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Compound microbial bacterial preparation and application thereof

A technology of complex microbial agent and viable count, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, to achieve good results, shorten anaerobic digestion time, and accelerate enrichment

Inactive Publication Date: 2012-05-09
江苏加德绿色能源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no related reports that a compound microbial agent that is generally adapted to excess sludge and / or kitchen waste and can greatly promote biogas production is directly added to excess sludge and / or kitchen waste to promote biogas fermentation

Method used

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  • Compound microbial bacterial preparation and application thereof
  • Compound microbial bacterial preparation and application thereof
  • Compound microbial bacterial preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 fermented liquid

[0036] This embodiment only takes Bacillus licheniformis (ATCC21416), Pseudomonas putida (ATCC15070), Enterobacter cloacae (ATCC12666) and Candida crassa (ATCC10642) as examples to describe the specific content of the invention in detail, and does not limit The selection of four sources of microorganisms in the composite microbial bacterial agent of the present invention.

[0037] (1) Activate the above Bacillus licheniformis (ATCC21416), Pseudomonas putida (ATCC15070), Enterobacter cloacae (ATCC12666) and Candida crassa (ATCC10642) respectively into their respective slant medium for preservation, 28-37 Cultivate for 16-24 hours.

[0038] Preservation of four kinds of microorganisms uses the slant medium formula as follows:

[0039] Bacillus licheniformis culture medium: 10 grams of peptone, 5 grams of glucose, 5 grams of yeast extract, 10 grams of sodium chloride, 1 gram of manganese sulfate, 15 grams of agar, 1000 m...

Embodiment 2

[0048] Example 2 Using a composite microbial agent to treat excess sludge by anaerobic fermentation to produce biogas

[0049] 1. The four kinds of microbial fermented liquids prepared in Example 1 are mixed uniformly by the following parts by weight to become a composite microbial bacterial agent: 8 parts by weight of Bacillus licheniformis fermented liquid, 5 parts by weight of Pseudomonas putida fermented liquid, 10 parts by weight of Enterobacter cloacae fermented liquid Parts by weight, 7 parts by weight of Candida crassa fermented liquid.

[0050] 2. Select 530kg of residual sludge from a sewage treatment plant (wet weight, total solid content 19.1%), divide it into 5 parts on average, add 400kg of water to each part to adjust its total solid content to 4%, and mix well.

[0051] 3. Add the liquid mixture obtained in step 2 into 5 identical 600L anaerobic digestion tanks (each digestion tank adds 420L liquid mixture), respectively add the methanogenic inoculum (selected ...

Embodiment 3

[0055] Example 3 Using a composite microbial agent to treat kitchen waste by anaerobic fermentation to produce biogas

[0056] 1. The four kinds of microbial fermented liquids prepared in Example 1 are mixed uniformly by the following parts by weight to become a composite microbial bacterial agent: 11 parts by weight of Bacillus licheniformis fermented liquid, 6 parts by weight of Pseudomonas putida fermented liquid, 8 parts by weight of Enterobacter cloacae fermented liquid parts by weight, 14 parts by weight of Candida crassa fermented liquid.

[0057] 2. Choose 130kg of kitchen waste from the canteen (wet weight, total solid content 24.5%), the main components are rice, meat, vegetables, etc., remove hard objects such as bones and fully pulverize them with a crusher, and divide them into 2 servings.

[0058] 3. Add 200 kg of water to each portion of crushed kitchen waste to adjust the total solid content to 6%, and stir well.

[0059] 4. Add the liquid mixture obtained in...

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Abstract

The invention belongs to the technical field of environment protection and discloses a compound microbial bacterial preparation and application thereof. The compound microbial bacterial preparation consists of fermentation liquor of four bacteria, i.e. Bacillus licheniformis, Pseudomonas putida, Enterobacter cloacae and Candida valida. The compound microbial bacterial preparation provided by the invention has strong digestion power for various types of cellulose, proteins and fat macromolecules. The addition of the compound microbial bacterial preparation into excess sludge and / or kitchen waste can quicken enrichment of methanogen, shorten anaerobic digestion time and improve methane yield. Moreover, the synergetic effect of the four microbial strains is superior to the effect of any one, two or three strains of microorganisms.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a composite microbial bacterial agent and its application, in particular to a biodegradable sludge and / or kitchen waste that promotes anaerobic fermentation of excess sludge and / or kitchen waste to produce gas Composite microbial agent with biogas capacity and its application. Background technique [0002] In recent years, with the sustained and rapid development of my country's economy, energy shortage and environmental pollution have become two bottlenecks for my country's sustainable development. 95% of the energy currently used is fossil energy, such as oil, coal, natural gas, etc. These fossil fuels are non-renewable energy sources. Use also caused serious environmental pollution simultaneously. At the Copenhagen Conference, the Chinese government promised that by 2020, carbon dioxide emissions per unit of GDP will drop by 40% to 45% compared to 2005. This ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/16C12P5/02C02F11/04C12R1/10C12R1/40C12R1/01C12R1/72
CPCY02E50/343Y02E50/30Y02W10/20
Inventor 周楚新支银芳李登奎
Owner 江苏加德绿色能源有限公司
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