Field planting culture method and application of intestinal probiotics of sanhuang broiler chickens
A technology of intestinal probiotics and cultivation method, which is applied in the field of colonization and cultivation of intestinal probiotics of three-yellow broiler chicks, to achieve the effect of no antagonism, ensuring the growth of bacteria and helping digestion
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Embodiment 1
[0022] Example 1: Colonization and cultivation method of probiotic flora in the intestinal tract of chickens with three yellow meat
[0023] A method for colonizing and cultivating intestinal probiotic flora of three-yellow meat chicks, specifically comprising the steps of:
[0024] (1) Through the screening and separation of different intestinal segments of the duodenum, jejunum, ileum and cecum in the midgut of free-range chickens raised by farmers and herdsmen, and chicken manure, directional isolation of Bacillus, Lactobacillus, Bifidobacterium and Clostridium butyricum, Representative strains were purified and purified by morphological and staining comparisons for species identification.
[0025] (2) The above-mentioned spores, lactic acid bacteria, bifidobacteria and Clostridium butyricum that were screened were cultured as probiotics in the corresponding medium: the spores were cultured in LB liquid medium at 37°C and 180 rpm / min for 48 hours, and the lactic acid bacter...
Embodiment 2
[0029] Example 2: Colonization and cultivation method of intestinal probiotic flora of three-yellow meat chicks
[0030] (1) Isolation, purification and identification of strains
[0031] Buy free-range native chickens raised by farmers and herdsmen in the northern and southern Xinjiang, fast to it, and drink water for 3 days, so that the food in its intestinal tract is completely emptied. After the live chickens are slaughtered, select the duodenum, jejunum, ileum, cecum and chicken feces from different intestines of each chicken, separate them, use sterile cotton rope to tie up each intestine, and mark each intestine. Then use sterile scissors to cut out different intestinal segments, place them on sterile gauze, and then use sterile scissors to cut the intestines in a sterile environment, use a sterile spoon to pick up the adhesions on the surface of the intestinal wall, and place them on the belt. Add 50ml of sterile saline to a 50ml / 150ml Erlenmeyer flask with glass bead...
Embodiment 3
[0074] Example 3: Application of the compound microbial agent prepared by colonization and cultivation of probiotic flora in the intestinal tract of three-yellow broiler chicks
[0075] This example investigates the application effect of the compound microbial bacterial agent prepared by the method of colonization and cultivation of probiotic flora in the intestinal tract of three-yellow broiler chicks in the feeding of three-yellow broiler chickens. Six pens (average 1 Wanyu / Building), to compare the effects of bacterial agents on the growth performance and feed-to-meat ratio of broiler yellow chickens under different dilution concentration gradients, and set up three dilution gradients, 1:500 (T1), 1:1000 (T2), 1:1500 (T3), 1:2000 (T4) and 1:3000 (T5) ratio, fed with commercially available complex microbial agents according to the ratio of instructions as control group 1 (CK1) with normal feeding without adding bacterial agents as control group (CK2 ), monitor the growth per...
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