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Field planting culture method and application of intestinal probiotics of sanhuang broiler chickens

A technology of intestinal probiotics and cultivation method, which is applied in the field of colonization and cultivation of intestinal probiotics of three-yellow broiler chicks, to achieve the effect of no antagonism, ensuring the growth of bacteria and helping digestion

Pending Publication Date: 2021-01-15
乌鲁木齐菌之源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual application process, how to effectively control the number of viable bacteria and the stability of metabolites of each strain when different strains are compounded is a difficult problem in the development and application of microbial agents at present.

Method used

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  • Field planting culture method and application of intestinal probiotics of sanhuang broiler chickens
  • Field planting culture method and application of intestinal probiotics of sanhuang broiler chickens
  • Field planting culture method and application of intestinal probiotics of sanhuang broiler chickens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Colonization and cultivation method of probiotic flora in the intestinal tract of chickens with three yellow meat

[0023] A method for colonizing and cultivating intestinal probiotic flora of three-yellow meat chicks, specifically comprising the steps of:

[0024] (1) Through the screening and separation of different intestinal segments of the duodenum, jejunum, ileum and cecum in the midgut of free-range chickens raised by farmers and herdsmen, and chicken manure, directional isolation of Bacillus, Lactobacillus, Bifidobacterium and Clostridium butyricum, Representative strains were purified and purified by morphological and staining comparisons for species identification.

[0025] (2) The above-mentioned spores, lactic acid bacteria, bifidobacteria and Clostridium butyricum that were screened were cultured as probiotics in the corresponding medium: the spores were cultured in LB liquid medium at 37°C and 180 rpm / min for 48 hours, and the lactic acid bacter...

Embodiment 2

[0029] Example 2: Colonization and cultivation method of intestinal probiotic flora of three-yellow meat chicks

[0030] (1) Isolation, purification and identification of strains

[0031] Buy free-range native chickens raised by farmers and herdsmen in the northern and southern Xinjiang, fast to it, and drink water for 3 days, so that the food in its intestinal tract is completely emptied. After the live chickens are slaughtered, select the duodenum, jejunum, ileum, cecum and chicken feces from different intestines of each chicken, separate them, use sterile cotton rope to tie up each intestine, and mark each intestine. Then use sterile scissors to cut out different intestinal segments, place them on sterile gauze, and then use sterile scissors to cut the intestines in a sterile environment, use a sterile spoon to pick up the adhesions on the surface of the intestinal wall, and place them on the belt. Add 50ml of sterile saline to a 50ml / 150ml Erlenmeyer flask with glass bead...

Embodiment 3

[0074] Example 3: Application of the compound microbial agent prepared by colonization and cultivation of probiotic flora in the intestinal tract of three-yellow broiler chicks

[0075] This example investigates the application effect of the compound microbial bacterial agent prepared by the method of colonization and cultivation of probiotic flora in the intestinal tract of three-yellow broiler chicks in the feeding of three-yellow broiler chickens. Six pens (average 1 Wanyu / Building), to compare the effects of bacterial agents on the growth performance and feed-to-meat ratio of broiler yellow chickens under different dilution concentration gradients, and set up three dilution gradients, 1:500 (T1), 1:1000 (T2), 1:1500 (T3), 1:2000 (T4) and 1:3000 (T5) ratio, fed with commercially available complex microbial agents according to the ratio of instructions as control group 1 (CK1) with normal feeding without adding bacterial agents as control group (CK2 ), monitor the growth per...

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Abstract

The invention discloses a field planting culture method and application of intestinal probiotics of sanhuang broiler chickens. The method comprises the following steps of separating probiotic strainsfrom duodenum, jejunum, ileum and cecum of different intestinal segments of chicken intestines and chicken manure, comparing strain tolerance, enzyme production capacity and acid production capacity,culturing and screening out strains with high viable count and rich enzyme production capacity, and compounding and screening the strains; carrying out field planting culture and optimizing a compounding ratio to obtain a compound microbial agent which is prepared from bacillus haynesii, pediococcus acidilactici, bacillus safensis, propionibacterium freudennreichii and clostridium butyricum, wherein the compound microbial agent is applied to breeding of sanhuang broiler chicks, probiotics rapidly grow and breed in intestinal tracts of chicks, number of viable bacteria is large, digestion of chicks to feed is facilitated, and immunity of chicks is improved. Therefore, the survival rate of the chicks is remarkably increased, weight gain is promoted, and the microbial agent has wide application value in chick breeding.

Description

technical field [0001] The invention belongs to the field of cultivation methods of microbial flora, and in particular relates to a colonization cultivation method and application of probiotic flora in the intestinal tract of three-yellow broiler chicks. Background technique [0002] Microecological agent (Microbial ecological agent) is under the guidance of microecological balance theory, microecological control theory and microecological nutrition theory, etc., and selects beneficial microorganisms in animals and their special nutrients through special processing techniques such as cultivation, fermentation, and drying. The biological preparations or live bacterial agents produced can supplement the lack or reduction of normal microorganisms in the intestinal tract in terms of quantity or type, and maintain or adjust the balance of the micro-ecological environment in the intestinal tract. It has the functions of preventing and treating diseases and health care, and is non-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75C12R1/07C12R1/145C12R1/01
CPCC12N1/20A23K10/18A23K50/75
Inventor 曾军刘欢欢刘珍珠陈开旭高兴旺王为兰刘建伟
Owner 乌鲁木齐菌之源生物科技有限公司
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