Bacillus safensis X3 and application thereof
A technology for bacillus and strains, applied to bacillus saffordi strain X3 and its application field, and can solve the problems of limiting the application scope of indole acetic acid, cumbersome application and the like
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Embodiment 1
[0050] 1. Configuration of culture medium
[0051] Prepare the following media:
[0052] (1) PKO liquid medium (inorganic phosphorus bacteria medium)
[0053] Sodium chloride 0.3g, glucose 10g, potassium chloride 0.3g, tricalcium phosphate 5g, ammonium sulfate 0.5g, magnesium sulfate heptahydrate 0.3g, manganese sulfate 0.03g, ferrous sulfate heptahydrate 0.03g, distilled water 1000mL, adjust pH When the value reaches 7.0-7.2, sterilize at 115°C for 30min. Add 15g of agar powder to the culture medium to get PKO solid medium.
[0054] (2) LB liquid medium
[0055] Tryptone 10g, yeast powder 5g, sodium chloride 10g, water 1000mL, adjust the pH value to 7.0, and sterilize at 121°C for 20min. Add 15g of agar powder to the culture medium to get LB solid medium.
[0056] Next, the plant growth-promoting bacteria that can secrete indole acetic acid are screened out through qualitative determination and quantitative determination.
[0057] Table 1 Basic properties of the tested ...
Embodiment 2
[0066] Aerobic test
[0067] Pour the sterilized LB medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, use an inoculation needle to pick up the strain X3 cultured on the slant, and puncture and inoculate it into the above medium (must pierce to the bottom of the tube). Cultivate at 30°C, and observe the results in 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria.
[0068] The test results showed that the colony of bacterial strain X3 grew along the surface of the agar column, and there were also colonies growing in the puncture line, which was facultative anaerobic (see Table 2).
Embodiment 3
[0070] Determination of catalase
[0071] Put 1 drop of 3% HO on a clean slide 2 o 2 , take 1 ring of the slant culture of the strain X3LB cultivated for 18-24 hours, in H 2 o 2 Smear in the middle, if bubbles are produced, it is positive, otherwise it is negative.
[0072] The test results showed that strain X3 was positive for catalase (see Table 2).
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