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Bacillus pallidus strain X21 and application thereof

A technology of pseudobacillus and X21 is applied to pseudobacillus pallor strain X21 and its application field, and can solve the problems of cumbersome application, limited application scope of indole acetic acid and the like

Active Publication Date: 2019-03-19
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the artificial synthesis of indole acetic acid has expanded its source, the spraying method and content need to be strictly controlled, and the application is cumbersome, which limits the application range of indole acetic acid
Although rhizobacteria that symbiotically interact with plants can solve this problem, there are no reports on rhizobacteria that have both phosphorus-solubilizing function and phytohormone production.

Method used

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  • Bacillus pallidus strain X21 and application thereof
  • Bacillus pallidus strain X21 and application thereof
  • Bacillus pallidus strain X21 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. The configuration of the culture medium

[0051] Prepare the following media:

[0052] (1) PKO liquid medium (inorganic phosphorus bacteria medium)

[0053] Sodium chloride 0.3g, glucose 10g, potassium chloride 0.3g, tricalcium phosphate 5g, ammonium sulfate 0.5g, magnesium sulfate heptahydrate 0.3g, manganese sulfate 0.03g, ferrous sulfate heptahydrate 0.03g, distilled water 1000mL, adjust pH Value to 7.0~7.2, sterilize at 115℃ for 30min. Add 15g agar powder to the culture broth to form the PKO solid medium.

[0054] (2) LB liquid medium

[0055] Tryptone 10g, yeast powder 5g, sodium chloride 10g, water 1000mL, adjust pH to 7.0, sterilize at 121℃ for 20min. Add 15g agar powder to the culture broth to form the LB solid medium.

[0056] Next, qualitative and quantitative determinations are used to screen out plant growth-promoting bacteria that can secrete indole acetic acid.

[0057] Table 1 Basic properties of test soil

[0058]

[0059] 2. Qualitative determination

[0060] T...

Embodiment 2

[0066] Aerobic test

[0067] Pour the sterilized LB medium into 3 sterilized test tubes, about 2 / 3 of the place, on the aseptic operation table, use the inoculation needle to pick the slant cultured strain X21, puncture and inoculate the above medium Medium (must be pierced to the bottom of the tube). Incubate at 30°C and observe the results in 3 to 7 days. Those growing on the surface of the agar column are aerobic bacteria, such as those growing along the puncture line are anaerobes or facultative anaerobes.

[0068] The test results showed that the bacterial colonies of strain X21 grew along the surface of the agar column, and colonies also grew in the puncture line, which was facultative anaerobic (see Table 2).

Embodiment 3

[0070] Determination of catalase

[0071] Put 1 drop of 3% H on a clean glass slide 2 O 2 , Take 1 loop of the slant culture of strain X21 LB cultivated for 18-24h, 2 O 2 Medium smear, if there are bubbles, it is positive, otherwise it is negative.

[0072] The test results showed that strain X21 was positive for contact enzymes (see Table 2).

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Abstract

The invention provides a bacillus pallidus strain X21 and an application thereof, and belongs to the technical field of new applications of microorganisms. The bacillus pallidus strain X21 has phosphate-dissolving ability, and concentration of degrading phosphate reaches more than 421.15 mg / L. Difficultly used phosphate is converted to available phosphate, content of the available phosphate in soil is increased, a utilization ratio of a fertilizer is improved, and growth and development of plants and absorption to the fertilizer are promoted. At the same time, concentration of heteroauxin generated by the strain X21 reaches 45.65 ug / mL or more. The bacillus pallidus strain X21 is applied for planting the plants, so yield and quality of food crops of wheat and corn, an oil crop of peanut and an industrial crop of tobacco are improved better.

Description

Technical field [0001] The invention belongs to the technical field of new uses of microorganisms, and specifically relates to a Pseudospora pallor strain X21 and applications thereof. Background technique [0002] Sandy fluvo-aquic soil has strong permeability, good shaking culture, and aerobic microbial activity is dominant, which can promote the decomposition of organic matter and accelerate the mineralization of organic matter. And the soil is loose and easy to cultivate. The soil capillary function is strong, the water moves fast, and there is a phenomenon of "night tide". The suitable tillage period is also long and seedlings are easy to stand; but the nutrient content is low, the fertilizer retention performance is poor, and the crops are easy to lose fertilizer and senility in the later stage. At present, most of the soil has been used as arable land, with a utilization rate of 92.3%, two crops a year, and an annual output of about 600kg per mu. Targeted improvement me...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G7/06C12R1/01
CPCA01G7/06C12N1/20C12N1/205C12R2001/01
Inventor 韩燕来田志强姜瑛王祎李培培谭金芳李慧
Owner HENAN AGRICULTURAL UNIVERSITY
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