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A strain with antagonistic effect on Sclerotinia sclerotiorum of Chinese cabbage and its culture method and application

A technology of sclerotinia and antagonism, applied in the field of strains and their cultivation, can solve the problems of increasing pesticide residues, soil, water and ecological environment pollution, and achieve safe and effective growth, no environmental pollution, and protection of farmland ecosystems. Effect

Inactive Publication Date: 2020-07-31
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

The excessive use of chemical pesticides in traditional agriculture will not only increase the amount of pesticide residues, but also lead to the resistance of many crop diseases and insect pests, and cause pollution to soil, water and ecological environment

Method used

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  • A strain with antagonistic effect on Sclerotinia sclerotiorum of Chinese cabbage and its culture method and application

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Embodiment Construction

[0010] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0011] Effect of Antagonistic Antibacterial Fermentation Broth on Mycelia Growth of Sclerotinia sclerotiorum

[0012] Three treatments were set up: treatment 1 was control (ck) potato sucrose (PS) liquid medium 300ul; treatment 2 was 150ul fermentation broth + 150ul potato sucrose (PS) liquid medium; treatment 3 was 300ul fermentation broth. Each treatment was repeated 3 times, respectively coated on the PSA medium, and then used a 5mml puncher to get the bacterium cake at the edge of the colony of Sclerotinia sclerotiorum, and then inoculated it on the PSA medium that had been coated In the center of the plate, after culturing at 28°C for 72 h...

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Abstract

The invention discloses a bacterial strain with antagonistic effect on Sclerotinia sclerotiorum of Chinese cabbage. , Perinatal flagella. Gram-positive, spores 0.7-0.9 × 1.1-1.4 microns, oval. The surface of the colony is rough and opaque, white or slightly yellow, and it was slightly yellow after 72 hours of shaking bacteria in potato glucose liquid medium; it was preserved in the China Center for Type Culture Collection on April 26, 2018, and the preservation number is CCTCC NO: M 2018236 . The strain of the invention is simple to manufacture, low in cost, non-toxic, harmless and free from environmental pollution, and is beneficial to safely and effectively inhibiting the growth of Chinese cabbage Sclerotinia sclerotiorum mycelium, and is beneficial to the green cultivation of Chinese cabbage, and protects farmland ecosystems.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a bacterial strain with antagonistic effect on Sclerotinia sclerotiorum of Chinese cabbage and its cultivation method and application. Background technique [0002] Bacteria of the genus Paenibacillus have been isolated from a variety of environments, and many species are associated with humans, animals, plants, and the environment. Most of the bacteria of the genus Paenibacillus are found in the soil, and are usually plant root growth-promoting bacteria. These bacteria of the genus Paenibacillus can promote plant growth and can be used in agricultural production. Some Paenibacillus bacteria produce antibacterial compounds that are useful in medicine or pesticides, and others are used to produce enzymes for bioremediation or to produce valuable chemicals. Paenibacillus polymyxa can produce polypeptide antibiotics, antagonistic proteins, enzymes, plant hormones, flocculants and o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/25A01P3/00C12R1/01
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 雷娟利钟新民李必元岳智臣陶鹏赵彦婷
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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