50results about How to "Strong digestion" patented technology

The invention belongs to the technical field of environment protection and discloses a compound microbial bacterial preparation and application thereof. The compound microbial bacterial preparation consists of fermentation liquor of four bacteria, i.e. Bacillus licheniformis, Pseudomonas putida, Enterobacter cloacae and Candida valida. The compound microbial bacterial preparation provided by the invention has strong digestion power for various types of cellulose, proteins and fat macromolecules. The addition of the compound microbial bacterial preparation into excess sludge and / or kitchen waste can quicken enrichment of methanogen, shorten anaerobic digestion time and improve methane yield. Moreover, the synergetic effect of the four microbial strains is superior to the effect of any one, two or three strains of microorganisms.

The invention discloses improved special fattening feedstuff for wild pigs, which comprises the following ingredients in percentage by weight: 15 to 25 of corns, 5 to 15 of wheat bran, 3 to 7 of bean dregs, 20 to 40 of chicken manure, 10 to 30 of pumpkin, 3 to 7 of carrots, 1 to 3 of fish meal, 1.7 to 3.3 of bone meal, 1 to 3 of shell powder, 0.3 to 0.7 of common salt, 1 to 2 of dried orange peel and 3 to 7 of hawthorn. The feedstuff has the advantages of low cost, good palatability and the like.

The invention provides a construction method of an RNA methylation sequencing library. The construction method comprises the following steps: (S1) carrying out fragmentation on an RNA sample, so as to obtain fragmented RNA; (S2) carrying out co-immunoprecipitation on the fragmented RNA by virtue of an RNA methylation specific antibody, so as to obtain precipitates; (S3) digesting the precipitates by virtue of protease, so as to obtain a digestion product; (S4) extracting RNA from the digestion product, and carrying out reverse transcription on extracted RNA, so as to obtain cDNA; and (S5) constructing a sequencing library by virtue of the cDNA, so as to obtain the RNA methylation sequencing library. The digestion of protease is stronger than the competitive elution of analogues, so that the yield of RNA can be increased, the noise generated due to the introduction of methylation site analogues is avoided, the problem of high noise of constructed libraries in the prior art is solved, the library construction quality is improved, and therefore, the utilization rate of valid data output by the sequencing library is increased.

The invention discloses a south high moisture and hot region beef cattle rapid fattening culture method comprising four stages: beef cattle rapid fattening early stage preparation; beef cattle rapid fattening adaptation stage; beef cattle rapid fattening transition period; beef cattle rapid fattening later stage; the method also comprises a beef cattle cooling process, a beef cattle dermatopathy prevention process and a beef cattle enterogastritis prevention process. The method employs store cattle of 12-18 months, preferably uses castrated bulls, and the poise characteristics comprise the following: 300-400kg body weight, healthy growth, big physique, strong arms and legs, and anosis. The beef cattle cultured by the method can have fullness body muscles after fattening, the fur is bright, and average daily weight growth is obvious.

The invention discloses a drug for treating elderly abdominal distension. The drug comprises 6-14 parts of ginseng, 10-20 parts of bighead atractylodes rhizome (fried), 5-15 parts of licorice (processed with honey), 10-20 parts of rhizoma cyperi (processed with vinegar), 12-18 parts of citron, 6-14 parts of amomum fruit, 13-17 parts of elecampane, 10-20 parts of fructus aurantii (stir-baked with bran), 14-22 parts of pericarpium citri reticulatae, 25-35 parts of semen raphani (fried), 8-16 parts of medicated leaven (fried), 10-20 parts of malt (fried), 14-22 parts of hawthorn (fried), 8-16 parts of gizzard pepsin (fried), 9-15 parts of inula flower, 26-34 parts of ruddle, 5-15 parts of ginger processed pinellia, 9-21 parts of pawpaw, 9-21 parts of dendrobe, 14-26 parts of yam, 10 parts of ginger and 15 parts of jujube. The preparation method of the drug comprises the following steps: firstly, decocting ruddle in a boiler to boil, wrapping the inula flower in cloth, mixing the components, adding 3 times of water to soak the components for 30 minutes, and then, putting the components in the boiler, boiling the components through strong fire, and continuing to decoct the components for 30 minutes through slow fire, removing residues, extracting juice, continuously decocting the components for twice, mixing the decoction obtained in twice to obtain the drug. The components mutually act, thereby both curing the stomach and protecting the stomach, treating both manifestation and root cause of disease, facilitating to create gastrointestinal digestion and promoting reinforcement of gastrointestinal motility.

The present invention relates to a freshness preserved fish stewing seasoning which includes the following raw materials in parts by weight: 2-5 parts of Chinese prickly ash, 1-3 parts of Chinese wolfberry fruits, 2-4 parts of bay leaves, 0.3-1.5 parts of cloves, 0.2-1 part of alpinia hainanensis, 0.3-0.6 part of star anises, 1-4 parts of dried gingers, 0.2-0.6 part of ligusticum chuanxiong, 0.2-0.5 part of hoodshaped alocasia rhizome, 0.1-0.4 part of pepper, 0.3-0.6 part of dried tangerine peels, 0.2-0.6 part of rhizoma kaempferiae, 0.2-0.6 part of radix angelicae dahuricae, 0.1-0.3 part of nutmeg, 0.3-0.5 part of fructus amomi, 2.5-4 parts of fennel, 0.6-1.2 parts of pungent pepper (a kind of Chinese prickly ash), 0.4-1.2 parts of cassia barks, 0.3-1 part of hedychium spicatum rhizome, 0.4-1 part of liquorice, 0.2-0.5 part of cumin and 0.6-0.9 part of fructus tsaoko. The freshness preserved fish stewing seasoning is long in fresh fragrance and delicious taste, and removes fishy smell and inhibits impurity, so that the stewed fish flesh is fresh, tender and tasty, the nutrients are easier to be absorbed by human body and the fish stewing seasoning is beneficial to human body health.

The invention provides a fast-growing big broiler feeding method. The method is characterized in that corresponding feeding methods are adopted for different stages according to requirements for different sale weights; three-stage feeding is adopted when sale weight is smaller than or equal to 1.6 kg; three-stage feeding is adopted when sale weight is 1.7-2.4 kg; four-stage feeding is adopted when sale weight is 2.5-3.0 kg; five-stage feeding is adopted when sale weight is 3.1-4.0 kg. According to the method, feed nutritive indexes and feeding methods are set for five broiler sale weight ranges correspondingly, nutrient requirements of fast-growing big broilers at different growth stages can be met, expected marketing weight and market age are realized, and adjustability of broiler sale weight is realized.

The invention relates to the technical field of livestock breeding, and particularly discloses a feeding method for ruminant as follows: parasites are expelled from the ruminant after the ruminant is weaned, the stomach of the ruminant is invigorated within 3 days after the parasite expelling, and each ruminant with empty stomach is fed with a nutritional sustained-release pellet. The feed and the consumption of the weaned ruminant are as follows: 4 to 10 kg per ruminant each day of graminaceous silage, 4 to 10 kg per ruminant each day of fresh graminaceous feed, and 0.5 to 3 kg per ruminant each day of concentrated feed. Compared with the prior art, the feeding method is used for feeding the ruminant, so as to complement microelements, accelerate the absorption of the microelements, then consequently accelerate the absorption of nutrient in the feed, enhance the digestion and reduce the feeding cost.

The invention discloses an ecological chicken raising method which comprises the following measures: a leeward sun-facing mountain slope with sufficient water sources is selected, and a fence is arranged on the mountain slope, and a piece of land is enclosed and serves as a chicken farm, a natural stream flows through the chicken farm or a water channel is artificially dug to serve as a water source of drinking water of chickens or a water feeding device is artificially set; a fruit tree is planted in the chicken farm, and forage grass is planted on the ground of the chicken farm, a pluralityof grass houses are arranged beside the fence at one side and used for holding the chickens at night; the chickens freely move in the chicken farm in the daytime and are driven to the grass houses byworkers at night, and a plurality of automatic feeding devices are arranged between the forest trees at intervals; and a plurality of sand pits for the chicken sand bath are further arranged in the chicken farm. The ecological chicken raising method has the advantages that firstly, the fruit trees and the forage grass can simultaneously digest the chicken manure; secondly, the manual workload is reduced; thirdly, a plurality of sand pits for the chicken sand bath are further arranged in the chicken farm, and the habits and the health of the chickens are ensured; fourthly, fruits and the chickens can be sold.

The invention discloses a mild cell digestive enzyme cell digestive juice for stem cells. The digestive juice is composed of components at the following concentrations, according to a concentration unit mg / L: dipotassium hydrogen phosphate at 50 to 70, potassium chloride at 350 to 450, sodium hydrogen carbonate at 300 to 400, sodium chloride at 7,000 to 9,000, sodium hydrogen phosphate heptahydrate at 80 to 100, glucose at 1,000 to 2,000, EDTA-4 Na at 200 to 500, stem cells mild digestive enzyme at 1 to 120 and the balance of water. The mild digestive enzyme cell digestive juice for the stem cells, disclosed by the invention, has better digestion capacity on the cells than that of a traditional pancreatic enzyme, and the cells can be completely digested within 3 minutes. The mild digestiveenzyme cell digestive juice has less damage to the cells in a cell digestion process; after digestion is completed, the cells are inoculated, so that the cell viability is high and the growth state is good. The mild digestive enzyme cell digestive juice for the stem cells does not need to be stored at -20 DEG C, and can maintain the use effect at 2 to 8 DEG C within 12 months.

The invention belongs to the technical field of aquaculture, and relates to a feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and a preparation method of the feed additive for preventing and treating the bacterial gill-rot disease of the ictalurus punctatus. The feed additive for preventing and treating the bacterial gill-rot disease of the ictalurus punctatus is prepared from the following components in parts by mass: 35 to 45 parts of ginger juice, 26 to 32 parts of lalang grass rhizome, 26 to 32 parts of cactus juice, 25 to 33 parts of acorus calamus, 25 to 32 parts of onion juice, 22 to 30 parts of portulaca oleracea, 22 to 28 parts of dark plum, 22 to 28 parts of dried orange peel, 20 to 28 parts of Chinese gallnut, 18 to 28 parts of almond powder, 16 to 26 parts of euphorbia helioscopia, 16 to 26 parts of acanthaceae sericocalyx chinensis, 10 to 18 parts of fish oil and the like. According to the feed additive and the preparation method disclosed by the invention, the occurrence of the bacterial gill-rot disease of the ictalurus punctatus can be effectively prevented; in addition, the bacterial gill-rot disease of the ictalurus punctatus can be effectively treated.

The invention belongs to the field of medical science, and relates to a mouse skin digestion complex enzyme and a method for isolating micro-immunity Treg cells from mouse skin. The mouse skin digestion complex enzyme is prepared by a RPMI medium, a HEPES buffer solution with a final concentration of 1%, fetal bovine serum with the final concentration of 10%, a penicillin / streptomycin double-antibody solution with the final concentration of 1%, type 11 collagenase with the final concentration of 2 mg / ml, hyaluronidase with the final concentration of 0.5 mg / ml, and deoxyribonuclease with the final concentration of 2 mg / ml. The method can efficiently obtain the micro-immunity Treg cells from a limited size mouse skin tissue, which facilitates cytological and molecular studies on skin-specific Treg cells, and also provides a reference for separating other micro cells in the skin. .

The invention discloses an enzyme composition for livestock feed in the technical field of livestock breeding. The enzyme composition comprises the following components: sweet wormwood, folium artemisiae argyi, houttuynia cordata and groundsel. The invention further provides a method for preparing enzymes from the enzyme composition for livestock feed. The method comprises the following steps: mixing the folium artemisiae argyi, sweet wormwood, houttuynia cordata and groundsel, obtaining a drug mixture, and grinding for later use; and preparing 1 part of brown sugar, 1-4 parts of the drug mixture and 5-10 parts of clean water; adding the clean water into a sealed container, adding the brown sugar to stir and melt, adding the drug mixture into the clean water to be uniformly stirred, sealing, fermenting in a cool and dry place for 90-100 days, filtering, and taking the filtrate so as to obtain the enzymes. The enzyme for livestock feed prepared from the enzyme composition disclosed by the invention is additive-free, is prepared by fermenting pure natural plants, and can achieve the effects of enhancing the digestive abilities of livestock and poultry such as broilers, pigs and the like after eating, improving the immunity, further avoiding diseases and improving the survival rate of the livestock and poultry fed by the livestock feed.

The invention relates to the technical field of chicken raising and discloses an ecological layer breeding method. The method comprises the steps as follows: within 40-60 days after birth of chicks, the temperature in a chick house is kept at 22-36 DEG C, the humidity is kept at 55%-70%, the illumination time is 12-24 h, chicks are fed with water before taking food, and the water contains 5%-8% glucose; after 60 days, raised adult chickens are placed in a mountain to be bred, Chinese herbal medicines comprising dandelion, plantain herb and astragalus membranaceus are planted in the chicken raising range of the mountain, and adult chicken feed comprises raw materials in parts by weight as follows: 1-3 parts of ferrous sulfate, 1-4 parts of zinc sulfate, 30-45 parts of corn flour, 5-13 parts of chaff, 5-13 parts of millet, 2-6 parts of bean dregs, 15-25 parts of sweet potato vines, 0.4-0.8 parts of red yeast rice, 0.3-0.6 parts of table salt, 4-10 parts of carrots, 2-6 parts of fish meal, 1-3 parts of garlic powder, 5-13 parts of peanut seeds and 1-5 parts of river clamshell meal. Layers raised with the method contain rich calcium, iron, zinc and protein, the content of cholesterol is reduced, and eggs are more nutritious. Besides, no additives or antibiotics are added in the whole raising process and no harm is caused to the human body.

The invention belongs to the field of medicine, and relates to human skin digestion complex enzyme and a method for isolating micro-immune cell Treg cells from human few full-thickness skin; the human skin digestion complex enzyme is a RPMI medium, a HEPES buffer solution with a final concentration of 1%, fetal bovine serum with the final concentration of 10%, a penicillin / streptomycin double-antibody solution with final concentration of 1%, type 4 collagenase with the final concentration of 4 mg / ml, hyaluronidase with the final concentration of 0.5 mg / ml, and deoxyribonuclease with the final concentration of 2 mg / ml; the method for isolating micro-immune cell Treg cells from human few full-thickness skin includes the following steps: true epidermal separation; digestion with a human skin digestion complex enzyme solution; performing filtration; and performing analytical analysis using flow cytometry. The invention facilitates the research of cytological and molecular studies on skin-specific Treg cells, and can also provide some reference for isolating other micro-cells in the skin.

The invention discloses a method for preparing a vegetarian dried meat floss product from cordyceps millitaris fermented glycine max dregs. A glycine max processing byproduct, namely glycine max dregs, is adopted as a raw material, through solid fermentation, flavor blending and frying with cordyceps millitaris, contents of two soy isoflavones, namely daidzein and glycitein, can be remarkably increased, the content of insoluble cellulose can be reduced, and the vegetarian dried meat floss product which is rich in aglycone isoflavones, proteins and edible fungi, is cotton-like and fluffy and has sensitive, physical and chemical and microorganism indexes similar to those of dried meat floss is prepared. The product prepared by using the method is "natural, nutrient and low-grease", and expectation of different customers on nutrient and healthy foods can be met. Solid fermentation used in the method is a traditional and modern biological technology, is efficient and low-carbon, good in applicability and simple to operate, and is free of secondary sterilization; the cordyceps millitaris can be used as an edible fungus, the mycelia of the cordyceps millitaris is rich in nutrition, and the flavor and the drawing feeling of the product can be also improved. By adopting the method, the nutrition and functional value of the vegetarian dried meat floss leisure food can be effectively intensified, and great significances in increasing additional values of the vegetarian dried meat floss product and promoting comprehensive utilization of the glycine max dregs can be achieved.

The invention discloses a method for extracting active components of skin stem cells. The method comprises the steps of adding human skin tissues to a normal saline mixed digestive fluid of a type I collagenase and a type II collagenase added with a PMSF protease inhibitor for constant temperature shaking table culture; stopping digestion by a culture medium M199 containing 10% by volume of fetal calf serum; performing centrifuging and precipitate collection; performing cell re-suspension of normal saline to obtain compound skin stem cells; and screening out Dlk1<+> / Scal<-> skin stem cells by applying magnetic bead marked Dlk antibody and Scal antibody. The purity of the Dlk1<+> / Scal<-> skin stem cells extracted with the method can reach 95.8%; and the skin stem cells are large in quantity, high in cell survival rate and high in activity, are applied to drugs for treating hair loss, and have a remarkable hair growth effect.

The invention discloses a feed capable of improving the survival rate of river crabs. The feed is prepared from the following raw materials: puffed corn flour, rice bran, worm meal, shrimp head meal,fish meal, chicken meal, egg-shell meal, lentinus edodes powder, seaweed meal, Chinese cabbage powder, carrot powder, phosphoryl oligosaccharides, ecdysterone, chitosan, a Chinese herbal medicine additive and vegetable oil. The feed capable of improving the survival rate of river crabs provided by the invention is reasonable in formula, rich and balanced in nutrients, good in palatability and easyfor river crabs to digest and absorb, and the river crabs fed with the feed are effectively improved in constitution and have high survival rate, fast growth and development and good meat quality, sothe economic benefit in culturing the river crabs is effectively improved.

The invention relates to prochilodus scrofa advanced fry mixed feed, and further relates to a preparation method of the prochilodus scrofa advanced fry mixed feed. The prochilodus scrofa advanced fry mixed feed is prepared from the following components in parts by weight: 20 to 30 parts of ulex europaeus powder, 4 to 13 parts of cicada powder, 6 to 15 parts of alfalfa seed powder, 5 to 14 parts of thin sheets of bean curd, 4 to 13 parts of sweet photo leaves, 6 to 14 parts of shepherdspurse herb, 5 to 16 parts of water celery, 4 to 14 parts of osmunda cinnamomea, 5 to 14 parts of arrowhead, 6 to 16 parts of clam powder, 3 to 9 parts of plantain herb, 2 to 9 parts of endothelium corneum gigeriae galli, 3 to 10 parts of fish oil, 1 to 6 parts of honey, 2 to 6 parts of olive oil, and 1 to 4 parts of compound vitamin. The prochilodus scrofa advanced fry mixed feed is provided according to an energy metabolism mode and nutritional requirement of prochilodus scrofa advanced fries, can completely replace fish meal, after the prochilodus scrofa advanced fry mixed feed is fed to the prochilodus scrofa advanced fries, the survival rate of the prochilodus scrofa advanced fries is high, the feed coefficient is low, and the specific growth rate is high.

The invention discloses an abalone biological fermentation feed capable of inhibiting vibrio, and relates to the field of biological feeds. The abalone biological fermentation feed capable of inhibiting vibrio is composed of the following components by weight: 25-35 parts of starch, 25-35 parts of rape seed cake, 12-18 parts of marine fish bone meal, 16-22 parts of sea meal, 8-12 parts of stone powder, 2-4 parts of calcium dihydrogen phosphate, 0.15-0.25 part of compound enzyme, 0.12-0.25 part of vitamin E, 0.13-0.15 part of bacillus safensis and 0.06-0.12 part of lactobacillus acidophilus. According to the abalone biological fermentation feed, the starch, the rape seed cakes, the marine fish bone meal, the sea meal, the mountain flour, the monocalcium phosphate, the compound enzyme, the vitamin E, the bacillus safensis and the lactobacillus acidophilus are used as raw materials for preparing the abalone biological fermentation feed capable of inhibiting the vibrio; the abalone biological fermentation feed is rich in protein, amino acid and other nutrient substances easy to digest and absorb, improves the disease resistance of abalones, is high in digestion ability of abalones, can avoid impurities generated during feeding, ensures the water quality, inhibits abalone infection, and is beneficial to ecological and healthy culture.

The invention provides selenium-rich matcha ice cream powder which is prepared from the following components: 30-50wt% of white granulated sugar, 30-50wt% of skim milk powder, 4-15wt% of maltodextrin,1-15wt% of selenium-rich matcha powder, 4-15wt% of monoglyceryl ester and 4-15wt% of non-dairy creamer. The ice cream powder is prepared from organic selenium elements and the matcha powder at the same time, and the ice cream powder with a health care function is produced; an ice cream prepared from the ice cream powder has the characteristics of cool, refreshing and rich flavor of the traditional ice cream and is also prepared from a great amount of the organic selenium elements; and meanwhile, the matcha is prepared from trace elements including tea polyphenol, caffeine, vitamins, amino acid and phospholipid and the like, has the effects of facilitating human digestion and regulating fat metabolism and has very strong functions of eliminating greasy food, helping digestion and the like.

The invention discloses an antibiotic-free selenium-rich folium mori fodder special for flocks and herds. The fodder contains the following components in parts by weight: 180-300 parts of corn flour, 95-140 parts of bean pulp, 50-80 parts of garden stuff cellulose powder, 6-11 parts of multi-vitamins, 1-2 parts of cordate houttuynia, 2-3 parts of probiotics, 0.1-0.3 part of talcum powder, 5-10 parts of pearl powder, 1-2 parts of vinegar, 5-10 parts of artemisia caespitosa, 153-265 parts of folium mori, 20-50 parts of baking soda and 5-10 parts of shrimp meal. The antibiotic-free selenium-rich folium mori fodder special for flocks and herds provided by the invention contains no antibiotic and has effective preventing and treating functions for diseases including food-mouth inflammation, lung disease and intestine disease of flocks and herds.

The present invention discloses a feed having good palatability and balanced nutrition for pigs in a fattening period. The feed comprises the following raw materials in parts by weight: 25-45 parts of corns, 5-10 parts of rapeseed cakes, 5-12 parts of sweet potato stalks, 3-10 parts of carrot powder, 4-8 parts of sorghum powder, 10-20 parts of soybean meal, 2-8 parts of broussonetia papyrifera leaf powder, 1-6 parts of sweet potato powder, 1-4 parts of fermented biological proteins, 4-10 parts of millet flour, 2-6 parts of corn germ meal, 1-4 parts of a Chinese herbal medicinal additive, 1-2 parts of membrane coated myricetin, 0.5-1.5 parts of soybean lecithin, 1-3 parts of stone powder, 0.5-1.5 parts of malt selenium, 1-4 parts of spirulina powder, 1-4 parts of red chili powder, 0.1-0.6 part of vitamin, and 1-3 parts of an attractant agent. The feed is comprehensive and balanced in nutrition, and good in oxidation resistance and palatability, can effectively remove free radicals in the body, maintain the intestinal microecological balance, reduce intestinal diseases, enhance the digestion and absorption levels of the pigs, and improve the economic benefits of the pig breeding.

The invention discloses a drug capsule for preventing and treatingchronic diarrhea. The drug capsule is prepared from main drug components and a capsuleadjuvant material, and is characterized in that the main drug components compriseextracts of rhizoma atractylodismacrocephalae, rhizoma atractylodis, rhizoma zingiberis, poriacocos, radix glycyrrhizaepreparata, radix saposhnikoviae, pericarpiumcitrireticulatae, radix paeoniae alba, myristicafragrans, fructus evodiae, fructus psoralea, radix angelicae, raw coix seeds, caulis perllae, wrinkled gianthyssopherb, semen euryales,stir-baked rhizoma dioscoreae and fructus schizandrae. The drug capsule can obviously reduce the generation and development of chronic diarrhea, has the functions of improving enteric internal environment, promoting intestinal mucosarepair and regeneration, resisting and defending invasion of harmful microorganisms and absorption of water by intestinal tract, and improving the digestive system, and has the effects of invigorating the spleen, tonifying the kidney, strengthening and consolidating body resistance and treatingboth the symptoms and root causes of diseases.

The invention discloses a nondestructive in-vivo squid DNA sample acquisition method. By friction of a squid mantle cavity, tissue cells are obtained through scraping to serve as a sample for DNA extraction. The DNA extraction method is an improved CTAB method, and by strong denaturation and complexation of pyrolysis buffer solution, phenol-chloroform-isoamyl alcohol repeated extraction and chloroform-isoamyl alcohol-petroleum ether reaction, high protein digestion capacity and removal of impurities including phenols, saccharides and the like with serious interferences on enzyme functions arerealized while high yield is achieved. In addition, high extracted DNA molecular weight is realized, evident degradation is avoided, nucleic acid completeness is kept, a measured OD value range is 1.6-1.9, and high DNA purity is realized.

The invention discloses a method for separating and cultivating endothelial cells of common carotid arteries of SD (Sprague Dawley) rats. The method includes steps of 1), incising the common carotid arteries of the SD rats under sterile conditions; 2), cutting and segmenting the common carotid arteries, then stripping outer membranes from the common carotid arteries in PBS (poly butylenes succinate) liquor, longitudinally cutting the common carotid arteries, downwardly flatly spreading inner membranes in containers filled with digestive enzyme liquor and enabling the inner membranes of the common carotid arteries to be in sufficient contact with the digestive enzyme liquor in the containers; 3), vibrating the containers to allow the endothelial cells to fall off, removing the common carotid arteries after digestion is completed, adding fetal bovine serum into the containers and uniformly stirring the fetal bovine serum to obtain mixed liquid; 4), centrifuging the mixed liquid, removing supernatants to obtain the endothelial cells of the common carotid arteries, cultivating the endothelial cells of the common carotid arteries in endothelial cell cultivation media, transferring the endothelial cells to cultivation tanks to cultivate the endothelial cells after the endothelial cells reach log phases and then carrying out generation cultivation on the endothelial cells. The method has the advantages of high survival rate, little cell injury, economic efficiency, practicality, simplicity, feasibility and applicability to mass production in factories and the like.

The invention relates to a laying duck feed and a processing method thereof. The feed comprises the following components in percentage by weight: 74 percent of middling powder, 7 percent of shell powder, 3 percent of cotton dregs, 2.625 percent of corn germ cake, 6 percent of meat and bone meal, 5 percent of fish meal, 1.0 percent of lard oil, 0.35 percent of table salt, 0.025 percent of methionine, 0.2 percent of compound biological enzyme preparation and 1.0 percent of premix. The processing method comprises the following steps of: pulverizing and / or grinding the components in the feed, adding each raw material into a stirrer for stirring, and bagging the stirred feed for packaging. In the laying duck feed, the conversion rate, the stability of feed quality and the albumen using amount of coarse dregs are improved by using additives to reduce the cost of the feed, and the growth expression of laying ducks also can be maintained or improved.

The invention relates to the fields of gene engineering and fermentation engineering and specifically relates to an acidic beta-glucanase NGlu as well as a gene and application thereof. The invention provides a novel beta-glucanase NGlu with an amino acid sequence as shown in SEQ ID NO.1; furthermore, the invention also provides a gene GNGlu for encoding the novel beta-glucanase, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 2; the invention also provides a recombinant vector and a recombinant bacterial strain both comprising the gene and application of the recombinant vector and the recombinant bacterial strain. The novel beta-glucanase is capable of achieving a relatively high fermentation level by virtue of efficient expression, so that the production cost can be reduced to a large extent in industrial production; as a result, the novel beta-glucanase has excellent application prospect in industries such as feed and beer brewing.