39 results about "Bacillus safensis" patented technology

The invention discloses a broth for preventing and treating ralstonia solanacearum and a preparation method thereof. According to the invention, different biocontrol fungus are combined, then mixed according to different proportions and fermented, and a stable and effective combination is screened, the screened combination can effectively control the ralstonia solanacearum through a root watering application method; the broth for preventing and treating ralstonia solanacearum is prepared by fermenting streptomyces pluricolorescens seed liquid, streptomyces recifensis seed liquid, a bacillus safensis broth, a brevibacilluscentrosporus broth, trichoderma rufobrunneum seed liquid, cane molasses, soybean molasses, cottonseed meal powder, composite amino acid raw powder, potassium fulvic acid, calcium carbonate, magnesium sulfate, and 800 parts of water.

The invention discloses Bbacillus safensis, a microbial agent, application of the Bbacillus safensis and the microbial agent, and a table vinegar preparation method. The Bacillus safensis is preservedin the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.18727, and the preservation date is October 23rd, 2019. The invention also discloses the microbialagent containing the Bacillus safensis. The invention discloses the application of the Bacillus safensis and the microbial agent in the field of food and table vinegar brewing. The invention further discloses the table vinegar brewing method. The Bacillus safensis disclosed by the invention has high-yield amylase activity and high-yield protease activity in vinegar brewing. Compareed with a control group, the starch utilization rate is increased by 18.04%, the protease activity can reach 860.62 U / mL fermentation liquor, starch and protein utilization rates of vinegar brewing can be obviously increased, precipitation is reduced, the content of amino acid in table vinegar is increased, the product flavor is improved, and the product quality is improved.

The invention discloses a bacillus safensis strain ZDC-02, and belongs to the technical field of biological control of plant diseases, wherein the strain is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.12217. Experiments indicate that the bacillus safensis strain ZDC-02 has the characteristics of being rapid in growth speed, broad in action spectrum, strong in stress resistance, capable of achieving rapid mass colonization on plant bodies and the like; therefore, the strain has a good application prospect. A bio-control preparation, as a bio-control preparation which has a quite broad application prospect and is prepared from the bacillus safensis, not only can effectively prevent and control various plant diseases but also can effectively improve a crop yield.

The invention discloses bacillus safensis and an application thereof. The strain is classified and named as bacillus safensis JX9 and is collected in China General Microbiological Culture Collection Center (CGMCC) in No.3, No.1 Courtyard, Beichen West Road, Chaoyang District, Beijing, with collection date of August 28, 2014 and collection number of CGMCC No.9617. The strain has good phosphate solubilizing effects. Ultraviolet spectrophotometry shows that the strain has the capacity of producing auxin. Research results show that the strain JX9 has potential application value in promoting tobacco growth.

The invention discloses a microbial fertilizer for control of potato scab and a preparation method thereof. The raw materials for preparation of the microbial fertilizer include: Bacillus aryabhattai, Bacillus safensis powder, Streptomyces nashvillensis powder, Streptomyces bellus, potassium fulvate, and cottonseed powder. The strains for compounding the microbial fertilizer have the characteristics of strong adaptability, good stability, coexistence, separability from soil, and strong bacteriostasis to pathogenic bacteria, also make rational use of the interaction between microorganisms, and combine with high-tech production and fermentation technology to produce the high quality product. The microbial fertilizer can effectively antagonize the pathogenic bacteria of potato scab, and has the effects of fixing nitrogen, stimulating plant growth, and improving crop quality, etc., thereby achieving the purposes of production increase and high yield.

The invention discloses a method for screening and purifying bunge pricklyash leaf endophytic bacillus safensis GHF4 and an application thereof, the bacillus safensis is preserved in China Center for Type Culture Collection with a preservation number CCTCC NO: M2014535, and is named as GHF4. The method is characterized by comprising the following steps: taking fresh bunge pricklyash leaf, disinfecting and cleaning, performing plate enrichment culture, performing gradient dilution plate coating, primary screening, secondary screening and the like to realize screening and purifying. its fermentation metabolites has strong antioxidant activity, when the concentration of a broth is 3.75mg / mL, clearance to ABTS free radical by endophytic bacillus safensis can reach 99.48%, and IC50 value is 0.80mg / mL. The endophytic bacillus safensis has good application prospect on production aspect of a natural anti-oxidant.

The invention provides bacillus safensis X3 and application thereof, belonging to the technical field of new use of microorganisms. The bacillus safensis X3 has an ability to degrade phosphorus, and the concentration of the degraded phosphorus reaches 476.6mg / L or above; the difficult-to-use phosphorus is converted into available phosphorus, so that the content of the available phosphorus in the soil is effectively increased, the utilization rate of fertilizer is improved, and the growth and development and fertilizer absorption of plants are promoted; furthermore, the concentration of indoleacetic acid produced by the bacillus safensis X3 is 34.78mu g / mL or above. The bacillus safensis X3 is applied to plant cultivation, and can significantly improve the yield and quality of grain crops such as wheat and corn, oil crops such as peanut, and cash crop such as tobacco.

The invention provides a microbial agent. The microbial agent comprises a culture medium and thalli, wherein the thalli comprise Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus safensis. The microbial agent provided by the invention can obtain a good plant seedling index and further improve yield and quality of vegetables, thereby having broad application prospects in the field of vegetable seedling raising. In addition, by using the microbial agent provided by the invention, a plant rhizosphere microbial environment can be improved, and beneficial bacteria are increased, thereby inhibiting the growth of harmful bacteria and further improving the disease resistance of plants.

The invention discloses bacillus safensis HMD9204 as well as a fungicide and application thereof. A strain is preserved in the Guangdong microbial culture collection center on December 18th, 2017, with the collection number of GDMCC No.60302. The strain cooperates with organic fertilizer or farmyard manure to achieve a very obvious prevention and control effect on eggplant fusarium wilt and bananavascular wilt, reduces the incidence rate of the eggplant fusarium wilt to 2%-5%, and reduces the incidence rate of the banana vascular wilt to 6.7%-9.6%.

The invention discloses bacillus safensis for producing protease and resisting botrytis cinerea. The bacterial strain is classified and named as Bacillus safensis SH180 and is preserved in CGMCC (China General Microbiological Culture Collection Center) on April 8, 2018; the preservation number is CGMCC15565. The bacterial strain has the effects of promoting the biological growth, producing the protease and resisting the botrytis cinerea. The bacterial strain is applied to production; important significance is realized for improving the tomato yield, reducing the harm of pesticide to irrigationwater bodies and reducing the generation of drug-resistance bacteria.

The invention provides bacillus safensis the preservation number of which is CGMCC NO.8843. The invention further provides a microbial agent which contains thallus and a culture medium; the thallus comprises bacillus safensis the preservation number of which is CGMCC NO.8843. The invention further provides application of the bacillus safensis and the microbial agent in preparation of a microbial fertilizer. By means of the technical scheme, the fertilizer efficiency of the phosphatic fertilizer can be increased more effectively.

The invention provides a microbial agent. The microbial agent comprises a culture medium and thalli, wherein the thalli comprise Bacillus subtilis, Baclicus lincheniformis and Bacillus safensis. The microbial agent provided by the invention can obtain a good plant seedling index and further improve yield and quality of vegetables, thereby having broad application prospects in the field of vegetable seedling raising. In addition, by using the microbial agent provided by the invention, a plant rhizosphere microbial environment can be improved, and beneficial bacteria are increased, thereby inhibiting the growth of harmful bacteria and further improving the disease resistance of plants.

The invention discloses preparation and application of a bacterial strain HCX-01 of verticillium dahliae capable of preventing and treating cotton verticillium wilt. The bacterial strain disclosed bythe invention is bacillus safensis HCX-01, belongs to clostridium selected from a resistant cotton root soil strain in a cotton test field of Nanyang Normal University and has a preservation number ofCCTCC NO. M2018153. The bacterial strain HCX-01 provided by the invention is applied to not only the inhibition of verticillium dahliae, but also the prevention and the treatment of the cotton verticillium wilt. The preparation and the application of the bacterial strain HCX-01, disclosed by the invention, have the beneficial effects that the HCX-01 can be cultured on an artificial culture medium, is easy to obtain and culture and facilitates factory production, popularization and application; after being treated by fermentation liquor of the HCX-01 for 12 hours, a verticillium dahliae sporesuspension has a spore germination rate of only 9.7 percent, which has a better inhibiting effect than that with the blank 39.6 percent spore germination rate; the harm of the verticillium dahliae tocotton is effectively reduced; meanwhile, compared with a chemical agent, the HCX-01 solves the problems of environmental protection and the like.

The invention relates to Bacillus safensis having a growth promoting function, bactericide and application thereof. Bacillus safensis AMCC101030 is collected at the China General Microorganism Collection Center on Dec. 18th, 2017 under the collection number of CGMCC No.15071. The Bacillus safensis AMCC101030 strain is acquired by screening for the first time and has obvious growth promoting effect. When the strain is doped into a seedling culture matrix, the functional strain secretes growth promoting substances, so that growth of tomato seedling is promoted.

The invention provides bacillus safensis YJC-4. The class name is bacillus safensis and the bacillus safensis YJC-4 is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation date is April 15, 2016, the preservation address is #3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.12356. The strain has the following advantages that (1) the bio-control effect is good; in a pot culture experiment, YJC-4 bacterium liquid is used for treating tobacco plants inoculated with tobacco black shank bacteria and the prevention and control effect reaches 81.59 percent and is remarkably better than that of 58 percent metalaxyl.mancozeb and a biological preparation, namely bacillus subtilis; the bacillus safensis YJC-4 has a potential application value in the aspect of biological prevention and control of the tobacco black shank and has good development and application prospects; the biological prevention and control is environment-friendly, does not easily have drug resistance and is safe to people and livestock, and conditions are created for green and prevention and control development of tobaccos; (2) the growth promoting effect is good; the biological amount and agronomic characters of YJC-4 bacterium liquid tobacco plants are remarkably promoted and the promoting effect is remarkably different from a control group.

The invention relates to a synergic disease-resistant bacterium as well as a culture method and applications of the synergic disease-resistant bacterium. The synergic disease-resistant bacterium is Bacillus safensis, and is assigned with the microbial accession number of CGMCC No.7847. The culture method comprises the following steps: selecting an SD23 strain as a starting strain for screening ofa first round, preparing a bacterium suspension, sequentially carrying out natural separation screening, mutation treatment screening and culture and screening of high-concentration shenqinmycin culture medium, wherein the strain obtained by culture and screening of the high-concentration shenqinmycin culture medium is adopted as a starting strain for screening of the next round, and repeatedly carrying out the process for multiple rounds, thus obtaining the target strain. Compared with the prior art, the synergic disease-resistant bacterium has the advantages that the synergic disease-resistant bacterium has good drug resistance to shenqinmycin, and forms a mixed preparation with shenqinmycin, the use is safe, the prevention and treatment effects for rice sheath blight disease and watermelon fusarium wilt can be improved, the yield is increased, meanwhile, the use amount of shenqinmycin is reduced, so that the cost is reduced.

The invention provides a microbial agent for promoting yield increase and disease resistance of potatoes, which comprises bacillus subtilis, bacillus megatherium, bacillus mucilaginosus, paenibacilluspolymyxa, saccharomycetes, bacillus safensis and lactobacillus plantarum. The invention also provides a preparation method of the microbial agent. The microbial agent is used for potato planting. Potato has developed roots, is high in growth vigor and has leaves which are large and dark green, the decay of potato seedlings is 7-10 days later than that of a control group, the surface gloss of fruits is good, bacterial wilt does not occur, and compared with an inactivated microbial agent or a microbial agent not applied, the microbial agent has the advantages that the weight of a single fruit is averagely increased by 0.8 g, and 0.7-1.2 potatoes per plant are averagely increased. The microbial agent is used for potato planting, the average acre yield reaches 4800-4850 kg, the yield is increased by 24-25% or above, and the incidence rate of bacterial wilt is 0%.

The invention discloses a field planting culture method and application of intestinal probiotics of sanhuang broiler chickens. The method comprises the following steps of separating probiotic strainsfrom duodenum, jejunum, ileum and cecum of different intestinal segments of chicken intestines and chicken manure, comparing strain tolerance, enzyme production capacity and acid production capacity,culturing and screening out strains with high viable count and rich enzyme production capacity, and compounding and screening the strains; carrying out field planting culture and optimizing a compounding ratio to obtain a compound microbial agent which is prepared from bacillus haynesii, pediococcus acidilactici, bacillus safensis, propionibacterium freudennreichii and clostridium butyricum, wherein the compound microbial agent is applied to breeding of sanhuang broiler chicks, probiotics rapidly grow and breed in intestinal tracts of chicks, number of viable bacteria is large, digestion of chicks to feed is facilitated, and immunity of chicks is improved. Therefore, the survival rate of the chicks is remarkably increased, weight gain is promoted, and the microbial agent has wide application value in chick breeding.

The invention relates to a resveratrol zymophyte and application thereof. Classification of a bacterial strain is named as Bacillus-safensis, a storage unit is a general microorganism center of China Committee for Culture Collection of Microorganisms, preservation number is CGMCC No.13129, preservation address is No.3, No.1 Compound, Beichen West Road, Chaoyang District, Beijing, preservation date is October 21, 2016, and the application of the resveratrol zymophyte in fermented resveratrol is provided. A microbial transformation method is used for directly converting polydatin in polygonum cuspidatum crude medicinal materials to resveratrol. The method is simple, transformation process is easy to control, specificity of transformation reaction is stronger, separation and purification of products are convenient, yield and quality of resveratrol product are effectively improved, and added value of products is improved.

The invention relates to a bacterial strain for co-producing chitosanase and gamma-polyglutamic acid and application thereof, belongs to the field of biotechnology. The bacteria lstrain is bacillus safensis LZ303, and is separated from seabed sediments in a Yantai sea area and is classified and named as bacillus safensis. The bacillus safensis was deposited at the China General Microbiological Culture Collection Center on December 21, 2017, with a preservation No.: CGMCC No. 15111. The bacterial strain has dual synthesis ability of chitosanase and gamma-polyglutamic acid, and can produce chitosanase or gamma-polyglutamic acid separately by controlling culture mediums and culture conditions, and can also co-produce chitosanase and gamma-polyglutamic acid. In addition, the bacterial strain has good genetic stability, simple composition of the culture mediums and low raw material cost, high chitosanase production activity, can be secreted to the outside of cells, is simple and convenientfor separation and extraction, has strong substrate specificity and can be directly used for transformation and production of chitosan oligosaccharide, has high gamma-polyglutamic acid production capabilities, and has advantages in industrial applications.

The invention relates to the field of control of water environments by microorganisms, in particular to bacillus safensis and an application thereof. The bacillus safensis has been collected into Common Microorganism Center of China Committee for Culture Collection of Microorganisms on September 28th, 2016, with collection number of CGMCC No.13059. The method has the advantages that the indigenously-degradable strain is obtained through screening, is loaded, flocculated and encapsulated, and is applied to sea area to restore, so that the restoring strain can be furthest ensured to reach the specified submarine deposit during restoration, and the restoration effect of the strain is guaranteed; when the strain is fed, the complicated instrument equipment is not needed, and the feeding effect and restoration effect are guaranteed.

The invention discloses an abalone biological fermentation feed capable of inhibiting vibrio, and relates to the field of biological feeds. The abalone biological fermentation feed capable of inhibiting vibrio is composed of the following components by weight: 25-35 parts of starch, 25-35 parts of rape seed cake, 12-18 parts of marine fish bone meal, 16-22 parts of sea meal, 8-12 parts of stone powder, 2-4 parts of calcium dihydrogen phosphate, 0.15-0.25 part of compound enzyme, 0.12-0.25 part of vitamin E, 0.13-0.15 part of bacillus safensis and 0.06-0.12 part of lactobacillus acidophilus. According to the abalone biological fermentation feed, the starch, the rape seed cakes, the marine fish bone meal, the sea meal, the mountain flour, the monocalcium phosphate, the compound enzyme, the vitamin E, the bacillus safensis and the lactobacillus acidophilus are used as raw materials for preparing the abalone biological fermentation feed capable of inhibiting the vibrio; the abalone biological fermentation feed is rich in protein, amino acid and other nutrient substances easy to digest and absorb, improves the disease resistance of abalones, is high in digestion ability of abalones, can avoid impurities generated during feeding, ensures the water quality, inhibits abalone infection, and is beneficial to ecological and healthy culture.

The invention provides a method for converting and extracting resveratrol by means of bacteria. A fermentation medium with the addition of a substrate, namely a polygonum cuspidatum crude medicinal material, is inoculated with a bacillus safensis seed liquid, fermentation is conducted at the temperature of 28-37 DEGC for 6-15 hours, when the thalli concentration OD600nm is equal to 0.8-1.6, a product starts to be separated out, thalli, the substrate and the product are interacted, the product with the thalli as crystal nuclei is crystallized and separated out under the action of microorganisms, fermentation liquid is centrifuged, washed with distilled water and precipitated, an organic solvent is added into precipitates, one time of extraction and drying are conducted, and the resveratrol product can be obtained. The purity is larger than or equal to 99%. The method is simple in operation, mild in condition, short in conversion time and high in product yield.

The invention provides bacillus safensis YZS-C03 and application thereof, and belongs to the technical field of microorganisms. The bacillus safensis YZS-C03 is classified and named as bacillus safensis YZS-C03, and is preserved in the China General Microbiological Culture Collection Center on February 1, 2021, the preservation number is CGMCC No.21798, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus safensis YZS-C03 has a very good effect in the aspect of polyethylene plastic biodegradation, provides a new resource and a solution for biodegradation of polyethylene wastes in the environment, and has great application prospects.

The invention belongs to the technical field of potato scab prevention and treatment. The invention discloses a strain of bacillus safeensis T1-5, and the preservation number of the bacillus safeensis T1-5 is CGMCC (China General Microbiological Culture Collection Center) NO.23506. The invention further discloses a method for preparing the bacillus safeensis T1-5. The strain is preserved in the China General Microbiological Culture Collection Center on September 29, 2021, the preservation number is CGMCC No.23506, the preservation date is September 29, 2021, the preservation unit is called CGMCC for short, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus safensis T1-5 provided by the invention is simple to culture, short in period, free of ecological toxicity, resistant to saline and alkaline, rich in available carbon source variety, capable of effectively inhibiting pathogenic bacteria of the potato scab, and very wide in application prospect in biological prevention and control of the potato scab.

The invention belongs to the technical field of microorganism application, and particularly relates to bacillus safensis LG01 and application thereof. The invention discloses bacillus safensis LG01, and the preservation number of the bacillus safensis LG01 is CCTCC (China Center for Type Culture Collection) No: M 20211624. The bacillus safensis has multiple functional characteristics: (1) the bacillus safensis has inhibitory activity on fungi such as candida albicans and aspergillus niger; (2) the compound has inhibitory activity on gram-positive bacteria such as staphylococcus aureus, micrococcus luteus and listeria monocytogenes, gram-negative bacteria such as legionella pneumophila, legionella Israeli and legionella martensii, and gram-negative plant pathogenic bacteria such as ralstonia solanacearum; (3) protease can be produced; (4) cellulase can be produced; and (5) a strong-adhesion biofilm can be formed. The functional characteristics enable the bacillus safensis to have diversified application forms, such as preparation of plant colonizers, plant biocontrol agents, bio-organic fertilizers, microbial feeds and the like, and the bacillus safensis has wide application prospects.

The invention discloses a method for increasing potassium content of tobacco leaves by combining microbial bacterial fertilizer with microbial fermentation liquid. The method comprises the following steps: (1) preparing the microbial fermentation liquid; (2) preparing the bacterial fertilizer; (3) applying base fertilizer; (4) watering with the microbial fermentation liquid. The method utilizes bacillus safensis and bacillus tequilensis to prepare the microbial fermentation liquid and the bacterial fertilizer, and adopts a way of combining the microbial fermentation liquid and the bacterial fertilizer; the bacillus safensis and the bacillus tequilensis are applied respectively in solid state form and a liquid state form, and are promoted to colonize in the soil so as to finally form a dominant bacterial community; the bacterial fertilizer and the microbial fermentation liquid are organically combined together, so that the potassium content of the tobacco leaves can be effectively increased. The method reduces the consumption of chemical fertilizer and chemical pesticide, protects and promotes the growth of tobaccos, and promotes environmental friendliness.

The invention relates to Bacillus safensis XWY06 which is preserved in China Center for Type Culture Collection with the preservation number of CCTCC No: M 2019818. The strain has stronger functions of decomposing inorganic phosphorus and organic phosphorus and can generate indoleacetic acid, cellulase, protease and siderophore. The invention also relates to a microbial inoculum containing the strain and application of the strain in promoting plant growth and improving the stress resistance of plants, such as disease resistance and drought resistance.