Bacillus safensis and application thereof
A technology of Bacillus suffolus and its strains, applied in the application field of Bacillus suffolus and its growth-promoting characteristics, can solve problems such as soil damage, easy pollution of the environment, loss of soil organic matter, etc.
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[0024] In the following, the present invention will be further described in conjunction with the embodiments.
[0025] The Bacillus safensis JX9 of the present invention, preservation unit: General Microbiology Center (CGMCC) of China Microbial Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, preservation date: August 2014 On 28th, deposit number: CGMCCNo.9617.
[0026] (1) Screening of CGMCCNo.9617
[0027] In the process of enrichment of beef extract peptone liquid medium: dilution plate culture method separation, weigh 10g of fresh soil from Hunan Sangzhiguandi Ping tobacco field, and place it in a 250mL Erlenmeyer flask containing glass beads and 90mL sterile water, 170r / min oscillate for 30min, then press 10 -5 , 10 -6 , 10 -7 Dilute each step in a gradient, and apply 0.1 mL of each on a straw culture medium (300 g of fresh straw straw, 25 g of agar, 1000 mL of water, autoclaved at 121°C for 25 min.) Plate and inorg...
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