate of KMXU28 to P. dianii root rot was 93.00% (Table 1). It shows that the KMXU28 strain has a significant inhibitory effect on the root rot of P. yunnanensis.

[0035] Table 1 Inhibitory effect of KMXU28 on the root rot pathogens of Prunus yunnanensis and related pathogens

[0036]

Embodiment 3

[0037] Example 3: Greenhouse Control Efficiency Test of Prunus edulis Root Rot

[0038]Three treatments were set up, treatment 1 was the sterile water control group, treatment 2 was the KMXU28 bacterial agent dilution, and treatment 3 was the fungicide carbendazim (Nantong Runhong Biochemical Co., Ltd.). Transplant the annual tissue-cultured seedlings of Parisia diatriana into the diseased soil of P. KMXU28 bacterial agent 1: 50 times the bacterial solution for root irrigation, 30-35 crops per kilogram of liquid irrigation; 100 days after the crops are transplanted, use KMXU28 bacterial agent 1: 50 times the bacterial solution for root irrigation, 30-35 plants per kilogram of liquid irrigation crop. The roots were irrigated every two months during the whole growth period, and the other water control and carbendazim pesticide treatments were the same as the above methods and dosages. The amount of the preparation applied per 667㎡ is determined according to the number of trans...

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Abstract

A biocontrol strain KMXU28 for controlling paris rhizome root rot and a biocontrol agent thereof belong to the technical field of bio-pesticides. The accession number of strain KMXU28 is CGMCC NO. 6594. The biocontrol agent is obtained by the following steps: transplanting strain KMXU28 into a tube for activation by using one-inoculating loop of the strain to culture at 28 DEG C for 48 hours; transplanting activated bacterial lawn into 600ml LB culture medium contained in a 1000ml conical flask to culture under the conditions of 120 r / min, 28 DEG C for 72 hours to obtain a fermentation broth; incubating the fermentation broth in LB medium in a seed tank for fermentation culture at a ratio of 1:200, the temperature of the seed tank being 30-32 DEG C, and obtaining strain used for fermentation after fermentation for 16-18 hours; incubating the strain used for fermentation in a fermentation tank at a ratio of 1:20 for fermentation culture at a temperature of 30-32 DEG C for 72-96 hours, and saving the obtained material as a biocontrol bacteria solution; and centrifuging the biocontrol bacteria solution at 5000rpm for 6 min, to prepare an active bacterial agent with a preservation solution at 1:50 (g / ml). The biocontrol agent of the invention is low in cost, non-toxic and free of pollution, and can effectively prevent the successive cropping obstacle of soil-borne diseases of paris rhizome, thus improving harvest of paris rhizome.

Description

Technical field: [0001] The present invention involves KMXU28 and its biochemical agents, which are involved in the prevention and treatment of roots of heavy buildings, and their biological pesticide technology. Background technique: [0002] The Yunnan Chonglou is the dried root stems of the lily plant plant Yunnan Chonglou Paris Polyphylla Smith Var. Yunnannsis (Franch.) Hara. It is one of the varieties collected in the 2010 version of "Chinese Pharmacopoeia" [1].Tumor, hemostasis, analgesic, sedative, immune regulation and other biological activity are Chinese medicinal materials with significant effects in traditional medicine, which are widely used [2].In recent years, with the continuous development of the medicinal effect of heavy buildings, the demand for the demand for drugs and corporate drugs has risen sharply. The price of heavy buildings has continued to rise.The market needs, and the contradiction between supply and demand is also increasingly prominent. For a lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00A01P21/00C12R1/125
Inventor 余磊赵建荣
Owner KUNMING UNIV
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