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Biocontrol strain YKY01 for preventing and treating aromatic tobacco black shank and anti-biological inoculant of biocontrol strain YKY01

A technology for bio-control strains and bio-control agents, applied in the directions of biocides, fungicides, biocides, etc., can solve the problems of high cost, difficulty, and high chemical pesticide residues, and achieve growth inhibition, increase microbial activity, and protection. Effects of farmland ecosystems

Inactive Publication Date: 2013-11-27
YUNNAN ACAD OF TOBACCO AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The purpose of the present invention is to provide a biocontrol strain for preventing and treating oriental black shank in order to solve the problems that the prevention and treatment of oriental tobacco black shank is difficult and costly in organic cultivation, and chemical pesticides and pesticide residues are not suitable for use in current agricultural production. YKY01 and its biocontrol agents

Method used

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  • Biocontrol strain YKY01 for preventing and treating aromatic tobacco black shank and anti-biological inoculant of biocontrol strain YKY01
  • Biocontrol strain YKY01 for preventing and treating aromatic tobacco black shank and anti-biological inoculant of biocontrol strain YKY01
  • Biocontrol strain YKY01 for preventing and treating aromatic tobacco black shank and anti-biological inoculant of biocontrol strain YKY01

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of biocontrol agent

[0032] a. Transplant the strain YKY01 into a petri dish for one-loop activation, culture at 33°C for 72 hours; the NA solid medium formula in the petri dish is: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, agar 15.0g , Distilled water 1L, PH value 7.3;

[0033] b. Take a ring of activated bacterial lawn and transfer it into a 300ml 500ml triangular bottle. The formula is: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, distilled water 1L, pH 7.3 NA culture solution, at 175 revolutions / min, cultivate for 72 hours at 33 ℃ to obtain the first-level fermentation broth;

[0034] c. Inoculate the first-level fermentation bacteria liquid in the seed tank at the same ratio of inoculation amount 1:150 in NA culture liquid as in step b. The temperature of the seed tank is 33℃ or 34℃ or 35℃, and the fermentation is 48 hours. Afterwards, obtain the bacterial seed liquid for secondary fermentation;

[0035] d. Inoculate the ...

Embodiment 2

[0037] Example 2: Plate Antagonism Test

[0038] According to Fang Zhongda's "Plant Disease Research Method", the plate-to-hold culture test method was used to evaluate the inhibitory effect of YKY01 bacteria on the black shank pathogen of oriental tobacco. At the same time, the statistical results of the sterilized distilled water control group when the pathogenic mycelia were overgrown on the plate As follows, the growth inhibition rate of YKY01 on the black shank pathogen of oriental tobacco is 91.50% (shown in Table 1). It shows that the YKY01 strain has a significant inhibitory effect on the black shank of oriental tobacco.

[0039] Table 1 Inhibitory effect of YKY01 on black shank pathogen of oriental tobacco and related pathogens

[0040]

Embodiment 3

[0041] Example 3: Greenhouse control test of oriental tobacco black shank

[0042] Set up three treatments, treatment 1 is the sterile water control group, treatment 2 is the YKY01 bacterial agent diluent, and treatment 3 is the fungicide carbendazim (Nanjing Boshibang Chemical Technology Co., Ltd.). Transplant the oriental tobacco floating plate seedlings into the soil infected with the black shank pathogen of the oriental tobacco. When transplanting, use YKY01 microbial agent 1:30 times to dip the roots, and then transplant the roots; 7 days after the crops are transplanted, use YKY01 microbial agent 1: Roots are irrigated with 30 times bacterial solution, and 10 crops per liter of liquid are irrigated; 90 days after crops are transplanted, roots are irrigated with YKY01 bacterial agent 1:30 times bacterial solution, and 10 crops are irrigated per liter of liquid. Other clear water control and carbendazim pesticide treatments are the same as the above methods and dosages. Ever...

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Abstract

The invention discloses a biocontrol strain YKY 01 for preventing and treating aromatic tobacco black shank and an anti-biological inoculant of the biocontrol strain YKY01, belonging to the technical field of bio-pesticides. The preservation number of the strain YKY01 is CGMCC (China general Microbiological Culture Collection) NO.7723. The anti-biological inoculant is obtained by the following steps of transplanting the strain YKY01 to a culture dish for activating, and culturing for 72 hours at 33 DEG C; transferring the activated lawn into 300ml of NA culture solution which is contained in a 500ml triangular flask, culturing for 72 hours at the rotation speed of 175 revolutions / min under the temperature of 33 DEG C to obtain primary fermentation liquor; inoculating the primary fermentation liquor into the NA culture solution in a seed tank according to an inoculation amount proportion of 1:150, thereby obtaining strain liquor for secondary fermentation after fermenting for 48 hours with the temperature of the seed tank of 32 DEG C-35 DEG C; and inoculating the strain liquor for the secondary fermentation to a fermentation tank for fermentation according to the inoculation amount proportion of 1:15, fermenting for 120 hours at the temperature of 32 DEG C-35 DEG C, and storing the strain liquor as biocontrol liquor, centrifuging for 3 minutes at 1500 rpm, and preparing with special-purpose preservative fluid to form active fungicide, so that the total concentration of viable bacteria in the fungicide is above1*1011 FU / ml. According to the biocontrol strain YKY 01 for preventing and treating aromatic tobacco black shank and the anti-biological inoculant of the biocontrol strain YKY01, the cost is low, the successive cropping obstacle of the aromatic tobacco black shank can be effectively prevented, and the yield and the quality are improved.

Description

Technical field: [0001] The invention relates to a biocontrol strain YKY01 for preventing and treating black shank disease of oriental tobacco and its biocontrol bacteria agent, belonging to the technical field of biological pesticides. Background technique: [0002] Oriental tobacco, also known as Turkish tobacco and Oriental tobacco, is originally produced in the countries along the Mediterranean Sea and is a special type of tobacco from Nicotian atobacum. Because oriental tobacco has the characteristics of rich aroma and pure taste, it has special application value in tobacco products. It is one of the important raw materials for the production of mixed, external and oriental cigarettes and pipe tobacco. In particular, blended cigarettes have the characteristics of strong, strong flavor, wide range of materials, and can effectively reduce the tar content to reduce the harm of smoking to human health. They are favored by smokers and become a popular type of cigarettes in the wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 张晨东孔垂思肖植文余磊邱学礼杨谨付斌余小芬赵建荣徐兴才侯朝祥
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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