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A kind of pseudobacillus pallidum strain x21 and its application

A pseudobacillus, X21 technology, applied to Pseudomonas pallidum strain X21 and its application field, can solve the problems of limiting the application range of indole acetic acid and cumbersome application

Active Publication Date: 2020-07-28
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the artificial synthesis of indole acetic acid has expanded its source, the spraying method and content need to be strictly controlled, and the application is cumbersome, which limits the application range of indole acetic acid
Although rhizobacteria that symbiotically interact with plants can solve this problem, there are no reports on rhizobacteria that have both phosphorus-solubilizing function and phytohormone production.

Method used

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  • A kind of pseudobacillus pallidum strain x21 and its application
  • A kind of pseudobacillus pallidum strain x21 and its application
  • A kind of pseudobacillus pallidum strain x21 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Configuration of culture medium

[0051] Prepare the following media:

[0052] (1) PKO liquid medium (inorganic phosphorus bacteria medium)

[0053] Sodium chloride 0.3g, glucose 10g, potassium chloride 0.3g, tricalcium phosphate 5g, ammonium sulfate 0.5g, magnesium sulfate heptahydrate 0.3g, manganese sulfate 0.03g, ferrous sulfate heptahydrate 0.03g, distilled water 1000mL, adjust pH When the value reaches 7.0-7.2, sterilize at 115°C for 30min. Add 15g of agar powder to the culture medium to get PKO solid medium.

[0054] (2) LB liquid medium

[0055] Tryptone 10g, yeast powder 5g, sodium chloride 10g, water 1000mL, adjust pH to 7.0, sterilize at 121°C for 20min. Add 15g of agar powder to the culture medium to get LB solid medium.

[0056] Next, the plant growth-promoting bacteria that can secrete indole acetic acid are screened out through qualitative determination and quantitative determination.

[0057] Table 1 Basic properties of the tested soil

[0058]...

Embodiment 2

[0066] Aerobic test

[0067] Pour the sterilized LB culture medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, use an inoculation needle to pick up the strain X21 cultured on the slant, and puncture and inoculate it into the above culture medium (must pierce to the bottom of the tube). Cultivate at 30°C, and observe the results in 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria.

[0068] The test results showed that bacterial strain X21 colonies grew along the surface of the agar column, and colonies also grew in the puncture line, which was facultative anaerobic (see Table 2).

Embodiment 3

[0070] Determination of catalase

[0071] Put 1 drop of 3% HO on a clean slide 2 o 2 , take 1 ring of slant culture of strain X21 LB cultivated for 18~24h, and put it in H 2 o 2 Smear in the middle, if bubbles are produced, it is positive, otherwise it is negative.

[0072] The test results showed that the strain X21 was positive for catalase (see Table 2).

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Abstract

The invention provides a Pseudomonas pallidum strain X21 and its application, belonging to the technical field of new applications of microorganisms. The strain X21 has the ability to decompose phosphorus, and the concentration of degraded phosphorus is more than 421.15 mg / L, which converts difficult-to-use phosphorus into available phosphorus, increases the content of available phosphorus in the soil, improves the utilization rate of fertilizers, and promotes the growth and development of plants and the absorption of fertilizers; at the same time, the concentration of indoleacetic acid produced by the strain X21 is above 45.65 μg / mL. The application of the Pseudomonas pallidum strain X21 in plant cultivation can improve the yield and quality of grain crops wheat and corn, oil crop peanuts and economic crop tobacco.

Description

technical field [0001] The invention belongs to the technical field of new applications of microorganisms, and in particular relates to a Pseudomonas pallidum strain X21 and its application. Background technique [0002] Sandy fluvo-aquic soil has strong permeability, good shaking culture, and aerobic microbial activities are dominant, which can promote the decomposition of organic matter and accelerate the mineralization of organic matter. And the soil is loose and easy to cultivate. The capillary action of the soil is strong, the water runs fast, and there is a phenomenon of "night tide". The suitable tillage period is also long, and the seedlings are easy to stand; but the nutrient content is low, the fertilizer retention performance is poor, and the crops are prone to defertilization and premature aging in the later stage. At present, most of the soil has been used as arable land, and its utilization rate reaches 92.3%. Targeted improvement measures in the future: pla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G7/06C12R1/01
CPCA01G7/06C12N1/20C12N1/205C12R2001/01
Inventor 韩燕来田志强姜瑛王祎李培培谭金芳李慧
Owner HENAN AGRICULTURAL UNIVERSITY
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