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Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and application

A technology of Pantoea agglomerans and endophytic bacteria, which is applied in the field of plant protection, can solve the problems of biological agents that have not yet been seen, and achieve good growth-promoting effect and good comprehensive properties

Inactive Publication Date: 2014-04-09
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] According to the literature search, there is no public report on the use of the endophytic bacterium Pantoea agglomerans strain 1-7 to prepare biological agents and apply them to field control of bacterial soft rot of konjac and clubroot of cruciferous vegetables.

Method used

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  • Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and application
  • Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and application
  • Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment one: The inhibitory activity of biocontrol agent 1-7 to konjac bacterial soft rot

[0020] (1) In vitro antibacterial test of biocontrol strains

[0021] 1. Test materials:

[0022] 1.1 Bacteria to be tested: Pantoea agglomerans 1-7.

[0023] 1.2 Pathogens tested

[0024] Plant pathogenic bacteria (konjac bacterial soft rot, Chinese cabbage soft rot, calla lily soft rot, rice bacterial blight, rice bacterial streak), pathogenic fungi (Phytophthora capsici, Panax notoginseng root rot, Tobacco black shank bacteria) are shown in Table 1, all provided by the Bacteria Laboratory of the National Engineering Center for Biodiversity and Disease Control, Yunnan Agricultural University.

[0025] 1.3 Reagents and media for testing

[0026] NA medium (5g peptone, 3g beef extract, 1g yeast extract, 10g sucrose, 17g agar, 1000mL water, pH7.0); PDA medium (200g potato, 15g agar, 15g glucose, 1000mL water).

[0027] 2. Test method

[0028] plate stand-off

[0029] 1) ...

Embodiment 2

[0035] Embodiment two: Biocontrol bacterium agent 1-7 is to the growth-promoting action effect of konjac

[0036] 1-7 Preparation of biological agent:

[0037] Biocontrol bacterial strains 1-7 were cultured in NB culture medium at 28°C with shaking at 150 rpm for 36 h-48 h, then centrifuged at 10,000 rpm for 5-10 min, discarded the supernatant, kept the cells, added the cell diluent, and pressed The mass ratio is 1:40 to prepare the bacterial agent, and the number of viable bacteria in the finished bacterial agent is 5X109 cfu / ml, the cell dilution is 0.01 mol / L phosphate buffer, containing 0.01% (V / V) CMC (carboxymethyl cellulose) solution (film-forming agent) and 0.001 mol / L Tween 20 ( nonionic surfactants).

[0038] 1-7 Konjac bulbs treated with biocontrol agents:

[0039] Take healthy konjac bulbs, the size is 50g / piece, and use biocontrol agent 1-7 100 mL (about 10 8 CFU / mL) soaking konjac bulbs, 50 pieces / treatment, the soaking time is 1h, fish out, each bulb is pla...

Embodiment 3

[0044] Embodiment three: the control effect of biocontrol agent 1-7 to konjac soft rot

[0045] 1.1. Biocontrol agent for test: biocontrol agent 1-7

[0046] 1.2. Test crop: Fuyuanhua konjac

[0047] 1.3. Control object: Konjac bacterial soft rot

[0048] 1.4. Test location: Konjac planting base in Fuyuan County, Yunnan Province, with medium fertility. The plot is a 4-row area with an area of ​​30 square meters. There are 3 replicates for each treatment, arranged randomly, a total of 9 plots, and protection rows are set around. Standardized management of high-quality cultivation measures.

[0049] The experiment consisted of three treatments: biocontrol agent treatment, clean water control, and pesticide control. The method of pesticide application adopts the root irrigation method, and the root of each konjac is poured with 100 mL of pesticide solution. The first pesticide is applied before the initial stage of the disease, and the interval is one week. The pesticide is ...

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Abstract

The invention relates to a Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and an application, which belongs to the biology technical field. The Pantoea agglomerans1-7 is preserved in the China General Microbiological Culture Collection Center on May 28th, 2012 with the preservation number of CGMCC No.6160. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 is used for preparing the preparations used for preventing and treating bacterial soft rot of konjak and clubroot disease on cruciferous vegetable club root in fields. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 has the advantages of high efficiency, no toxicity, safety and no residue, the bacterial strain1-7 has the characteristics of good disease control, better growth promotion effect, better comprehensive characters and easy industrial production, and the Pantoea agglomerans1-7 can be applied to preparation of preparations for controlling cruciferous vegetable club root in fields.

Description

Technical field: [0001] The invention relates to a konjac endophytic bacterium Pantoea agglomerans strain 1-7 and application thereof, belonging to the technical field of plant protection. Background technique: [0002] Konjac is a perennial herbaceous plant of the Araceae family Konjac. It is a plant that can extract a large amount of glucomannan. Konjac field planting has a high yield and good economic benefits. It is cultivated on a large scale in southwestern provinces of my country and has become the main economy in this area. Crops are also one of the effective ways to adjust the agricultural structure in the region, get rid of poverty and become rich, and revitalize the local economy. Bacterial soft rot of konjac is very serious all over the world. It occurs during the field growth period and the anniversary of the storage period. After the konjac plant is infected with soft rot, the leaves or bulbs become soft, black and rotten, and have a foul smell. Bacteria-carryi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/01
Inventor 姬广海魏兰芳王永吉何月秋李成云
Owner YUNNAN AGRICULTURAL UNIVERSITY
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